Chinese Science Bulletin

, Volume 50, Issue 20, pp 2328–2336 | Cite as

Development, chromosome location and genetic mapping of EST-SSR markers in wheat

  • Haimei Chen
  • Linzhi Li
  • Xianyun Wei
  • Sishen Li
  • Tiandong Lei
  • Haizhou Hu
  • Honggang Wang
  • Xiansheng Zhang
Articles

Abstract

A number of 151695 wheat expression sequence tags (ESTs) that originated from GenBank/dbEST from July 14, 2003 to August 24, 2004 were used to search for simple sequence repeats (SSRs) with motif 2–5 bp, and 2038 simple sequence repeats (EST-SSRs), which accounted for 1.34% of EST database, were identified. Based on these SSR sequences, 249 EST-SSR primer pairs and 166 amplified clear bands in various wheat cultivars were designed. These EST-SSR markers can be used as new molecular markers in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 EST-SSR primer pairs and 193 EST-SSR loci were located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic framework map previously constructed using recombinant inbred lines.

Keywords

wheat EST-SSR primer nulli-tetrasome RIL genetic map 

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References

  1. 1.
    Tautz, D., Renz, M., Simple sequences are ubiquitous repetitive components of eukaryotic genomes, Nucleic Acids Res., 1984, 12: 4127–4138.CrossRefGoogle Scholar
  2. 2.
    Tautz, D., Hypervariability of simple sequences as a general source for polymorphic DNA markers, Nucleic Acids Res., 1989, 7: 6463–6471.CrossRefGoogle Scholar
  3. 3.
    Powell, W., Machray, G. C., Provan, J., Polymorphism revealed by simple sequence repeats, Trends Plant Sci., 1996, 7: 215–222.Google Scholar
  4. 4.
    Decroocq, V., Fave, M. G., Hagen, L. et al., Development and transferability of apricot and grape EST microsatellite markers across taxa, Theor. Appl. Genet., 2003, 106: 912–922.Google Scholar
  5. 5.
    Cordeiro, G. M., Casu, R., McIntyre, C. L. et al., Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum, Plant Sci., 2001, 160: 1115–1123.CrossRefGoogle Scholar
  6. 6.
    Eujayl, I., Sorrells, M. E., Baum, M. et al., Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat, Theor. Appl. Genet., 2002, 104: 399–407.CrossRefGoogle Scholar
  7. 7.
    Gupta, P. K., Rustgi, S., Sharma, S. et al., Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat, Mol. Genet. Genomics, 2003, 270: 315–323.CrossRefGoogle Scholar
  8. 8.
    Gao, L. F., Tang, J. F., Li, H. W. et al., Analysis of microsatellites in major crops assessed by computational and experimental approaches, Mol. Breed., 2003, 12: 245–261.CrossRefGoogle Scholar
  9. 9.
    Yu, J. K., Dake, T. M., Singh, S. et al., Development and mapping of EST-derived simple sequence repeat markers for hexaploid wheat, Genome, 2004, 47: 805–818.CrossRefGoogle Scholar
  10. 10.
    Hackauf, B., Wehling, P., Identification of microsatellite polymorphismsin an expressed portion of the rye genome, Plant Breed, 2002, 121: 17–25.CrossRefGoogle Scholar
  11. 11.
    Thiel, T., Michalek, W., Varshney, R. K. et al., Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.), Theor. Appl. Genet., 2003, 106: 411–422.Google Scholar
  12. 12.
    Barrett, B., Griffiths, A., Schreiber, M. et al., A microsatellite map of white clover, Theor. Appl. Genet., 2004, 109: 596–608.Google Scholar
  13. 13.
    Cho, Y. G., Ishii, T., Temnykh, S. et al., Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.), Theor. Appl. Genet., 2000, 100: 713–722.CrossRefGoogle Scholar
  14. 14.
    Han, Z. G., Guo, W. Z., Song, X. L. et al., Genetic mapping of EST-derived microsatellites from the diploidGossypium arboreum in allotetraploid cotton, Mol. Genet. Gen., 2004, 272: 308–327.Google Scholar
  15. 15.
    Rungis, D., Berube, Y., Zhang, J. et al., Robust simple sequence repeat markers for spruce (Picea spp.) from expressed sequence tags, Theor. Appl. Genet., 2004, 109: 1283–1294.CrossRefGoogle Scholar
  16. 16.
    Röder, M. S., Korzun, V., Wendehake, K. et al., A microsatellite map of wheat, Genetics, 1998, 149: 2007–2023.Google Scholar
  17. 17.
    Pestsova, E., Ganal, M. W., Roder, M. S., Isolation and mapping of microsatellite markeras specific for the D genome of bread wheat, Genome, 2000, 43: 689–697.CrossRefGoogle Scholar
  18. 18.
    Nagaoka, T., Ogihara, Y., Application of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers, Theor. Appl. Genet., 1997, 94: 597–602.CrossRefGoogle Scholar
  19. 19.
    Lu, R. H., Meng, J. L., MapDraw: A microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage map, Hereditas, 2003, 25: 317–321.Google Scholar
  20. 20.
    Gao, L. F., Jing, R. L., Huo, N. X. et al., One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat, Theor. Appl. Genet., 2004, 108: 1392–1400.CrossRefGoogle Scholar
  21. 21.
    Temnykh, S., Park, W. D., Ayres, N. et al., Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.), Theor. Appl. Genet., 2000, 100: 697–712.CrossRefGoogle Scholar
  22. 22.
    Chin, E. C. L., Maize simple repetitive DNA sequences: Abundance and allele variation, Genome, 1996, 39: 866–873.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2005

Authors and Affiliations

  • Haimei Chen
    • 1
  • Linzhi Li
    • 1
  • Xianyun Wei
    • 1
  • Sishen Li
    • 1
  • Tiandong Lei
    • 1
  • Haizhou Hu
    • 1
  • Honggang Wang
    • 1
  • Xiansheng Zhang
    • 1
  1. 1.College of AgronomyShandong Agricultural University/Shandong Key Lab of Crop BiologyTai’anChina

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