Annals of Microbiology

, 59:425 | Cite as

Bacteria flora associated with different body parts of hatchery reared juvenilePenaeus monodon, tanks water and sediment

  • Shahram Shakibazadeh
  • Che Roos Saad
  • Annie Christianus
  • Mohad Salleh Kamarudin
  • Kamaruzaman Sijam
  • Mariana Nor Shamsudin
  • Vasantha Kumari Neela
Ecological and Environmental Microbiology Original Articles
Received:
Accepted:

Abstract

Bacteria flora of intestine and hepatopancreas, body surface and muscles of juvenilePenaeus monodon along with its rearing water and sediment was analyzed. Juvenile shrimp were reared in four tanks in the Hatchery complex, Department of Aquaculture, Faculty of Agriculture, University Putra Malaysia. Water quality parameters were measured every day. Samples were collected aseptically and homogenized before being inoculated in Tryptone Soy agar, Thiosulphate Citrate Bile Salt agar, MacConkey agar andPseudomonas-isolating agar. There was no significant difference between water quality parameters and shrimp body weight of replicate tanks. Total plate count for water and totalVibrio count for rearing water and digestive system were within previous reported ranges. Eight different genera were isolated in which 7 genera were identified. Gram negative bacteria were dominant (72%)Vibrio was the most dominant genera followed byShewanella andBurkholderia. Clavibacter followed byStaphylococcus were the most dominant gram positive bacteria. No coliform bacteria was detected in the shrimp body parts and rearing environment. Incidence ofShewanella in the digestive system was significantly higher than sediment, rearing water and muscles. This may be implied its ability to colonize in the digestive tract of juvenileP. monodon.

Key words

shrimp Penaeus monodon microflora Vibrio Shewanella 
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References

  1. Anderson I.G., Nor Shamsudin M., Nash G. (1989). A preliminary study on the aerobic heterotrophic bacterial flora in giant fresh water prawn,Macrobrachium rosenbergii, hatcheries in Malaysia. Aquaculture, 81: 213–223.CrossRefGoogle Scholar
  2. Bhaskar N., Rudra Setty T.M., Vidya Sagar Reddy G., Manoj Y.B., Anantha C.S., Raghunath B.S., Antony J.M. (1995). Incidence ofSalmonella in cultured shrimpPenaeus monodon. Aquaculture, 138: 257–266.CrossRefGoogle Scholar
  3. Brisou J., Tysset C., de la Roy Y.R., Curcier R. (1965). Marine bacteria especially Micrococcaceae. J. Gen. Microbiol., 41 (1): 23–41.Google Scholar
  4. Buchanan R.E. (1984). Bergey’s Manual of Determinative Bacteriology, Williams and Wilkins Co., Baltimore.Google Scholar
  5. Chahill M.M. (1990). Bacterial flora of fishes: A review. Microb. Ecol., 19: 21–41.CrossRefGoogle Scholar
  6. Colorni A. (1985). A study on the bacterial flora of giant prawn,Macrobrachium rosenbergii, larvae fed withArtemia salina nauplii. Aquaculture, 49: 1–10.CrossRefGoogle Scholar
  7. Costa R., Mermoud I., Koblavi S., Morlet B., Haffner P., Berthe F., Legroumellec M. Grimont P. (1998). Isolation and characterization of bacteria associated with aPenaeus stylirostris disease (Syndrome 93) in New Caledonia. Aquaculture, 164: 297–309.CrossRefGoogle Scholar
  8. Dilmore L.A. (1986). Vibrios of some deep-water invertebrates. FEMS Microbiol. Lett., 35: 221–224.CrossRefGoogle Scholar
  9. Drew W.L., Edelstein M.A.C., Garcia L.S., Roberts G.D. (1986). Bailey and Scott’s Diagnostic Microbiology, C. V. Mosby Co., ST. Louis.Google Scholar
  10. Hameed S. (1993). A study of the aerobic heterotrophic bacterial flora of hatery-reared eggs, larvae and post-larvaeofPenaeus indicus. Aquaculture, 117: 195–204.CrossRefGoogle Scholar
  11. Kennedy B., Venugopal M.N., Karunasagar I., Karunasagar I. (2006). Bacterial flora associated with the giant freshwater prawnMacrobrachium rosenbergii, in the hatchery system. Aquaculture, 261: 1156–1167.CrossRefGoogle Scholar
  12. Leaño E.M., Lavilla-Pitogo C.R., Paner M.G. (1998). Bacterial flora in the hepatopancreas of pond-rearedPenaeus monodon juveniles with luminous vibriosis. Aquaculture, 164: 367–374.CrossRefGoogle Scholar
  13. Li C.C., Yeh S.T., Chen J.C. (2008). The immune respose of white shrimpLitopenaeus vannamei followingVibrio alginolyticus injection. Fish Shellfish Immun., 25 (6): 850–860.Google Scholar
  14. Lightner D.V. (1996). A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp. World Aquaculture Society, Baton Rouge, LA, USA.Google Scholar
  15. MacFaddin J.F. (1980). Biochemical Tests for Identification of Medical Bacteria, Williams and Wilkins, Baltimore.Google Scholar
  16. Moriarty D.J.W. (1998). Control of luminousVibrio species in penaeid aquaculture pond. Aquaculture, 164: 351–358.CrossRefGoogle Scholar
  17. Muroga K., Higashi M., Keitoku H. (1987). The isolation of intestinal microflora of farmed red sea bream (Pagrus major) and black sea bream (Acanthopagrus schlegeli) at larval and juvenile stages. Aquaculture, 65: 79–88.CrossRefGoogle Scholar
  18. Olsson C., Ahrne S., Pettersson B., Molin G. (2004). DNA based classification of food associated Enterobacteriaceae previously identified by biolog GN Microplates. Syst. Appl. Microbiol., 27: 219–228.CrossRefPubMedGoogle Scholar
  19. Otta S.K., Karunasagar I., Karunasagar I. (2001). Bacteriological study of shrimp,Penaeus monodon Fabricius, hatcheries in India. J. Appl. Ichthyol., 17: 59–63.CrossRefGoogle Scholar
  20. Phatarpekar P.V., Kenkre V.D., Sreepada R.A, Desai U.M., Achuthankutty C.T. (2002). Bacterial flora associated with larval rearing of the giant freshwater prawn,Macrobrachium rosenbergii. Aquaculture, 203: 279–291.CrossRefGoogle Scholar
  21. Sung H., Hsu S., Chen C., Ting Y., Chao W. (2001). Relationships between disease outbreak in cultured tiger shrimp (Penaeus monodon) and the composition of Vibrio communities in pond water and shrimp hepatopancreas during cultivation. Aquaculture, 192: 101–110.CrossRefGoogle Scholar
  22. Sung H., Li H., Tsai F., Ting Y., Chaoa W. (1999). Changes in the composition of Vibrio communities in pond water during tiger shrimp (Penaeus monodon) cultivation and in the hepatopancreas of healthy and diseased shrimp. J. Exp. Mar. Biol. Ecol., 236: 261–271.CrossRefGoogle Scholar
  23. Vanderzant C., Nickelson R., Judkins P.W. (1971). Microbial flora of pond-reared brown shrimp (Penaeus aztecus). Appl. Microbiol., 21: 916–921.PubMedGoogle Scholar
  24. Vaseeharan B.R.P. (2003). Abundance of potentially pathogenic micro-organisms inPenaeus monodon larvae rearing systems in India. Microbiol. Res., 158: 299–308.CrossRefPubMedGoogle Scholar
  25. Wang Y., Xu Z., Zhou X., Xia, M. (2005). Bacteria attached to suspended particles in Northern white shrimp (Penaeus vannamei L.) ponds. Aquaculture, 249: 285–290.CrossRefGoogle Scholar
  26. Yasuda K., Kiato T. (1980). Bacterial flora in the digestive tract of prawns,Penaeus japonicus bate. Aquaculture, 19: 229–234.CrossRefGoogle Scholar

Copyright information

© University of Milan and Springer 2009

Authors and Affiliations

  • Shahram Shakibazadeh
    • 1
  • Che Roos Saad
    • 1
  • Annie Christianus
    • 1
  • Mohad Salleh Kamarudin
    • 1
  • Kamaruzaman Sijam
    • 2
  • Mariana Nor Shamsudin
    • 3
  • Vasantha Kumari Neela
    • 3
  1. 1.Department of Aquaculture, Faculty of AgricultureUniversity Putra MalaysiaSerdangMalaysia
  2. 2.Department of Plant ProtectionUniversity Putra MalaysiaSerdangMalaysia
  3. 3.Faculty of Medicine and Health ScienceUniversity Putra MalaysiaSerdangMalaysia

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