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Annals of Microbiology

, 59:69 | Cite as

Identification of yeast population dynamics of spontaneous fermentation in Beijing wine region, China

  • Huihui Sun
  • Huiqin Ma
  • Meiling Hao
  • Isak S. Pretorius
  • Shangwu ChenEmail author
Food Microbiology Original Articles

Abstract

The aim of this study was (i) to investigate changes occurring in the yeast population profile during spontaneous fermentation of grape juice; (ii) to assess the proliferation of commercial yeast starter culture strains in vineyards; and (iii) to identify indigenous wine strains for future development of starter strains that better reflect the yeast biodiversity of China’s grape-growing regions. To achieve this, yeasts were isolated at four different stages during fermentation of both hand-pressed and winery-sourced must samples ofVitis vinifera L. cv. Roussanne and Merlot. A total of 1600 yeast colonies were isolated and then grouped according to macroscopic and microscopic characteristics. A selection of 291 colonies from the different groups was subjected to species identification using the internal transcribed spacer regions of the 5.8S rRNA gene (ITS1-5.8S-ITS2 region) and the inter-delta (δ) sequence of the 26S rRNA D1/D2 region. In addition, 104Saccharomyces cerevisiae colonies were subjected to strain identification. Twelve species belonging to nine different genera were found amongst the isolates. During the early stages of fermentation, it was found thatHanseniaspora uvarum andCandida stellata numerically dominated the four to six yeast species present, including a region-specific yeast,Sporobolomyces beijingensis. TwoS. cerevisiae strains were isolated from the final stage of fermentation. These two indigenous strains, which were found to be different from the nine commercial yeast strains previously used as starter cultures in this particular Beijing-based winery, might possess potentially important region-specific oenological characteristics. This study provides the first essential step towards the preservation and exploitation of the hidden oenological potential of the untapped wealth of yeast biodiversity in China’s wine-producing regions.

Key words

Beijing wine-producing region spontaneous wine fermentation yeast population profiles 

References

  1. Agnolucci M., Scarano S., Santoro S., Sassano C., Toffanin A., Nuti M. (2007). Genetic and phenotypic diversity of autochthonousSaccharomyces spp. strains associated to natural fermentation of ‘Malvasia delle Lipari’. Lett. Appl. Microbiol., 45: 657–662.CrossRefPubMedGoogle Scholar
  2. Beltran G., Torija M. J., Novo M., Ferrer N., Poblet M., Guillamon J.M., Rozes N., Mas A. (2002). Analysis of yeast populations during alcoholic fermentation: a six year follow-up study. Syst. Appl. Microbiol., 25: 287–293.CrossRefPubMedGoogle Scholar
  3. Combina M., Elia A., Mercado L., Catania C., Ganga A., Martinez C. (2005). Dynamics of indigenous yeast populations during spontaneous fermentation of wines from Mendoza, Argentina. Int. J. Food Microbiol., 99: 237–243.CrossRefPubMedGoogle Scholar
  4. Constanti M., Poblet M., Arola L., Mas A., Guillamón J.M. (1997). Analysis of yeast populations during alcoholic fermentation in a newly established winery. Am. J. Enol. Vitic. 48: 339–344.Google Scholar
  5. De La Torre M.J., Millan M.C., Perez-Juan P., Morales J., Ortega J.M. (1999). Indigenous yeasts associated with twoVitis vinifera grape varieties cultured in southern Spain. Microbios, 100: 27–40.Google Scholar
  6. Delneri D., Colson I., Grammenoudi S., Roberts I.N., Louis E.J., Oliver S.G. (2003). Engineering evolution to study speciation in yeasts. Nature, 422: 68–72.CrossRefPubMedGoogle Scholar
  7. Demuyter C., Lollier M., Legras J.L., Le Jeune C. (2004). Predominance ofSaccharomyces uvarum during spontaneous alcoholic fermentation, for three consecutive years, in an Alsatian winery. J. Appl. Microbiol., 97: 1140–1148.CrossRefPubMedGoogle Scholar
  8. Di Maro E., Ercolini D., Coppola S. (2007). Yeast dynamics during spontaneous wine fermentation of the Catalanesca grape. Int. J. Food Microbiol., 117: 201–210.CrossRefPubMedGoogle Scholar
  9. Domizio P., Lencioni L., Ciani M., Di Blasi S., Pontemolesi C., Sabatelli M.P. (2007). Spontaneous and inoculated yeast populations dynamics and their effect on organoleptic characters of Vinsanto wine under different process conditions. Int. J. Food Microbiol., 115: 281–289.CrossRefPubMedGoogle Scholar
  10. Egli C.M., Edinger W.D., Mitrakul C.M., Henick-Kling T. (1998). Dynamics of indigeous and inoculated yeast populations and their effect on the sensory character of Riesling and Chardonnay wines. J. Appl. Microbiol., 85: 779–789.CrossRefPubMedGoogle Scholar
  11. Esteve-Zarzoso B., Peris-Toran M., Garcia-Maiquez E., Uruburu F., Querol A. (2001). Yeast population dynamics during the fermentation and biological aging of sherry wines. Appl. Environ. Microbiol., 67: 2056–2061.CrossRefPubMedGoogle Scholar
  12. Fell J.W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. (2000). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol., 50 Pt 3: 1351–1371.PubMedGoogle Scholar
  13. Fleet G.H. (2003). Yeast interactions and wine flavour. Int. J. Food Microbiol., 86: 11–22.CrossRefPubMedGoogle Scholar
  14. Fleet G.H., Lafon-Lafourcade S., Ribereau-Gayon P. (1984). Evolution of yeasts and lactic acid bacteria during fermentation and storage of Bordeaux wines. Appl. Environ. Microbiol., 48: 1034–1038.PubMedGoogle Scholar
  15. Guillamon J.M., Sabate J., Barrio E., Cano J., Querol A. (1998). Rapid identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer (ITS) region. Arch. Microbiol., 169: 387–392.CrossRefPubMedGoogle Scholar
  16. Gutiérrez A.R., Santamaría P., Epifanio S., Garijo P., López R. (1999). Ecology of spontaneous fermentation in one winery during 5 consecutive years. Lett. Appl. Microbiol., 29: 411–415.CrossRefGoogle Scholar
  17. Hierro N., Gonzalez A., Mas A., Guillamon J.M. (2006). Diversity and evolution of non-Saccharomyces yeast populations during wine fermentation: effect of grape ripeness and cold maceration. FEMS Yeast Res., 6: 102–111.CrossRefPubMedGoogle Scholar
  18. Jolly N.P., Augustyn O.P.H., Pretorius I.S. (2003a). The occurrence of non-Saccharomyces yeast species over three vintages on four vineyards and grape musts from four production regions of the Western Cape, South Africa. S. Afr. J. Enol. Vitic., 24: 35–42.Google Scholar
  19. Jolly N.P., Augustyn O.P.H., Pretorius I.S. (2003b). The effect of non-Saccharomyces yeasts on fermentation and wine quality. S. Afr. J. Enol. Vitic., 24: 55–62.Google Scholar
  20. Jolly N.P., Augustyn O.P.H., Pretorius I.S. (2003c). The use ofCandida pulcherrima in combination withSaccharomyces cerevisiae for the production of Chenin blanc wine. S. Afr. J. Enol. Vitic., 24: 63–69.Google Scholar
  21. Jolly N.P., Augustyn O.P.H., Pretorius I.S. (2006). The role and use of non-Saccharomyces yeasts in wine production. S. Afr. J. Enol. Vitic., 27: 15–39.Google Scholar
  22. Khan W., van der Westhuizen T.J., Augustyn O.P.H., Pretorius I.S. (2000). Geographic distribution and evaluation ofSaccharomyces cerevisiae strains isolated from vineyards in the warm, inland regions of the Western Cape in South Africa. S. Afr. J. Enol. Vitic., 21: 17–31.Google Scholar
  23. Lambrechts M.G., Pretorius I.S. (2000). Yeast and its importance to wine aroma — a review. S. Afr. J. Enol. Vitic., 21: 97–129.Google Scholar
  24. Legras J.L., Karst F. (2003). Optimisation of interdelta analysis forSaccharomyces cerevisiae strain characterisation. FEMS Microbiol. Lett, 221: 249–255.CrossRefPubMedGoogle Scholar
  25. Longo E., Cansado J., Agrelo D., Villa T.G. (1991). effect of climatic conditions on yeast diversity in grape musts from Northwest Spain. Am. J. Enol. Vitic., 42: 141–144.Google Scholar
  26. Lopes C.A., van Broock M., Querol A., Caballero A.C. (2002).Saccharomyces cerevisiae wine yeast populations in a cold region in Argentinean Patagonia. A study at different fermentation scales. J. Appl. Microbiol., 93: 608–615.CrossRefPubMedGoogle Scholar
  27. Makimura K., Murayama S. Y., Yamaguchi H. (1994). Detection of a wide range of medically important fungi by the polymerase chain reaction. J. Med. Microbiol., 40: 358–364.CrossRefPubMedGoogle Scholar
  28. Mannazzu I., Simonetti E., Marinangeli P., Guerra E., Budroni M., Thangavelu M., Clementi F. (2002). SED1 gene length and sequence polymorphisms in feral strains ofSaccharomyces cerevisiae. Appl. Environ. Microbiol., 68: 5437–5444.CrossRefPubMedGoogle Scholar
  29. Martinez C., Cosgaya P., Vasquez C., Gac S., Ganga A. (2007). High degree of correlation between molecular polymorphism and geographic origin of wine yeast strains. J. Appl. Microbiol., 103:2185–2195.CrossRefPubMedGoogle Scholar
  30. Masneuf-Pomarede I., Le Jeune C., Durrens P., Lollier M., Aigle M., Dubourdieu D. (2007). Molecular typing of wine yeast strainsSaccharomyces bayanus var.uvarum using microsatellite markers. Syst. Appl. Microbiol., 30: 75–82.CrossRefPubMedGoogle Scholar
  31. Masoud W., Cesar L.B., Jespersen L., Jakobsen M. (2004). Yeast involved in fermentation of Coffea arabica in East Africa determined by genotyping and by direct denaturating gradient gel electrophoresis. Yeast, 21: 549–556.CrossRefPubMedGoogle Scholar
  32. Mills D.A., Johannsen E.A., Cocolin L. (2002). Yeast diversity and persistence in botrytis-affected wine fermentations. Appl. Environ. Microbiol., 68: 4884–4893.CrossRefPubMedGoogle Scholar
  33. Mortimer R., Polsinelli M. (1999). On the origins of wine yeast. Res. Microbiol., 150: 199–204.CrossRefPubMedGoogle Scholar
  34. Ness F., Lavallee F., Dubourdieu D., Aigle M., Dulau L. (1993). Identification of yeast strains using the polymerase chain reaction. J. Sci. Food Agri., 62: 89–94.CrossRefGoogle Scholar
  35. Nisiotou A.A., Spiropoulos A.E., Nychas G.J. (2007). Yeast community structures and dynamics in healthy and Botrytisaffected grape must fermentations. Appl. Environ. Microbiol., 73: 6705–6713.CrossRefPubMedGoogle Scholar
  36. Pretorius I.S. (2000). Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking. Yeast, 16: 675–729.CrossRefPubMedGoogle Scholar
  37. Pretorius I.S., Van der Westhuizen T.J., Augustyn O.P.H. (1999). Yeast biodiversity in vineyards and wineries and its importance to the South African wine industry. S. Afr. J. Enol. Vitic., 20: 61–74.Google Scholar
  38. Querol A., Jiménez M., Huerta T. (1990). Microbiological and enological parameters during fermentation of musts from poor and normal grape-harvest in the region of Alicante, Spain. J. Food Sci., 55: 1603–1606.CrossRefGoogle Scholar
  39. Querol A., Fernandez-Espinar M.T., del Olmo M., Barrio E. (2003). Adaptive evolution of wine yeast. Int. J. Food Microbiol., 86: 3–10.CrossRefPubMedGoogle Scholar
  40. Renouf V., Falcou M., Miot-Sertier C., Perello M.C., DeRevel G., Lonvaud-Funel A. (2006). Interactions betweenBrettanomyces bruxellensis and other yeast species during the initial stages of winemaking. J. Appl. Microbiol., 100: 1208–1219.CrossRefPubMedGoogle Scholar
  41. Schütz M., Gafner J. (1994). Dynamics of the yeast strain population during spontaneous alcoholic fermentation determined by CHEF gel electrophoresis. Lett. Appl. Microbiol., 19: 253–257.CrossRefGoogle Scholar
  42. Schuller D., Pereira L., Alves H., Cambon B., Dequin S., Casal M. (2007). Genetic characterization of commercialSaccharomyces cerevisiae isolates recovered from vineyard environments. Yeast, 24: 625–636.CrossRefPubMedGoogle Scholar
  43. Sipiczki M., Romano P., Capece A., Paraggio M. (2004). Genetic segregation of naturalSaccharomyces cerevisiae strains derived from spontaneous fermentation of Aglianico wine. J. Appl. Microbiol., 96: 1169–1175.CrossRefPubMedGoogle Scholar
  44. Valero E., Schuller D., Cambon B., Casal M., Dequin S. (2005). Dissemination and survival of commercial wine yeast in the vineyard: a large-scale, three-years study. FEMS Yeast Res., 5: 959–969.CrossRefPubMedGoogle Scholar
  45. Van der Westhuizen T.J., Augustyn O.P.H., Pretorius I.S. (2000a). Geographic distribution of indigenousSaccharomyces cerevisiae strains isolated from vineyards in the coastal regions of the Western Cape in South Africa. S. Afr. J. Enol. Vitic., 21: 3–9.Google Scholar
  46. Van der Westhuizen TJ, Augustyn O.P.H., Khan W., Pretorius I.S. (2000b). Seasonal variation of indigenousSaccharomyces cerevisiae strains isolated from Western Cape vineyards in South Africa. S. Afr. J. Enol. Vitic., 21: 10–16.Google Scholar
  47. van Keulen H., Lindmark D.G., Zeman K.E., Gerlosky W. (2003). Yeasts present during spontaneous fermentation of Lake Erie Chardonnay, Pinot Gris and Riesling. Antonie van Leeuwenhoek, 83: 149–154.CrossRefPubMedGoogle Scholar
  48. Versavaud A., Courcoux P., Roulland C., Dulau L., Hallet J.N. (1995). Genetic diversity and geographical distribution of wildSaccharomyces cerevisiae strains from the wine-producing area of Charentes, France. Appl. Environ. Microbiol., 61: 3521–3529.PubMedGoogle Scholar
  49. Wang Q.M., Bai F.Y. (2004). Four new yeast species of the genusSporobolomyces from plant leaves. FEMS Yeast Res., 4: 579–586.CrossRefPubMedGoogle Scholar
  50. White T.J., Bruns T., Lee S., Taylor J. (1990). Amplification and direct sequencing of fungi ribosomal RNA genes for phylogenetics. In: Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., Eds, PCR Protocols: A Guide To Methods And Applications. Academic Press, San Diego, California, pp. 315–322.Google Scholar
  51. Yap N.A., de Barros Lopes M., Langridge P., Henschke P.A. (2000). The incidence of killer activity of non-Saccharomyces yeasts towards indigenous yeast species of grape must: potential application in wine fermentation. J. Appl. Microbiol., 89: 381–389.CrossRefPubMedGoogle Scholar

Copyright information

© University of Milan and Springer 2009

Authors and Affiliations

  • Huihui Sun
    • 1
  • Huiqin Ma
    • 1
  • Meiling Hao
    • 1
  • Isak S. Pretorius
    • 2
  • Shangwu Chen
    • 3
    Email author
  1. 1.College of Agriculture and BiotechnologyChina Agricultural UniversityBeijingChina
  2. 2.The Australian Wine Research InstituteGlen Osmond (AdelaideAustralia
  3. 3.College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina

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