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Effect of indomethacin on testicular hypertrophy in hemiorchidectomized immature rats


The effect of indomethacin, an inhibitor of prostaglandin synthesis, on testicular hypertrophy has been studied in immature rats following hemiorchidectomy. Hemiorchidectomy was performed on postnatal day 21 and the weight of the excised testis was recorded. On postnatal day 27, the animals were divided into two groups. Group 1 received saline (0·1 ml) and group 2 received indomethacin orally at 2 mg/kg/day. All animals were treated daily for 15 days. On completion of the treatments, the animals were sacrificed by ether administration. The final body weight and the weight of the remaining testis were recorded. Blood samples were collected by cardiac puncture for testosterone assay. The testes were kept in formol saline. Subsequently, they were prepared for quantitative histological study. Indomethacin significantly decreased the mean diameters of the seminiferous tubules. Cell counts indicated that there was a significant decrease in the mean numbers of spermatogonia, primary spermatocytes and spermatids. The testicular weight gain and the mean testosterone concentrations showed a nonsignificant decrease in treated animals. It is concluded that indomethacin exerts inhibitory action on spermatogenesis in hemiorchidectomized immature rats.

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Ayoub, R.S., Yousif, W.H. Effect of indomethacin on testicular hypertrophy in hemiorchidectomized immature rats. Proc. Indian Acad. Sci. (Anim. Sci.) 98, 419–423 (1989).

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  • Indomethacin
  • testicular hypertrophy
  • testosterone
  • hemiorchidectomy