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HMW glutenin subunits in multiploidAegilops species: composition analysis and molecular cloning of coding sequences

Abstract

TheAegilops genus contains species closely related to wheat. In common with wheat,Aegilops species accumulate high molecular weight (HMW) glutenin subunits in their endospermic tissue. In this study, we investigated the composition of HMW glutenin subunits in four multiploidAegilops species using SDS-PAGE analysis. Furthermore, by working withAe. ventricosa, we established an efficient genomic PCR condition for simultaneous amplification of DNA sequences coding for either x-or y-type HMW glutenin subunits from polyploidAegilops species. Using the genomic PCR condition, we amplified and subsequently cloned two DNA fragments that may code for HMW glutenin subunits inAe. ventricosa. Based on an analysis of the deduced amino acid sequences, we concluded that the two cloned sequences encode one x- and one y-type of HMW glutenin subunit, respectively.

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Correspondence to Daowen Wang.

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Xie, R., Wan, Y., Zhang, Y. et al. HMW glutenin subunits in multiploidAegilops species: composition analysis and molecular cloning of coding sequences. Chin.Sci.Bull. 46, 309–313 (2001). https://doi.org/10.1007/BF03187192

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Keywords

  • Aegilops
  • Ae. ventricosa
  • HMW glutenin subunit
  • molecular cloning