Broad-band quasi-optical HF-EPR spectroscopy: Application to the study of the ferrous iron center from a rubredoxin mutant

  • A. L. Barra
  • A. K. Hassan
  • A. Janoschka
  • C. L. Schmidt
  • V. Schünemann


High-frequency electron paramagnetic resonance (HF-EPR) allows obtaining detailed information on the anisotropy of integer-spin systems, which are usually silent at conventional EPR frequencies (X- or Q-bands). However, the studies performed so far on integer-spin systems have mostly dealt with synthetic complexes. In this paper, we present first results obtained on a mononuclear high-spin ferrous iron center of a rubredoxin-type protein. This study shows that the broad-band quasi-optical technique of HF-EPR is a suitable approach towards the study of biologically relevant metal centers with large zero-field splittings.


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Copyright information

© Springer 2006

Authors and Affiliations

  • A. L. Barra
    • 1
  • A. K. Hassan
    • 2
  • A. Janoschka
    • 3
  • C. L. Schmidt
    • 4
  • V. Schünemann
    • 3
  1. 1.Grenoble High Magnetic Field LaboratoryCentre National de la Recherche ScientifiqueGrenoble Cedex 9France
  2. 2.Bruker Biospin AGFällandenSwitzerland
  3. 3.Department of PhysicsTechnical University of KaiserslauternKaiserslauternGermany
  4. 4.Institute of Biochemistry, Center for Structural and Cell Biology in MedicineUniversity of LübeckLübeckGermany

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