Journal of Physiology and Biochemistry

, Volume 60, Issue 2, pp 85–91 | Cite as

Daily ingestion of fermented milk containing Lactobacillus casei DN114001 improves innate defense capacity in healthy middle-aged people

  • M. D. Parra
  • B. E. Martínez de Morentin
  • J. M. Cobo
  • A. Mateos
  • J. A. Martínez
Article

Abstract

Different lactic acid bacteria have often been administered as a dietary means to enhance immune system activity. Based on this statement, the aim of the current work was to test the effects of a Lactobacillus casei DN114001 fermented milk consumption on the immune response capacity in middle-age volunteers. Forty-five healthy volunteers, 24 women and 21 men (aged: 51–58 years), were randomized into two groups to receive three cups per day of a L. casei DN114001 (108–1010 ufc/g) fermented milk (n=23), or placebo (n=22), during an 8-week period. Measurements were performed before (day 0), and after the nutritional intervention (day 56). After the trial, no changes in immune cell proportions were detected, but the probiotic-treated group increased oxidative burst capacity of monocytes (probiotic group: p=0.029; placebo group: p=0.625), as well as NK cells tumoricidal activity (probiotic group: p=0.023; placebo group: p=0.125). Results showed that daily intake of fermented milk containing Lactobacillus casei DN114001 could have a positive effect in modulating the innate immune defense in healthy-middle-age people.

Key words

Lactobacillus casei Ageing Immunity Monocytes NK cells 

Consumo diariode leche fermentada con Lactobacillus casei DN114001 y mejora de la capacidad de defensa innata en personas de mediana edad

Resumen

El consumo de ciertas cepas de bacterias acidolácticas podría ser beneficioso para la actividad del sistema immune. El objetivo del presente trabajo consistió en verificar dichos efectos tras consumo de leche fermentada conteniendo Lactobacillus casei DN114001. En el estudio participaron 45 voluntarios sanos, 23 mujeres y 22 hombres, de edad entre 51 y 58 años. Durante 8 semanas, los sujetos consumieron 3 unidades al día del producto que les fue asignado de manera aleatoria: leche fermentada (n=23) con L. casei DN114001 (108–1010 ufc/g), o leche no fermentada a modo de placebo (n=22). Las determinaciones en muestras sanguíneas se realizaron al inicio (día 0) y al final del ensayo (día 56). Tras el período de intervención nutricional, el grupo que consumió el probiótico incrementó significativamente la capacidad oxidativa de los monocitos (probiótico: p=0.029; placebo: =0.625), y la capacidad tumoricida de las células NK (probiótico: p=0.023; placebo: =0.123). Estos resultados sugieren que el consumo regular de leche fermentada con presencia de Lactobacillus casei DN114001 podría potenciar la inmunidad innata en personas de mediana edad.

Palabras clave

Lactobacillus casei Envejecimiento Inmunidad Monocitos Linfocitos NK 

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References

  1. 1.
    Borruel, N., Casellas, F., Antolin, M., Llopis, M., Carol, M., Espiin, E., Naval, J., Guarner, F. and Malagelada, J. R. (2003): Am. J. Gastroenterol., 98, 865–870.CrossRefPubMedGoogle Scholar
  2. 2.
    Cano, P. G. and Perdigon, G. (2003): J. Dairy Res., 70, 433–440.CrossRefPubMedGoogle Scholar
  3. 3.
    Castle, S. C. (2000): Clin. Infect. Dis., 31, 578–585.CrossRefPubMedGoogle Scholar
  4. 4.
    Effros, R. B. (2003): Mech. Ageing. Dev., 124, 71–77.CrossRefPubMedGoogle Scholar
  5. 5.
    Gauffin, C. P., Agüero, G. and Perdigon, G. (2002): Biocell, 26, 35–48.PubMedGoogle Scholar
  6. 6.
    Gavazzi, G. and Krause, K. H. (2002): Lancet, 2, 659–666.CrossRefGoogle Scholar
  7. 7.
    Gill, H. S., Rutherfurd, K. J., Cross, M. L. and Gopal, P. K. (2001): Am. J. Clin. Nutr., 74, 833–839.PubMedGoogle Scholar
  8. 8.
    Gill, H. S., Rutherfurd, K. J. and Cross M. L. (2001): J. Clin. Immunol., 21, 264–271.CrossRefPubMedGoogle Scholar
  9. 9.
    Goodwin, J. S. (1995): Nutr. Rev., 53, 41–45.CrossRefGoogle Scholar
  10. 10.
    Gorbach, S. L. (2002): Dig. Liver Dis., 34, 2–7.CrossRefGoogle Scholar
  11. 11.
    Gotteland, M., Cruchet, S. and Verbeke, S. (2001): Aliment. Pharmacol. Ther., 15, 11–17.CrossRefPubMedGoogle Scholar
  12. 12.
    Hakim, F..., Flomerfelt, F. A., Boyiadzis, M. and Gress, R. E. (2004): Curr. Opin. Immunol., 16, 151–156.CrossRefPubMedGoogle Scholar
  13. 13.
    Haller, D., Blum, S., Bode, C., Hammes, W. P. and Schiffrin, E. (2000): Infect. Immun., 68, 752–759.CrossRefPubMedGoogle Scholar
  14. 14.
    Isolauri, E., Sütas, Y., Kankaapää, P., Arvilommi, H. and Salminen, S. (2001): Am. J. Clin. Nutr., 73, 444–450.Google Scholar
  15. 15.
    Kemp, F. W., DeCandia, J., Li, W., Bruening, E., Baker, H., Rigassio, D., Bendrich, A. and Bogden, J. D. (2002): Nutr. Res., 22, 45–53.CrossRefGoogle Scholar
  16. 16.
    LeBlanc, J. G., Matar, C., Valdez, J. C., LeBlanc, J. and Perdigon, G. (2002): J. Dairy Sci., 85, 2733–2742.CrossRefPubMedGoogle Scholar
  17. 17.
    Lee, J. W., Shin, J. G., Kim, E. H., Kang, H. E., Yim, I. B., Kim, J. Y., Joo, H. G. and Woo, H. J. (2004): J. Vet. Sci., 5, 41–48.PubMedGoogle Scholar
  18. 18.
    Lopez-Varela, S., Gonzalez-Gross, M. and Marcos, A. (2002): Eur. J. Clin. Nutr., 56, 29–33.CrossRefGoogle Scholar
  19. 19.
    Lord, J. M., Butcher, S., Killampali, V., Lascelles, D. and Salmon, M. (2001): Mech. Ageing. Dev., 122, 1521–1535.CrossRefPubMedGoogle Scholar
  20. 20.
    Moss, G. E. (2004): BMJ, 328, 350–357.CrossRefPubMedGoogle Scholar
  21. 21.
    Murasko, D. M., Bernstein, E. D., Gardner, E. M., Gross, P., Munk, G., Dran, S. and Abrutyn, E. (2002): Exp. Gerontol., 37, 427–439.CrossRefPubMedGoogle Scholar
  22. 22.
    Parra, M. D., Martínez de Morentin, B. E., Cobo, J. M., Mateos, A. and Martínez, J. A. (2004): J. Nutr. Health (in press).Google Scholar
  23. 23.
    Roberfroid, M. B. (2000): Nutrition, 16, 689–691.CrossRefPubMedGoogle Scholar
  24. 24.
    Saris, W. H. M., Antoine, J. M., Brouns, F., Fogelholm, M., Gleeson, M., Hespel, P., Jaukendrup, A. E., Maughan, R. J., Pannemans, D. and Stich, V. (2003): Eur. J. Nutr., 42, 50–95.CrossRefGoogle Scholar
  25. 25.
    Schiffrin, E. J., Brassart, D., Servin, A. L., Rochat, F. and Donnet-Hughes, A. (1997): Am. J. Clin. Nutr., 66, 515–520.Google Scholar
  26. 26.
    Sheih, Y. H., Chiang, B. L., Wang, L. H., Liao, C. K. and Gill, H. S. (2001): J. Am. Coll. Nutr., 20, 149–156.PubMedGoogle Scholar
  27. 27.
    Teitelbaum, L. E. and Walker, W. A. (2002): Annu. Rev. Nutr., 22, 107–138.CrossRefPubMedGoogle Scholar
  28. 28.
    Vaughan, E. E. and Mollet, B. (1999): Nahrung, 43, 148–153.CrossRefPubMedGoogle Scholar
  29. 29.
    Williamson, A. R., Hunt, A. E., Pope, J. F. and Tolman, N. M. (2000): Diabetes Educ., 26, 272–279.CrossRefPubMedGoogle Scholar

Copyright information

© Universidad de Navarra 2004

Authors and Affiliations

  • M. D. Parra
    • 1
  • B. E. Martínez de Morentin
    • 1
  • J. M. Cobo
    • 2
  • A. Mateos
    • 2
  • J. A. Martínez
    • 1
  1. 1.Department of Physiology and NutritionUniversity of NavarraPamplona
  2. 2.Red INDEBarcelonaSpain

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