Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Molecular tagging of a genic male-sterile gene in rice

  • 18 Accesses


The sterility of Pingxiang male-sterile rice (Pms), possibly derided from a spontaneous mutation in Pingxiang fertile rice (Pmf), was previously reported to be controlled by a single dominant nuclear gene. It can be restored to fertility either by a dominant epistatic gene or by higher temperature treatment at the early stage of inflorescence development. In order to tag the genic male-sterile gene, Pms, Pmf and Ce 64, a cytoplasmic male-sterile restoring line without the epistatic gene for Pms, were used to construct mapping populations. Two segregation populations, “(Pms/Ce 64) F1s (sterile plant)//Pmf” F1 and “Pms//(Pmf/Ce 64) Fl” F1, were simultaneously developed. Subsequently, the genic malesterile gene was mapped between a simple sequence length polymorphism marker, RM228, and a restriction fragment length polymorphism marker, G2155, with distances of 14.9 and 2.6 cM, respectively. The tagged dominant genic male-sterile gene is temporarily designated Ms-p.

This is a preview of subscription content, log in to check access.


  1. 1.

    Zhang, Q. F., Shen, P., An RFLP-based genetic analysis of photoperiod-sensitive male-sterility in rice, Rice Genetics Newsletter, 1993, 10: 94.

  2. 2.

    Oka, H. I., Kush, G. S., Rice Genetics Newsletter, 1998, 15.

  3. 3.

    Li, S. G., Zhou, K. D., Zhu, L. H., Genetics analysis on and tagging a thermo-sensitive dominant male-sterile gene in rice, Chinese Science Bulletin (in Chinese), 1999, 44(9): 955.

  4. 4.

    Yan, N., A., Zhang, J., C., Zhu, C. et al., The preminary evaluation of a dominant male-sterile gene in rice, Acta Agronomica Sinica, 1989, 15(2): 173.

  5. 5.

    McCouch, S. R., Kochert, G., Yu, Z. H., Molecular mapping of rice chromosome, Theor. Appl. Genet., 1988, 76: 148.

  6. 6.

    Zhu, L. H., Xu, J. C., Chen, Y. et al., Tagging a new blast resistance gene in rice by using molecular markers, Science in China, Ser. B, (in Chinese), 1994, 24(10): 1048.

  7. 7.

    Alkagi, H., Yokozeki, Y., Inagaki, A. et al., Microsatellite DNA markers for rice chromosomes, Theor. Appl. Genet., 1996, 93: 1071.

  8. 8.

    Wu, K. S., Tanksley, S. D., Abundance polymorphism and genetic mapping of microsatellites in rice, Mol. Gen. Genet., 1993, 241: 225.

  9. 9.

    Mathilde, A. C., Therea, M. F., Yong, G. C. et al., Saturated moChinese Science Bulletin Vol. 46 No. 1 January 2001 lecular map of the rice genome based on an interspecific backcross poputation, Genetics, 1994, 133: 1251.

  10. 10.

    Yoshiaki, H., Masahiro, Y., Ayahiko, S. et al., A high-density rice genetic linkage map with 2275 markers using a single F2 population, Genetics, 1998, 145: 479.

  11. 11.

    Lander, E. S., Green, P., Abrahamson, J. et al., MAPMARKER: an interactive computer for constructing primary genetics linkage maps of experimental and natural poplations, Genetics, 1987, 1: 174.

  12. 12.

    He, H. H., Liu, Y. B., Cai, Y. H. et al., Inheritance of dominant genic male sterility and its restoration in rice, Chinese Journal of Rice Science, 1999, 13(3): 143.

  13. 13.

    Sasakuma, J., Maan, S. S., EMS-induced male sterile mutants in euplasmic common wheat, Crop Science, 1978, 18: 850.

  14. 14.

    Virmani, S. S., Heterosis and Hybrid Rice Breeding, Berlin: Springer-Verlag, 1994.

Download references

Author information

Correspondence to Shigui Li or Lihuang Zhu.

About this article

Cite this article

Chen, X., Li, S., Wang, W. et al. Molecular tagging of a genic male-sterile gene in rice. Chin.Sci.Bull. 46, 66–69 (2001).

Download citation


  • rice
  • genic male-sterility
  • molecular mapping
  • molecular marker
  • gene localization