Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Properties of lactate dehydrogenase isozymes inRana hexadactyla, Rana tigrina andRana cyanophlyctis

  • 15 Accesses

  • 1 Citations


Lactate dehydrogenase isozymes in three species of genusRana demonstrate species specific and tissue specific patterns in their electrophoretic mobility and band pattern. The activity levels of the enzyme, the levels of lactic acid and pyruvic acid and theKm (lactate) suggest that the enzyme in muscle is less efficient as lactate oxidase when compared to that of heart. Heat inactivation studies suggest that the enzyme has some adaptive significance to the habitat preferences of the species.

This is a preview of subscription content, log in to check access.


  1. Adams M J, Buchner M, Chandrashekhar K, Ford G C, Hachkert M L, Liljar A, Rossman M G, Smiley I E, Allison W S, Everse J, Kaplan N O and Taylor S 1973 Structure-function relationships in lactate dehydrogenase;Proc. Natl. Acad. Sci. USA. 70 1968–1972

  2. Alcorn S and Maisel H 1967The ontogeny of LDH in the chick cornea;J. Exp. Zool. 165 309–316

  3. Bernstein L, Kerrigan M and Maisel H 1966 Lactate dehydrogenase isozymes in lens and cornea;Exp. Eye Res. 5 309–314

  4. Blanco A and Zinkham W A 1963 Lactate dehydrogenase in human testes;Science 139 601–602

  5. Daniel J C 1972 Field guide to the amphibians of Western India;J. Bombay Nat. Hist. Soc. 72(2) 506–522

  6. Dessauer H C 1974 Biochemical and immunological evidence of relationships in amphibia and reptilia; inBiochemical and immunological taxonomy of animals (ed. C A Wright) (New York: Academic Press) pp 177–242

  7. Everse J and Kaplan N O 1973 Lactate dehydrogenase; structure and function;Adv. Enzymol. 37 61–133

  8. Everse J and Kaplan N O 1975 Mechanisms of action and biological functions of various dehydrogenase isozymes; inIsozymes-II physiological function (ed. C L Markert) (London: Academic Press) pp 39–44

  9. E C Apparatus corporation, Philadelphia (USA) Technical Bulletin Nos. 134, 135 and 144.

  10. Friedman and Haugen 1942 inHawk’s physiological chemistry 14th ed. (ed. B L Oser) (New Delhi: Tata McGraw-Hill) p. 1108

  11. Goldberg E 1965 Lactate dehydrogenase in trout: Evidence for a third subunit;Science 148 391–392

  12. Goldberg E and Wuntch T 1967 Electrophoretic and kinetic properties ofRana pipiens LDH isozymes;J. Exp. Zool. 165 101–110

  13. Huckabee W E 1961 inHawk’s physiological chemistry 14th edition (ed. B L Oser) (New Delhi: Tata McGraw-Hill) p. 1103

  14. Lowry O H, Rosenbrough N J, Farr A L and Randall R J 1951 Protein measurement with the folin phenol reagent;J. Biol. Chem. 193 265–273

  15. Markert C L 1967 in 7th Int. Congr. Biochem. Tokyo 4 828 (as cited by Masters and Holmes 1975)

  16. Markert C L 1968Ann. N.Y. Acad. Sci. 103 915 (as cited by Masters and Holmes 1975)

  17. Markert C L 1975 Biology of Isozymes; inIsozymes I molecular structure (ed. C L Markert) (London: Academic Press) pp 1–10

  18. Markert C L and Faulhauber I 1965 Lactate dehydrogenase isozyme patterns of fish;J. Exp. Zool. 159 319–332

  19. Markert C L, Shaklee J B and Whitt G S 1975 Evolution of gene;Science 189 102–114

  20. Masters C J and Holmes R S 1975 Isoenzymes and Phylogeny; inHaemoglobin, isoenzymes and tissue differentiation (Amsterdam: North Holland Publishing Co. and New York: American Elsevier) pp 61–128

  21. Moyer F H, Speaker C B and Wright D A 1968 Characteristics of lactate dehydrogenase levels in amphibians;Ann. N.Y. Acad. Sci. 151 650–660

  22. Nachlas M M, Margeulius S P and Selligman A M 1960 A colorimetric method for the estimation of succinate dehydrogenase;J. Biol. Chem. 235 499–503

  23. Nadal-Ginard B and Markert C L 1975 Use of affinity chromatography for the purification of lactate dehydrogenase and for assessing the homology and function of theA andB subunits; inIsozymes-II physiological function (ed. C L Markert) (London: Academic Press) pp 45–68

  24. Ogihara M 1975 Correlation between lactate dehydrogenase Isozymes of the livers and dietary environments in mammals; inIsozymes-II physiological functions (ed. C L Markert) (London: Academic Press) pp 157–170

  25. Reddanna P and Govindappa S 1978 Effect ofin vivo muscular stimulations III. Some aspects of carbohydrate metabolism of cardiac tissue;Curr. Sci. 47 531–533

  26. Salthe S N and Nevo E 1969 Geographic variation of lactate dehydrogenase in the cricket frogAcris crepitans;Biochem. Genet. 3 335–341

  27. Schmiel V C and Guttman S I 1974 An electrophoretic analysis of the lens and muscle proteins;Am Midland Natur. 91 148–159

  28. Schwantes M L 1973 Lactate dehydrogenase isozyme patterns in thirteen species of Snakes;J. Exp. Zool. 185 311–316

  29. Shaklee J B 1975 The role of subunit interactions in the genesis of non-binomial lactate dehydrogenase isozyme distribution; inIsozymes I Molecular structure (ed. C L Markert) (London: Academic Press) pp. 101–118

  30. Shaw C R 1969 Isozymes: Classification, frequency and significance;Int. Rev. Cytol. 25 297–332

  31. Shaw C R and Barto E 1963 Genetic evidence for the subunit structure of lactate dehydrogenase isozymes;Proc. Natl. Acad. Sci. USA. 50 211–214

  32. Ushakov B 1964 Thermostability of cells and proteins of poikilotherms and its significance in specification;Physiol. Rev. 44 518–560

  33. Vesell E S 1975 Medical uses of Isozymes; inIsozymes II physiological function (ed. C L Markert) (London: Academic Press) pp 1–28

  34. Whitt G S, Childers W F and Wheat T E 1971 The inheritance of tissue specific lactate dehydrogenase isozymes in inter specific bass (Micropterus) hybrids;Biochem. Genet. 5 257–273

  35. Wilson A C, Kaplan N O, Levine L, Pesce A, Reichlin M and Allison W S 1964 Evolution of lactic dehydrogenases;Fed. Proc. 23 1258–1266

  36. Wright D A and Moyer F H 1966 Parental influences on lactate dehydrogenase in the early development of hybrid frogs in the genusRana;J. Exp. Zool. 163 1–16

  37. Wright D A and Moyer F H 1973 Immunochemistry of frog lactate dehydrogenase and the subunit homologies of amphibian LDH isozymes;Comp. Biochem. Physiol. B44 1011–1016.

Download references

Author information

Correspondence to V Lakshmipathi.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lakshmipathi, V., Swami, K.S. & Ramana Rao, K.V. Properties of lactate dehydrogenase isozymes inRana hexadactyla, Rana tigrina andRana cyanophlyctis . Proc. Indian Acad. Sci. (Anim. Sci.) 89, 53–67 (1980). https://doi.org/10.1007/BF03179144

Download citation


  • Rana hexadactyla
  • Rana tigrina
  • Rana cyanophlyctis
  • lactate dehydrogenase isozymes