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Differential expression of rice lipid transfer protein gene(LTP) classes in response to abscisic acid, salt, salicylic acid, and the fungal pathogenMagnaporthe grisea

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Rice lipid transfer protein(LTP) genes belong to a complex multigene family and are differentially regulated. The organ specificity of expression for the different classes of riceLTP gene Subfamily I was investigated here.LTPC1 (Class I) was highly expressed in flowers and stems, whileLTPC2 (Class II) showed higher expression in leaves and stems.LTPC3 (Class III) showed higher expression in stems, whereas corresponding mRNA was barely detected in leaves. Treatment of 3-wk-old plants with ABA induced the expression ofLTPC1 andLTPC3 after 1 d. Expression of all threeLTP isoforms was induced in response to salicylic acid. Because some of these genes are reportedly induced by pathogens, we also investigated whether they might be responsive to infection byMagnaporthe grisea. Transcripts ofLTPC1 started to accumulate in the leaves at 8 h after incompatible inoculation, reaching a maximum level at 48 h. In contrast, the response was much slower and weaker after compatible inoculation.

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Literature cited

  1. Arondel V, Vergnolle C, Cantrel C, Kader J-C (2000) Lipid transfer proteins are encoded by a small multigene family inArabidopsis thaliana. Plant Sci159: 1–12

  2. Blein JP, Coutos-Thevenot P, Marion D, Ponchet M (2002) From elicitins to lipid-transfer proteins: A new insight in cell signalling involved in plant defence mechanisms. Trends Plant Sci7: 293–296

  3. Blilou I, Ocampo JA, Garcia-Carrido JM (2000) Induction ofLtp (lipid transfer protein) andPal (phenylalanine ammonia-lyase) gene expression in rice roots colonized by the arbuscular mycorrhizal fungus Glomusmosseae. J Exp Bot51: 1969–1977

  4. Buhot N, Douliez JP, Jacquemard A, Marion D, Tran V, Maume BF, Milat ML, Ponchet M, Mikes V, Kader JC, Blein JP (2001) A lipid transfer protein binds to a receptor involved in the control of plant defence responses. FEBS Lett509: 27–30

  5. Cameron KD, Teece MA, Smart LB (2006) Increased accumulation of cuticular wax and expression of lipid transfer protein in response to periodic drying events in leaves of tree tobacco. Plant Physiol140: 176–183

  6. Garcia-Garrido JM, Menossi M, Puigdomenech P, Martinez-Izquierdo JA, Delseny M (1998) Characterization of a gene encoding an abscisic acid-inducible type-2 lipid transfer protein from rice. FEBS Lett428: 193–199

  7. Garcia-Olmedo F, Molina A, Segura A, Moreno M (1995) The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol3: 72–74

  8. Guiderdoni E, Cordero MJ, Vignols F, Garcia-Garrido JM, Lescot M, Tharreau D, Meynard D, Ferriere N, Notteghem JL, Delseny M (2002) Inducibility by pathogen attack and developmental regulation of the riceLtp1 gene. Plant Mol Biol49: 683–699

  9. Hong ST, Lee MY, Kim SR (2001) Molecular cloning of the anther-preferential nonspecific lipid transfer protein cDNA in hot pepper by mRNA differential display. Mol Cells11: 241–249

  10. Kader JC (1996) Lipid-transfer proteins in plants. Annu Rev Plant Physiol Plant Mol Biol47: 627–654

  11. Kristensen AK, BrunstedtJ, Nielsen KK, Roepstorff R Mikkelsen JD (2000) Characterization of a new antifungal non-specific lipid transfer protein (nsLTP) from sugar beet leaves. Plant Sci155: 31–40

  12. Maldonado AM, Doerner P, Dixon RA, Lamb CJ, Cameron RK (2002) A putative lipid transfer protein involved in systemic resistance signalling inArabidopsis. Nature419: 399–403

  13. Park CJ, Shin R, Park JM, Lee GJ, You JS, Paek KH (2002) Induction of pepper cDNA encoding a lipid transfer protein during the resistance response to tobacco mosaic virus. Plant Mol Biol48: 243–254

  14. Pyee J, Yu H, Kolattukudy PE (1994) Identification of a lipid transfer protein as the major protein in the surface wax of broccoli(Brassica oleracea) leaves. Arch Biochem Biophys311: 460–468

  15. Raskin I (1992) Role of salicylic acid in plants. Annu Rev Plant Physiol Plant Mol Biol43: 439–463

  16. Roy-Barman S, Sautter C, Chattoo BB (2006) Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses. Transgenic Res15: 435–446

  17. Segura A, Moreno M, Carcia-Olmedo F (1993) Purification and antipathogenic activity of lipid transfer proteins (LTPs) from the leavesof Arabidopsis and spinach. FEBS Lett332: 243–246

  18. Shon YC, Lee BH, Kang KY, Lee JJ (2005) Effects of NaCl stress on germination, antioxidant response and proline content in two rice cultivars. J Plant Biol48: 201–208

  19. Vignols F, Lund C, Pammi S, Tremousaygue D, Grellet F, Kader JC, Puigdomenech P, Delseny M (1994) Characterization of a rice gene coding for a lipid transfer protein. Gene142: 265–270

  20. Vignols F, Wigger M, Garcia-Garrido JM, Grellet F, Kader JC, Delseny M (1997) Rice lipid transfer protein (LTP) genes belong to a complex multigene family and are differentially regulated. Gene195: 177–186

  21. Yubero-Serrano EM, Moyano E, Medina-Escobar N, Munoz-Blanco J, Caballero JL (2003) Identification of a strawberry gene encoding a non-specific lipid transfer protein that responds to ABA, wounding and cold stress. J Exp Bot54: 1865–1877

  22. Wu G, Robertson AJ, Liu X, Zheng P, Wilen RW, Nesbitt NT, Gusta LV (2004) A lipid transfer protein geneBG-14 is differentially regulated by abiotic stress, ABA, anisomycin, and sphingosine in bromegrass(Bromus inermis). J Plant Physiol161: 449–458

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Correspondence to Tae Hyun Kim or Moon Chul Kim or Jong Ho Park or Seong Sook Han or Byung Ryun Kim or Byoung Yong Moon or Mi Chung Suh or Sung Ho Cho.

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Kim, T.H., Kim, M.C., Park, J.H. et al. Differential expression of rice lipid transfer protein gene(LTP) classes in response to abscisic acid, salt, salicylic acid, and the fungal pathogenMagnaporthe grisea . J. Plant Biol. 49, 371 (2006). https://doi.org/10.1007/BF03178814

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  • lipid transfer protein
  • Magnaporthe grisea
  • pathogen
  • rice
  • stress