Hydrodynamics-based administration via tail vein was used to deliver naked plasmid with human factor IX (hFIX) cDNA in 2.2 mL Ringer’s solution into mice within 7 s. The peak level of expression of hFIX was 2921 ng/mL in mouse plasma. The hFIX cDNA expression increased with increasing the amount of plasmid DNA injected. The peak level of gene expression declined after repeated injection of plasmid (1459 ng/mL). The hFIX cDNA was detected in various organs, but the highest level of gene expression appeared in liver. Transaminase levels and liver histological results showed that rapid intravenous plasmid injection into mice induced transient focal acute liver damage, which was rapidly repaired within 3–10 d. These results suggested that high-level expression of hFIX cDNA can be achieved by hydrodynamics-based plasmid transfer and this method is now further used for gene therapy and gene function study in our lab.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Wolff, J. A., Malone, R. W., Williams, P. et al., Direct gene transfer into mouse musclein vivo, Science, 1990, 247: 1465–1468.
Budker, V., Zhang, G., Knechtle, S. et al., Naked DNA delivered intraportally expresses efficiently in hepatocytes, Gene Ther, 1996, 3: 593–598.
Zhang, G. F., Vargo, D., Budker, V. et al., Expression of naked plasmid DNA injected into the afferent and efferent vessels of rodent and dog livers, Hum. Gene Ther, 1997, 8: 1763–1772.
Liu, F., Song, Y., Liu, D., Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA, Gene Ther, 1999, 6: 1258–1266.
Zhang, G. F., Budker, V., Wolff, J. A., High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA, Hum. Gene Ther., 1999, 10: 1735–1737.
Zhang, G. F., Song, Y. K., Liu, D., Long-term expression of human alphal-antitrypsin gene in mouse liver achieved by intravenous administration of plasmid DNA using a hydrodynamics-based procedure, Gene Ther., 2000, 7: 1344–1349.
Wang, P., Dai, Y. F., Qiu, X. F. et al., High expression of human factor IX cDNA driven by HCMV promotor in mammalian cells, Chinese Science Bulletin, 1992, 37(1): 52–55.
Wang, H. W., Bao, Y. Lu, D. R. et al., High expression of human clotting factor IX cDNA in myoblasts C2C12 cells and CH3 mice, Science in China, Ser. C., 1997, 40(4): 371–378.
Budker, V., Budker, T., Zhang, G. et al., Hypothesis: naked plasmid DNA is taken up by cellsin vivo by a receptor-mediated process, J. Gene Med., 2000, 2: 76–78.
Wang, H.W., Wang, F., Chen, L. et al., The transfer and expression of human clotting factor IX in muscle mediated by electroporation, Chin. J. Med. Genet., 2001, 18: 476–478.
Wang, Q., Lu, D. R., Xing, Y. N. et al., Establishment of the recombinant adenovirus-mediated gene transfer system and expression of hFIXin vivo andin vitro, Fudan Journal, 1996, 35: 601–606.
Ge, Y., Powell, S., Van Roey, M. et al., Factors influencing the development of an anti-factor IX (FIX) immune response following administration of adeno-associated virus-FIX, Blood, 2001, 97: 3733–3737.
Chen, Z.Y, Yant, S. R., He, C. Y. et al., Linear DNAs concate merizein vivo and result in sustained transgene expression in mouse liver, Mol. Ther, 2001, 3: 403–410.
Miao, C. H., Thompson, A. R., Loeb, K. et al., Long-term and therapeutic-level hepatic gene expression of human factor IX after naked plasmid transferin vivo, Mol. Ther, 2001 3: 947–957.
Waterhouse, P. M., Wang, M. B., Lough, T., Gene silencing as an adaptive defence against viruses, Nature, 2001, 411: 834–842.
Qin, L., Ding, Y., Pahud, D. R. et al., Promoter attenuation in gene therapy: interferon-γ and tumor necrosis factor-α inhibit transgene expression, Hum. Gene Ther, 1997, 8: 2019–2029.
Harms, J. S., Splitter, G. A., Interferon-γ inhibits transgene expression driven by SV40 or CMV promoters but augments expression driven by the mammalian MHCI promoter, Hum. Gene Ther., 1995, 6: 1291–1297.
Clark, A. J., Harold, G., Yull, F. E., Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice, Nucleic Acids Res., 1997, 25: 1009–1014.
Song, Y. K., Liu, F., Zhang, G. et al., Hydrodynamics-based transfection: Simple and efficient method for introducing and expressing transgenes in animals by intravenous injection of DNA, Methods Enxymol, 2002, 346: 92–105.
Jiang, J., Yamato, E., Miyazaki, J., Intravenous delivery of naked plasmid DNA forin vivo cytokine expression, Biochem. Biophys. Res. Commun., 2001, 289: 1088–1092.
Holst, H. U., Dagnaes-Hansen, F., Corydon, T. J. et al., LDL receptor-GFP fusion proteins: new tools for the characterisation of disease-causing mutations in the LDL receptor gene, Eur. J. Hum., 2001, 9: 815–822.
Chang, J., Sigal, L. J., Lerro, A. et al., Replication of the human hepatitis delta virus genome is initiated in mouse hepatocytes following intravenous injection of naked DNA or RNA sequences, J. Virol., 2001, 75: 3469–3473.
Yang, J., Chen, S., Huang, L. et al., Sustained expression of naked plasmid DNA encoding hepatocyte growth factor in mice promotes liver and overall body growth, Hepatology, 2001, 33: 848–859.
Liu, F., Huang, L., Electric gene transfer to the liver following systemic administration of plasmid DNA, Gene Ther, 2002, 9: 1116–1119.
Vorup-Jensen, T., Jensen, U. B., Liu, H. et al., Tail-vein injection of mannan-binding lectin DNA leads to high expression levels of multimeric protein in liver, Mol. Ther, 2001, 3: 867–874.
Maruyama, H., Higuchi, N., Nishikawa, Y. et al., High-level expression of naked DNA delivered to rat liver via tail vein injection, J. Gene Med., 2002, 4: 333–341.
Stoll, S. M., Sclimenti, C. E., Baba, E. J. et al., Epstein-Barr virus/human vector provides high-level, long-term expression of alphal-antitrypsin in mice, Mol. Ther, 2001, 4: 122–129.
Yant, S. R., Meuse, L., Chiu, W. et al., Somatic integration and long-term transgene expression in normal and haemophilic mice using a DNA transposon system, Nat. Genet., 2000, 25: 35–41.
Cui, F. D., Kishida, T., Ohashi, S. et al., Highly efficient gene transfer into murine liver achieved by intravenous administration of naked Epstein-Barr virus (EBV)-based plasmid vectors, Gene Ther, 2001, 8: 1508–1513.
About this article
Cite this article
He, C., Feng, D., Wu, W. et al. Efficient expression of human factor IX cDNA in liver mediated by hydrodynamics-based plasmid administration. Chin.Sci.Bull. 48, 790–795 (2003). https://doi.org/10.1007/BF03187054
- gene transfer
- naked plasmid
- hydrodynamics-based transfection