Reconstruction of human nuclear transfer embryos is a necessary step of therapeutic cloning. In this study we injected somatic cell nuclei into M II oocytes and activated reconstructed oocytes with calcium ionophore A23187 (CaA) and 6-dimethy laminopurine (6-DMAP). After oocyte activation and 2PN formation, we removed the female PN. By using this method, we avoided the application of DNA fluorescent stain and ultraviolet light for oocyte enucleation, and over elimination of ooplasm was also mitigated. Some reconstructed embryos developed into the blastocyst stagein vitro.
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Lu, C., Lin, G., Xie, C. et al. Reconstruction of human embryos derived from somatic cells. Chin.Sci.Bull. 48, 1840–1842 (2003). https://doi.org/10.1007/BF03184065
- nuclear transfer
- somatic cell