Isolation and characterisation of moderately halophilic bacteriumHalomonas ventosae DL7 synthesizing ectoine as compatible solute
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A moderately halophilic Gram-negative bacterium, strain DL7, was isolated from saltern sediment of Dalian, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this isolate belongs to the genusHalomonas. Based on the taxonomic and DNA sequence in addition to high DNA-DNA homologies, we concluded that this strain was similar with the type strain ofHalomonas ventosae. Ectoine, one of the representative compatible solutes, was mainly detected in the cells as a result of1H- and13C-NMR measurements when grown in the presence of wide concentration ranges of NaCl. The results showed that higher amount of ectoine was synthesized in a shorter incubation time compared with those of other strains reported earlier.
Key wordscompatible solute ectoine Halomonas ventosae 16S rRNA sequence
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