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Isolation and identification of genes expressed differentially in rice inflorescence meristem with suppression subtractive hybridization

Abstract

A subtracted cDNA library of rice (Oryza sativa L.) inflorescence meristem (EM) was constructed using the suppression subtractive hybridization (SSII) method. The cDNAs of the rice shoot apical meristem (SAM) were used as “driver” and inflorescence meristem (IM) as “tester” in the experiment, respectively. Forty of 250 randomly chosen cDNA clones were identified by differential screening, which were IM-specific or IM-highly expressed. Most of the rice IM cDNAs cloned by SSH appear to represent rare transcripts, 40% of which were derived from truly differentially expressed genes. Of all the forty sequenced cDNA inserts, eleven contain the regions with 60%–90% identity to their homolog in GenBank, eighteen are expected to be new genes, only two correspond to published rice genes.

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Author information

Correspondence to Jinshui Yang.

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Liu, J., Liu, J., Yuan, Z. et al. Isolation and identification of genes expressed differentially in rice inflorescence meristem with suppression subtractive hybridization. Chin.Sci.Bull. 46, 98–100 (2001). https://doi.org/10.1007/BF03187000

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Keywords

  • rice (Oryza sativa L.) cDNA
  • suppression subtractive hybridization
  • shoot apical meristem
  • inflorescence meristem