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Coupling of TLC and UV-measurement for quantification of naproxen and its main metabolite in urine

  • E. M. Abdel-Moety
  • A. M. Al-Obaid
  • A. I. Jado
  • E. A. Lotfi
Original Papers

Summary

A simple sensitive method of high specificity and selectivity for quantitative determination of the non-steroidal anti-inflammatory drug naproxen and its main metabolite, 6-demethylated derivative, in biological specimens is described. Like naproxen, its metabolite absorbs maximally at 232 om; this makes their simultaneous quantification, via direct UV-measurements at λmax, in biological fluids quite impossible. Simple TLC-separation on silica gel F254 using chloroform + methanol (85:15, v/v) achieved the best fractionation of the unchanged drug and its metabolite from the matrix-contents of urine. UV-quantification of fractionated components could reach concentration levels of 0.2–3.0μg ml−1 (ppm) in worked up urine samples. Varying levels of unchanged antiinflammatory drug and the phenolic metabolite could be accurately traced in urine samples following a 2.9 mglk.g oral dose after different time-intervals. Synthetic preparation of the metabolite by demethylation of naproxen is briefly mentioned.

Key words

Naproxen metabolite nornaproxen TLC/UV-determination urine 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • E. M. Abdel-Moety
    • 1
  • A. M. Al-Obaid
    • 1
  • A. I. Jado
    • 1
  • E. A. Lotfi
    • 1
  1. 1.Dept. of Pharmaceutical Chemistry, College of PharmacyKing Saud UniversityRiyadhSaudi Arabia

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