Advertisement

Journal of Physiology and Biochemistry

, Volume 65, Issue 4, pp 377–386 | Cite as

Possible mechanisms of weight loss of Siberian hamsters (Phodopus sungorus sungorus) exposed to short photoperiod

  • C. AtgiéEmail author
  • P. Sauvant
  • L. Ambid
  • C. Carpéné
Article

Abstract

Several weeks of short day photoperiod (SD) exposure promote a dramatic decrease of white adipose tissue (WAT) mass in Siberian hamsters(Phodopus sungorus sungorus). This slimming effect is accompanied by changes in the adipocyte responsiveness to adrenergic stimulation that are still under debate. We investigated whether possible changes in the antilipolytic responses, and/or lipogenic activities could be involved in such lipid deposition/mobilisation imbalance. Male Siberian hamsters were exposed for 11 weeks to SD or long day photoperiod and basal or stimulated lipolytic and lipogenic activities were measured on white adipocytes. As expected, the body mass of SD-animals was decreased. Besides a slight reduction in the basal lipolysis and in the maximal response to dibutyryl-cAMP, the responses to adrenergic and non-adrenergic lipolytic agents (forskolin, adenosine deaminase) were similar in both groups. Fat mass loss was likely not resulting from changes in the lipolytic responses of adipocytes to biogenic amines (e.g. octopamine), which were unaltered, or to a direct lipolytic stimulation by melatonin or histamine, which were inactive. Antilipolytic responses to insulin or tyramine were slightly decreased in SD-adipocytes. Basal or insulin-stimulated lipid accumulation in WAT, measured by glucose incorporation into lipids, did not change after SD-exposure. However, a significant decrease in the lipoprotein lipase activity was observed in the WAT of SDanimals. Despite the observed changes, the weight loss of SD-exposed Siberian hamsters was likely not resulting only from impaired antilipolytic orde novo lipogenic activities in white adipocytes, but either from other dramatic changes occurring during seasonal photoperiod-sensitive body weight regulation.

Key words

Adipocyte Lipoprotein lipase Catecholamines PIA β3-adrenergic receptors Melatonin Photoperiod Hamsters 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Atgié, C., Le Gouic, S., Marti, L., Hanoun, N., Casteilla, L., Penicaud, L., Ambid, L. and Carpené, C. (1998): Lipolytic and antilipolytic responses of the Siberian hamster (Phodopus sungorus sungorus) white adipocytes after weight loss induced by short photoperiod exposure. Comp Biochem Physiol A Mol Integr Physiol, 119, 503–510.CrossRefPubMedGoogle Scholar
  2. 2.
    Bairras, C., Ferrand, C. and Atgié, C. (2003): Effect of tyramine, a dietary amine, on glycerol and lactate release by isolated adipocytes from old rats. J Physiol Biochem, 59, 161–167.CrossRefPubMedGoogle Scholar
  3. 3.
    Barrett, P., van den Top, M., Wilson, D., Mercer, J.G., Song, C. K., Bartness, T.J., Morgan, P.J. and Spanswick, D. (2009): Short photoperiod-induced decrease of histamine H3 receptors facilitates activation of hypothalamic neurons in the Siberian hamster. Endocrinology, 150, 3655–363.CrossRefPubMedGoogle Scholar
  4. 4.
    Bartness, T.J., Elliott, J.A. and Goldman, B.D. (1989): Control of torpor and body weight patterns by a seasonal timer in Siberian hamsters. Am J Physiol, 257, R142–149.PubMedGoogle Scholar
  5. 5.
    Bartness, T.J. and Wade, G.N. (1985): Body weight, food intake and energy regulation in exercising and melatonin-treated Siberian hamsters. Physiol Behav, 35, 805–808.CrossRefPubMedGoogle Scholar
  6. 6.
    Bowers, R.R., Gettys, T.W., Prpic, V., Harris, R.B. and Bartness, T.J. (2005): Short photoperiod exposure increases adipocyte sensitivity to noradrenergic stimulation in Siberian hamsters. Am J Physiol Regul Integr Comp Physiol, 288, R1354–1360.PubMedGoogle Scholar
  7. 7.
    Butler, M.P. and Zucker, I. (2009): Seasonal pelage changes are synchronized by simulated natural photoperiods in Siberian hamsters (Phodopus sungorus). J Exp Zool A Ecol Genet Physiol, 311, 475–482.CrossRefPubMedGoogle Scholar
  8. 8.
    Carpené, C., Bour, S., Visentin, V., Pellati, F., Benvenuti, S., Iglesias-Osma, M.C., GarcÍa-Barrado, M.J. and Valet, P. (2005): Amine oxidase substrates for impaired glucose tolerance correction. J Physiol Biochem, 61, 405–419.CrossRefPubMedGoogle Scholar
  9. 9.
    Carpené, C., Bousquet-Melou, A., Galitzky, J., Berlan, M. and Lafontan, M. (1998): Lipolytic effects of beta 1-, beta 2-, and beta 3-adrenergic agonists in white adipose tissue of mammals. Ann N Y Acad Sci, 839, 186–189.CrossRefPubMedGoogle Scholar
  10. 10.
    Dole, V.P. and Meinertz, H. (1960): Microdetermination of long-chain fatty acids in plasma and tissues. J Biol Chem, 235, 2595–2599.PubMedGoogle Scholar
  11. 11.
    Ferrand, C., Redonnet, A., Prevot, D., Carpené, C. and Atgié, C. (2006): Prolonged treatment with the beta3-adrenergic agonist CL 316243 induces adipose tissue remodeling in rat but not in guinea pig: 1) fat store depletion and desensitization of beta-adrenergic responses. J Physiol Biochem, 62, 89–99.CrossRefPubMedGoogle Scholar
  12. 12.
    Heldmaier, G., Steinlechner, S., Rafael, J. and Vsiansky, P. (1981): Photoperiodic control and effects of melatonin on nonshivering thermogenesis and brown adipose tissue. Science, 212, 917–919.CrossRefPubMedGoogle Scholar
  13. 13.
    Lafontan, M. (2008): Advances in adipose tissue metabolism. Int J Obes (Lond), 32, S39–51.CrossRefGoogle Scholar
  14. 14.
    Le Gouic, S., Delagrange, P., Atgié, C., Nibbelink, M., Hanoun, N., Casteilla, L., Renard, P., Lesieur, D., Guardiola-Lemaitre, B. and Ambid, L. (1996): Effects of both a melatonin agonist and antagonist on seasonal changes in body mass and energy intake in the garden dormouse. Int J Obes Relat Metab Disord, 20, 61–67.Google Scholar
  15. 15.
    Marti, L., Morin, N., Enrique-Tarancon, G., Prevot, D., Lafontan, M., Testar, X., Zorzano, A. and Carpené, C. (1998): Tyramine and vanadate synergistically stimulate glucose transport in rat adipocytes by amine oxidase-dependent generation of hydrogen peroxide. J Pharmacol Exp Ther, 285, 342–349.PubMedGoogle Scholar
  16. 16.
    Mercer, J.G., Duncan, J.S., Lawrence, C.B. and Trayhurn, P. (1994): Effect of photoperiod on mitochondrial GDP binding and adenylate cyclase activity in brown adipose tissue of Djungarian hamsters. Physiol Behav, 56, 737–740.CrossRefPubMedGoogle Scholar
  17. 17.
    Moody, A.J., Stan, M.A., Stan, M. and Gliemann, J. (1974): A simple free fat cell bioassay for insulin. Horm Metab Res, 6, 12–16.CrossRefPubMedGoogle Scholar
  18. 18.
    Morgan, P. J., Ross, A.W., Mercer, J.G. and Barrett, P. (2006): What can we learn from seasonal animals about the regulation of energy balance?Prog Brain Res, 153, 325–337.CrossRefPubMedGoogle Scholar
  19. 19.
    Paul, M.J., Zucker, I. and Schwartz, W. J. (2008): Tracking the seasons: the internal calendars of vertebrates. Philos Trans R Soc Lond B Biol Sci, 363, 341–361.CrossRefPubMedGoogle Scholar
  20. 20.
    Prevot, D., Soltesz, Z., Abello, V., Wanecq, E., Valet, P., Unzeta, M. and Carpené, C. (2007): Prolonged treatment with aminoguanidine strongly inhibits adipocyte semicarbazide-sensitive amine oxidase and slightly reduces fat deposition in obese Zucker rats. Pharmacol Res, 56, 70–79.CrossRefPubMedGoogle Scholar
  21. 21.
    Rodbell, M. (1964): Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis. J Biol Chem, 239, 375–380.PubMedGoogle Scholar
  22. 22.
    Savard, R., Deshaies, Y., Despres, J.P., Marcotte, M., Bukowiecki, L., Allard, C. and Bouchard, C. (1984): Lipogenesis and lipoprotein lipase in human adipose tissue: reproducibility of measurements and relationships with fat cell size. Can J Physiol Pharmacol, 62, 1448–1452.PubMedGoogle Scholar
  23. 23.
    Song, C.K. and Bartness, T.J. (2001): CNS sympathetic outflow neurons to white fat that express MEL receptors may mediate seasonal adiposity. Am J Physiol Regul Integr Comp Physiol, 281, R6–672.Google Scholar
  24. 24.
    Velkov, Z.A., Velkov, Y., Galunska, B.T., Paskalev, D.N. and Tadjer, A.V. (2009): Melatonin: Quantum-chemical and biochemical investigation of antioxidant activity. Eur J Med Chem, 44, 2834–2839.CrossRefPubMedGoogle Scholar
  25. 25.
    Wade, G.N. and Bartness, T.J. (1984): Effects of photoperiod and gonadectomy on food intake, body weight, and body composition in Siberian hamsters. Am J Physiol, 246, R26–30.PubMedGoogle Scholar

Copyright information

© Universidad de Navarra 2009

Authors and Affiliations

  1. 1.UMR 5248, Chemistry of Biology of Membranes and Nanoobjects (CBMN), CNRSUniversity Bordeaux 1, ENITABPessacFrance
  2. 2.INSERM U858, I2MRUniversity Paul SabatierToulouseFrance

Personalised recommendations