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Cloning and enzymology analysis of farnesyl pyrophosphate synthase gene from a superior strain ofArtemisia annua L.


A cDNA(af1) encoding farnesyl pyrophosphate synthase AaFPS1 (FPS, EC2.5.1.1/EC2.5.1.10) from a high yieldArtemisia annua strain 025 has been cloned from its cDNA library. Sequence analysis showed that the cDNA encoded a protein of 343 amino acid (aa) residues with molecular weight of 39 kD. Deduced aa sequence of the cDNA was similar to FPS from other plants, yeast and mammals, containing 5 conserved domains found in both prenyl transferase and polyprenyl synthase. The expression of the cDNA inEscherichia coli showed measurable specific activity of FPSin vitro. The enzyme was purified by ion exchange chromatography and its kinetics was measured. These results would further promote the molecular regulation of artemisinin biosynthesis.

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  1. 1.

    Chappell, J., The biochemistry and molecular biology of isoprenoid metabolism, The Plant Physiol., 1995, 107: 1.

  2. 2.

    Chen, D. H., Ye, H. C., Li, G. F., Expression of a chimeric farnesyl diphosphate synthase gene inArtemisia annua L. transgenic plants viaAgrobacteriumtumefaciens-mediated transformation, Plant Science, 2000, 155: 179.

  3. 3.

    Chen, D. H., Ye, H. C., Li, G. F. et al., Advances in molecular biology of plant isoprenoid metabolic pathway, Acta Botanica Sinica (in Chinese), 2000, 42(6): 551.

  4. 4.

    Mercke, P., Crock, J., Corteau, R. et al., Cloning, expression, and characterization of epi-cedrol synthase, a sesquiterpene cyclase fromArtem isia annua L., Archives of Biochemistry and Biophysics, 1999, 369(2): 213.

  5. 5.

    Chen, D. H., Liu, C. J., Ye, H. C. et al., Ri-mediated transformation ofArtemisia annua with a recombinant farnesyl diphosphate synthase gene for artemisinin production, Plant Cell, Tissue and Organ Culture, 1999, 57: 157.

  6. 6.

    Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature(London), 1970, 227: 680.

  7. 7.

    Reed, B. C., Rilling, H. C., Crystallization and partial characterization of prenyltransferase from Avian liver, Biochemistry, 1975, 14: 50.

  8. 8.

    Attucci, S., Aitken, S. M., Ibrahim, R. K. et al., A cDNA encoding farnesyl pyrophosphate synthase in White Lupin, Plant Physiol., 1995, 108: 835.

  9. 9.

    Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, 72: 248.

  10. 10.

    Linsheng, S., Poulter, C. D., Yeast farnesyl-diphosphate synthase: Site-directed mutagenesis of residues in highly conserved prenyltransferase domains I and II, Proc. Natl. Acad. Sci. USA, 1994, 91: 3044.

  11. 11.

    Yasuhiko, M., WonKyung, K., Barry, V. C., Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua, Gene., 1996, 172: 207.

  12. 12.

    Chen, A., Kroon, P. A., Poulter, C. D., Isoprenyl diphosphate synthases: Protein sequence comparisons, a phylogenetic tree, and predictions of secondary structure, Protein Sci., 1994, 3: 600.

  13. 13.

    Tarshis, L. C., Mujing, Y., Poulter, C. D., Crystal structure of recombinant farnesyl diphosphate synthase at 2.6 Å resolution, Biochemistry, 1994, 33: 10871.

  14. 14.

    Nuria, C., Montserrat, A., Didier, D. et al.,Arabidopsis thaliana contains two differentially expressed farnesyl-diphosphate synthase genes, Journal of Biological Chemistry, 1996, 271(13): 7774.

  15. 15.

    Chen, X. Y., Ye, H. C., Plant secondary metablism and its regulation, in Advances in Plant Sciences (in Chinese), Beijing: Higher Education Press, 1998, 1: 293.

  16. 16.

    Chang, J. L., Peter, H., Xia, Y. C., Expression pattern of genes encoding farnesyl diphosphate synthase and sesquiterpene cyclase in cotton suspension-cultured cells treated with fungal elicitors, Molecular Pant-Microbe Interactions, 1999, 12(12): 1095.

  17. 17.

    Angela, M., Montserrat, A., David, M. et al., Overexpression ofArabidopsis thaliana farnesyl diphosphate synthase (FPS1S) in transgenicArabidopsis induces a cell death/senescence-like response and reduced cytokinin levels, The Plant Journal, 2002, 30(2): 123.

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Correspondence to Hechun Ye.

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Zhao, Y., Ye, H., Li, G. et al. Cloning and enzymology analysis of farnesyl pyrophosphate synthase gene from a superior strain ofArtemisia annua L.. Chin.Sci.Bull. 48, 63–67 (2003). https://doi.org/10.1007/BF03183336

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  • artemisinin
  • farnesyl pyrophosphate synthase (FPS)