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Molecular cloning and characterization of bile salt hydrolase inLactobacillus casei Zhang

  • Food Microbiology
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Abstract

Bile salt hydrolase (BSH) is one of the genes relevant to bile tolerance. Biochemical assay showed thatLactobacillus casei Zhang was able to deconjugate sodium taurocholate during growth. To explore the possible relationship between BSH activity and bile tolerant ability, we cloned and determined full-length DNA sequence of the BSH gene inL. casei Zhang, and monitored its expression pattern under the stress of bile salts. The DNA sequence consists of 1181 nucleotides; the putative protein includes 338 amino acids. The sequences alignment and homology studies illustrated a great diversity between species. Further real-time PCR analysis revealed that expression of the BSH gene was up-regulated when there presented bile salts in the growth medium.

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Authors and Affiliations

  1. Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, 010018, Huhhot, P. R. China

    Wen Yi Zhang, Ri Na Wu, Zhi Hong Sun, Tian Song Sun & He Ping Zhang

  2. School of Agriculture and biology Shanghai Jiao Tong University, 200040, Shanghai, P. R. China

    He Meng

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  1. Wen Yi Zhang
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  2. Ri Na Wu
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  3. Zhi Hong Sun
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  4. Tian Song Sun
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  5. He Meng
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  6. He Ping Zhang
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Correspondence to He Meng or He Ping Zhang.

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Zhang, W.Y., Wu, R.N., Sun, Z.H. et al. Molecular cloning and characterization of bile salt hydrolase inLactobacillus casei Zhang. Ann. Microbiol. 59, 721–726 (2009). https://doi.org/10.1007/BF03179214

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  • DOI: https://doi.org/10.1007/BF03179214

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