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Chinese Science Bulletin

, Volume 48, Issue 18, pp 1982–1987 | Cite as

Involvement of Jasmonate-signaling pathway in the herbivore-induced rice plant defense

  • Tao Xu
  • Qiang Zhou
  • Wei Chen
  • Guren Zhang
  • Guofeng He
  • Dexiang Gu
  • Wenqing ZhangEmail author
Reports

Abstract

The expression patterns of eight defenserelated genes in the herbivore-infested and jasmonatetreated (jasmonic acid, JA and its derivative MeJA) rice leaves were analyzed using RT-PCR. The results showed thatSpodoptera litura Fabricius (Lepidoptera: Noctuidae) herbivory induced the expression of lipoxygenase (LOX) and allene oxide synthase (AOS) genes that are involved in the jasmonate-signaling pathway. Moreover,S. litura damage resulted in the expression of farnesyl pyrophosphate synthase (FPS), Bowman-birk proteinase inhibitor (BBPI), phenylalanine ammonia-lyase (PAL) and other rice defenserelated genes that were also induced by aqueous JA treatment or gaseous MeJA treatment. These indicated that in rice leaves, the JA-related signaling pathway was involved in theS. litura-induced chemical defense. Mechanical damage and brown planthopper (BPH),Nilaparvata lugens (Stål) (Homoptera: Delphacidae) damage induced the expression ofLOX gene, but both treatments did not induce the expression ofAOS gene. However, BPH damage induced the expression of acidic pathogen-related protein 1 (PR1a), Chitinase (PR-3), andPAL genes, which is involved in the salicylatesignaling pathway. It was suggested that salicylate-related signaling pathway or other pathways, rather than jasmonate-signaling pathway was involved in the BPH-induced rice plant defense.

Keywords

rice brown planthopper Spodoptera litura jasmonate signaling pathway gene expression 

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Copyright information

© Science in China Press 2003

Authors and Affiliations

  • Tao Xu
    • 1
  • Qiang Zhou
    • 1
  • Wei Chen
    • 1
  • Guren Zhang
    • 1
  • Guofeng He
    • 1
  • Dexiang Gu
    • 1
  • Wenqing Zhang
    • 1
    Email author
  1. 1.State Key Laboratory for Biocontrol & Institute of EntomologySun Yat-set (Zhongshan) UniversityGuangzhouChina

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