Annals of Microbiology

, Volume 59, Issue 1, pp 17–23 | Cite as

Occurrence of multiple metal-resistance in bacterial isolates associated with transgenic white poplars (Populus alba L.)

  • Alma BalestrazziEmail author
  • Martina Bonadei
  • Emanuele Quattrini
  • Daniela Carbonera
Ecological and Environmental Microbiology Original Articles


The occurrence of multiple metal-resistance was assessed in two bacterial collections, named Herbicide Resistant Bacteria (HRB) and Nuclease-Producing Bacteria (NPB) respectively, consisting of 15 and 11 isolates obtained from a loamy sand cultivated with transgenic white poplars (Populus alba L., cv ‘Vilafranca’) engineered for herbicide resistance. A third collection of 11 bacterial isolates, named Leaf-Associated Bacteria (LAB), obtained from the leaves of transgenic white poplars expressing theStSy gene for resveratrol production and from untransformed plants was evaluated. Resistance to Cd, Co, Cu, Pb and Zn was tested. As for the HRB collection, nine different phenotypes were monitored, which included tetra-, tri- and double-resistance. Tri- and double-metal resistance occurred also within the NPB and LAB collections. In both cases five different phenotypes were recovered. An additional investigation was carried out on the HRB-1c isolate, resistant to Cd, Co, Pb and Zn, which was previously demonstrated to produce indoleacetic acid, a plant-growth-promoting trait. Colorimetric assays, performed on the cell-depleted medium of HRB-1c liquid cultures grown in presence of heavy metals, confirmed that this trait was not affected. A 19-kb plasmid, possibly involved in the maintenance of the multiple metal-resistant phenotype, was detected in the HRB-1c cells.

Key words

leaf-associated bacteria multiple metal-resistance transgenic poplar 


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Copyright information

© University of Milan and Springer 2009

Authors and Affiliations

  • Alma Balestrazzi
    • 1
    Email author
  • Martina Bonadei
    • 1
  • Emanuele Quattrini
    • 2
  • Daniela Carbonera
    • 1
  1. 1.Dipartimento di Genetica e MicrobiologiaUniversità di PaviaPavia
  2. 2.C.E.T.A.S.Università degli Studi di MilanoTavazzano, LodiItaly

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