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Protein tyrosine phosphatases involved in signaling of the ABA-induced H2O2 generation in guard cells ofVicia faba L.

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Chinese Science Bulletin

Abstract

Although protein tyrosine phosphatases (PTPases) play an important role in signal transduction in animal cells, little is known about the function of PTPases in higher plants. Hydrogen peroxide (H2O2) and mitogen-activated protein kinases (MAPKs) are the critical components of ABA signaling pathway in guard cells. PTPase is an important regulator of MAPK, which is believed to mediate ABA-induced H2O2 generation in guard cells ofVicia faba L. Here, we investigate the possible role of PTPases in stomatal movement process. Phenylarsine oxide (PAO), a specific inhibitor of PTPases, could prevent ABA or H2O2-induced stomatal closure ofVicia faba L; furthermore, it could promote opening of the stomata closed by ABA or H2O2. The activity of PTPases can be effectively inhibited by PAO and H2O2. DTT had no effect on the PAO-induced inhibition of PTPases activity, but it could relieve the inhibition of H2O2 on PTPases activity. PAO could also inhibit the ABA-induced H2O2 generation in guard cells ofVicia faba L. These results suggested that PTPases is a critical signaling component in ABA-induced stomatal closure, and serve as targets for H2O2 lying on the signaling pathways downstream of ABA induced H2O2 generation.

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Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094, Beijing, China

    Wuliang Shi & Shuqiu Zhang

  2. College of Agronomy and Biotechnology, China Agricultural University, 100094, Beijing, China

    Wensuo Jia

  3. College of Life, Laiyang Agricultural College, 265200, Laiyang, China

    Xin Liu

Authors
  1. Wuliang Shi
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  2. Wensuo Jia
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  3. Xin Liu
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  4. Shuqiu Zhang
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Correspondence to Shuqiu Zhang.

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Shi, W., Jia, W., Liu, X. et al. Protein tyrosine phosphatases involved in signaling of the ABA-induced H2O2 generation in guard cells ofVicia faba L. . Chin.Sci.Bull. 49, 1841–1846 (2004). https://doi.org/10.1007/BF03183411

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  • DOI: https://doi.org/10.1007/BF03183411

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