Effect of 1.1.1 trifluoro-N-(2-methyl-4-phenyl sulfonyl) methane sulfonamide (Destun 50WP) on rat hepatic microsomal enzymes, aniline hydroxylase and amino-pyrine N-demethylase

  • M. Ira Thabrew
  • Godwin O. Emerole
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Summary

Investigations have been carried out to determine the effects of the herbicide 1.1.1 trifluoro-N-(2-methyl-4-phenyl sulfonyl) methane sulfonamide (Destun) on some hepatic microsomal drug metabolizing enzymes in rat. Administration of 100 mg herbicide/kg rat (i.p. or oral) resulted in a stimulation of aniline hydroxylase and p-aminopyrene N-demethylase activities by 1.3 fold and 1.6 fold respectively. A dose-related increase in enzyme activities was observed with a maximum effect at about 100 mg Destun/kg rat. The increased microsomal protein content, liver weight: body weight ratio and decreased sleeping time in the herbicide-treated animals indicated the possibility of Destun being an “inducer”. Results of investigations on the kinetic properties of aniline hydroxylase and p-aminopyrene-N-demethylase on administration of Destun suggests that in addition to its inducer effect, the herbicide could stimulate the enzyme activities by decreasing the affinity of these enzymes for their respective substrates.

Key words

Perfluidone microsomal enzymes aniline hydroxylase aminopyrine N-demethylase 
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References

  1. 1.
    WHO (1975): Early detection of health impairment in occupational exposure to health hazards — Report of aWHO Study Group Tech. Rep. Seires No. 571.Google Scholar
  2. 2.
    WHO (1976): Occupational health problems and their control.WHO Chronicle 30: 318–324.Google Scholar
  3. 3.
    Lincer, J.L. and Peakall, D.B. (1970): Metabolic effects of polychlorinated biphenyls in the American kestrel.Nature. (London),228; 783–784.PubMedCrossRefGoogle Scholar
  4. 4.
    Fujita, S., Isuji, H., Kato, K., Saeki, S. and Tsukamoto, H. (1971): Effects of biphenyl chlorides on rat liver microsomes.Fukuoka Acta Med., 62; 30–34.Google Scholar
  5. 5.
    Villeneuve, D.C., Grant, D.L., Phillips, W.E.S., Clark, M.L. and Clegg, D.J. (1971): EfTects of PCB administration on microsomal enzyme activity in pregnant rabbits.Bull Environ. Contam. Toxicol. 6, 120–128.PubMedCrossRefGoogle Scholar
  6. 6.
    Litterst, C.L., Farber, T.M., Baker, A.M., and Van Loon, E.J. (1972): Effect of polychlorinated biphenyl on hepatic microsomal enzymes in the rat.Toxicol. App; Pharmacol. 23; 112–122.CrossRefGoogle Scholar
  7. 7.
    Johnstone, G.J., Ecobichon, D.J. and Hutzinger, O. (1974): The influence of pure polychlorinated biphenyl compounds on hepatic function in the rat.Toxicol. Appt. Pharmacol. 28, 68–81.Google Scholar
  8. 8.
    Bruckner, J.V., Khanna, K.L. and Cornish, H.H. (1974): Polychlorinated biphenyl-induced alteration of biologic parameters in the rat.Toxicol. Appl. Pharmacol. 28; 180–199.CrossRefGoogle Scholar
  9. 9.
    3M Company (1976): International Technical Data Bulletin on Destun herbicide.Google Scholar
  10. 10.
    Sakpere, S. and Olorunsogo, O. (1981): Unpublished results.Google Scholar
  11. 11.
    Holtman, J.L., Gram, T.E., Gibon, P.L. and Gillette, J.R. (1968): The distribution of the components of the mixed function oxidase between the rough and smooth endoplasmic reticulum of liver cells.Biochem. J., 110, 407–412.Google Scholar
  12. 12.
    Schenkman, J.B., Remmer, H., and Estabrook, R.W. (1967): Spectral studies of drug interactions with hepatic microsomal cytochrome.Mol. Pharmacol., 3: 113–123.Google Scholar
  13. 13.
    La Du, B.N., Gaudette, L., Trousof, N. and Brodie, B.B. (1955): Enzymatic dealkylation of aminopyrine (pyramidon) and other alkyl amines.J. Biol. Chem., 214; 741.Google Scholar
  14. 14.
    Nash, T. (1953): The colorimetric estimation of formaldehyde by means of the Hantzech reaction.Biochem. J., 55. 416–421.PubMedGoogle Scholar
  15. 15.
    Gornal, A.G. and Badawill, C.J. and David, M.M. (1949): Determination of serum proteins by means of Biuret reaction.J. Biol. Chem. 177; 751.Google Scholar
  16. 16.
    Ernster, L. and Orrenius, S. (1965): Substrate-induced synthesis of the hydroxylating enzyme system of liver microsomes.Fed. Proc., 24; 1190–1199.PubMedGoogle Scholar
  17. 17.
    Gillette, J.R., Conney, A.H., Cosmides, G.J., Estabrook, R.W., Fouts, J.R. and Mannering, G.J. (1975):Microsomes and Drug oxidations. 1st Ed. Academic Press, New York.Google Scholar
  18. 18.
    Bickers, D.R., Harber, L.C., Kappas, A. and Alvares, A.P. (1972): Polychlorinated biphenyls: Comparative effects of high and low chlorine containing Aroclors on hepatic mixed function oxidase.Res. Comm. Chem. Pathol. Pharmacol., 3: 505–512.Google Scholar
  19. 19.
    Litterst, C.L. and Van Loon, E.J. (1974): Time course of induction of microsomal enzymes following treat-ment with polychlorinated biphenyl.Bull. Environ. Contam. Toxicol. 11; 206–212.PubMedCrossRefGoogle Scholar
  20. 20.
    Williams, R.T. (1959):Detoxication Mechanisms. 2nd Ed. Chapman and Hall, London.Google Scholar
  21. 21.
    Gelboin, H.V. (1972): Studies on the mechanisms of microsomal hydroxylase induction and its role in carcinogenic action.Rev. Can. Biol. 31; 39.PubMedGoogle Scholar
  22. 22.
    Gelboin, H.V., Kinoshita, N. and Wiebel, F.J. (1972): Microsomal hydroxylase: induction and role in polycyclic hydrocarbon carcinonogenesis and toxicity.Fed. Proc. 31; 1298.PubMedGoogle Scholar
  23. 23.
    Alvares, A.P., Schillings, G.R. and Kuntznam, R. (1968): Differences in the kinetics of benzpyrene hydroxylation by hepatic drug metabolizing enzymes from phénobarbital and 3-methylcho!anthrene treated rat.Biochem. Biophys. Res. Communie. 30; 588.CrossRefGoogle Scholar
  24. 24.
    Gnosspelias, Y., Thoer, H. and Orrenius, S. (1979/70): A comparative study on the effects of phénobarbital and 3,4-benzpyrene on the hydroxylating enzyme systems of rat liver microsomes.Chem. Biol. Interact. 1; 125.CrossRefGoogle Scholar
  25. 25.
    Mannering, G.J. (1971): Properties of cytochrome P-450 as affected by environmental factors: qualitative changes due to administration of polycyclic hydrocarbons.Metabolism.20; 228.PubMedCrossRefGoogle Scholar
  26. 26.
    Conney, A.H., Lu, A.Y.H., Levin, A., Somogyi, A., West, S., Jacobson, M., Ryan, D. and Kuntzman, R. (1973): Effect of enzyme inducers on substrate specificity of the cytochrome p-450’s.Drug Metab. Disp. 1; 199.Google Scholar
  27. 27.
    Wayne, I.G. (1925): Metabolic fate of perfluidone in a lactating Cowat.J. Agric-Fed. Chem. 23; 869–872.Google Scholar
  28. 28.
    Paulson, G.D., Jacobson, A.M., and Zaylskie, R.G. (1977): Herbicide Perfluidone: 1.1.1 trifluoro-N-(2-methyl-4-phenyl sulfonyl) methane sulfonamide: its metabolism in rats and chickens.Pest. Biochem. Physiol. 7; 62–72.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • M. Ira Thabrew
    • 1
  • Godwin O. Emerole
    • 1
  1. 1.College of Medecine, Department of BiochemistryUniversity of IbadanNigeria

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