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Pharmacokinetics of arginine and aspartic acid administered simultaneously in the rat: I plasma kinetics

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Summary

Arginine and aspartic acid pharmacokinetics have been studied in rats following the simultaneous oral and intravenous administration of an aqueous solution containing 0.1 mmol of radiolabeled amino acids (U-[14C]-arginine and [3H-aspartic acid) per rat.

The assays of total free arginine and aspartic acid have been carried out using a spectrophotometric technique. Exogenous fractions of the two amino acids have been determined by the isotopic dilution method.

Pharmacokinetic parameters of the two amino acids were estimated assuming a two compartment mammillary open system.

A significant increase of blood levels of these two amino acids has been observed. This increase was less marked via the oral route (bioavailability of 0.49 for arginine and 0.24 for aspartic acid). One minute after intravenous administration, about 12 % of each amino acid were found in the free form in the plasma, and the half-life of arginine was almost twice that of aspartic acid. After oral administration the opposite was observed.

The plasma level of these two amino acids has been observed to increase after administration by either route 400 minutes after administration.

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Correspondence to G. Campistron.

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Campistron, G., Guiraud, R., Cros, J. et al. Pharmacokinetics of arginine and aspartic acid administered simultaneously in the rat: I plasma kinetics. European Journal of Drug Metabolism and Pharmacokinetics 7, 307–313 (1982). https://doi.org/10.1007/BF03189634

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Key words

  • Arginine
  • aspartic acid
  • half-life
  • area under the curve
  • bioavailability