Involvement of Jasmonate-signaling pathway in the herbivore-induced rice plant defense
- 105 Downloads
The expression patterns of eight defenserelated genes in the herbivore-infested and jasmonatetreated (jasmonic acid, JA and its derivative MeJA) rice leaves were analyzed using RT-PCR. The results showed thatSpodoptera litura Fabricius (Lepidoptera: Noctuidae) herbivory induced the expression of lipoxygenase (LOX) and allene oxide synthase (AOS) genes that are involved in the jasmonate-signaling pathway. Moreover,S. litura damage resulted in the expression of farnesyl pyrophosphate synthase (FPS), Bowman-birk proteinase inhibitor (BBPI), phenylalanine ammonia-lyase (PAL) and other rice defenserelated genes that were also induced by aqueous JA treatment or gaseous MeJA treatment. These indicated that in rice leaves, the JA-related signaling pathway was involved in theS. litura-induced chemical defense. Mechanical damage and brown planthopper (BPH),Nilaparvata lugens (Stål) (Homoptera: Delphacidae) damage induced the expression ofLOX gene, but both treatments did not induce the expression ofAOS gene. However, BPH damage induced the expression of acidic pathogen-related protein 1 (PR1a), Chitinase (PR-3), andPAL genes, which is involved in the salicylatesignaling pathway. It was suggested that salicylate-related signaling pathway or other pathways, rather than jasmonate-signaling pathway was involved in the BPH-induced rice plant defense.
Keywordsrice brown planthopper Spodoptera litura jasmonate signaling pathway gene expression
Unable to display preview. Download preview PDF.
- 6.Walling, L. L., The myriad plant responses to herbivores, J. Plant Growth Regul., 2000, 19: 195–216.Google Scholar
- 10.Ozawa, R., Arimura, G., Takabayashi, J. et al., Involvement of jasmonateand salicylate-related signaling pathways for the production of specific herbivore-induced volatiles in plants, Plant Cell Physiol., 2000, 41: 391–398.Google Scholar
- 14.Lou, Y. G., Cheng, J. A., Ping, X. F. et al., Discrimination by the egg parasitoid Anagrus nilaparvatae between two hosts,Nilaparvata lugens andSogatella furcifera, Acta Entomol. Sin., 2002, 45(6): 770–776.Google Scholar
- 26.Thaler, J. S., Jasmonic acid mediated interactions between plants, herbivore, parasitoids, and pathogen: a review of field experiments in tomato, in Inducible Plant Defenses Against Pathogens and Herbivores: Biochemistry, Ecology, and Agriculture (eds. Agrawal, A. A., Tuzun, S., Bent, E.), St. Paul, MN: American Phytopathological Society Press, 1999, 319–334.Google Scholar