Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

IGF-II and IGFBP-1 reversely regulate blastocyst implantation in mouse

  • 24 Accesses


Insulin-like growth factor (IGF)-II and IGF binding protein (IGFBP)-1, members of IGF family, are important in the cyclic development of endometrium and the blastocyst implantation. In the present study, the indirect immunofluorescence showed that IGF-II and IGFBP-1 were specifically expressed at the maternal-fetal interface. In a co-culture system, IGF-II significantly enhanced the attachment and outgrowth of the blastocyst on monolayer of uterine epithelial cells, while IGFBP-1 did not affect the blastocyst attachment, but markedly inhibited the blastocyst outgrowth. The results of zymography showed that IGF-II enhanced the activities of MMP-2 and MMP-9, while IGFBP-1 did not affect the activities of MMP-2 and MMP-9. In conclusion, the equilibrium between the invasion of trophoblast and the inhibition of deciduas may be regulated by the interaction between the IGF-II-expressing invading cytotrophoblast and maternal deciduas-derived IGFBP-1.

This is a preview of subscription content, log in to check access.


  1. 1.

    Han, V. K., Basset, K., Walton, J. et al., The expression of insulin-like growth factor (IGF) and IGF binding protein genes in the human placenta and membranes: evidence for IGF:IGFBP interactions at the feto-maternal interface, J. Clin. Endocinol. Metab., 1996, 81: 2680.

  2. 2.

    Giudice, L. C., Martina, N. A., De Las Fuentes, L. et al., Insulin-like growth factor binding protein-1(IGFBP-1) at the maternal-fetal interface and IGF-I, IGF-II and IGFBP-1 in the circulation of women with severe pre-eclampsia, Am. J. Obstet. Gynecol., 1997, 176: 751.

  3. 3.

    Fazeabas, A. T., Donnelly, K. M., Hild-Petito, S. et al., Secretory proteins of the baboon endometrium: regulation during the menstrual cycle and early pregnancy, Hum. Reprod. Update., 1997, 3: 533.

  4. 4.

    Irvin, K., Giudice, L. C., IGFBP-1 binds to the a 5 β 1 integrin in human cytotrophoblasts and inhibits their invasion into decidualized endometrium stromal cells in vitro, GH & IGF Res., 1998, 8: 21.

  5. 5.

    Zeng, G. Q., Cao, Y. J., Co-culture of mouse embryos with monolayer of uterine epithlial cells, Acta Zoologica Sinica, 1996, 42: 212.

  6. 6.

    Graham, C. H., McCrae, K. R., Lala, P. K., Mocular mechanism controlling trophoblast invasion of the uterus, Troph. Res., 1993, 7: 237.

  7. 7.

    Simon, C., Gimeno, M. J., Mercader, A. et al., Cytokines-adhesion molecules-invase proteinases. The missing paracrine/autocrine link in embryonic implantation, Mol. Hum. Reprod., 1996, 2: 405.

  8. 8.

    Cai, L. Q., Cao, Y. J., Duan, E. K., Role of ανβ3 integrin in embryo implantation in the mouse, Chinese Science Bulletin, 2000, 45(15): 1639.

  9. 9.

    Samani, A. A., Pnina, B., The receptor for the type I insulin-like growth factor and its ligands regulate multiple cellular functions that impact on metastasis, Cancer Metastasis: Biological and Clinical Aspects, 2001, 10: 289.

  10. 10.

    Lee, O. H., Bae, S. K., Bae, M. H. et al., Identification of angiogenic properties of insulin-like growth factor II in vitro angiogenesis models, Br. J. Cancer, 2000, 82: 385.

  11. 11.

    Tie, G. D., Cao, Y. J., Zhao, X. X. et al., Induction of matrix metalloproteinase-9 and -2 activity in mouse blastocyst by fibronectin-integrin interaction, Chinese Science Bulletin, 2000, 45: 475.

  12. 12.

    Zhang, J., Tie, G. D., Cao, Y. J. et al., The influence of leukamemia inhibitor factor and fibronectin on gene expression of metalloproteinase, Chinese Science Bulletin, 2001, 46: 401.

  13. 13.

    Bischof, P., Meisser, A., Campana, A., Involvement of trophoblast in embryo implantation: regulation by paracrine factors, J. Reprod. Immunol., 1998, 39: 167.

  14. 14.

    Manes, S., Mira, E., Barbacid, M. M., Identification of insulin-like growth factor-binding protein-1 as a potential physiological substrate for human stromelysin-3, J. Biol. Chem., 1997, 25706.

  15. 15.

    Maquoi, E., Polette, M., Nawrocki, B. et al., Expression of stromelysin-3 in the human placenta and placental bed, Placeta, 1997, 18: 277.

  16. 16.

    Gibson, J. M., Aplin, J. D., White, A., Regulation of IGF bioavailability in pregnancy, Molecular Human Reproduction, 2001, 7: 79.

Download references

Author information

Correspondence to Enkui Duan.

About this article

Cite this article

Tian, Y., Tie, G., Cao, Y. et al. IGF-II and IGFBP-1 reversely regulate blastocyst implantation in mouse. Chin.Sci.Bull. 47, 1816–1820 (2002). https://doi.org/10.1007/BF03183849

Download citation


  • IGF-II
  • IGFBP-1
  • implantation
  • MMP-2
  • MMP-9