Broad-band quasi-optical HF-EPR spectroscopy: Application to the study of the ferrous iron center from a rubredoxin mutant

  • A. L. Barra
  • A. K. Hassan
  • A. Janoschka
  • C. L. Schmidt
  • V. Schünemann
Article

Abstract

High-frequency electron paramagnetic resonance (HF-EPR) allows obtaining detailed information on the anisotropy of integer-spin systems, which are usually silent at conventional EPR frequencies (X- or Q-bands). However, the studies performed so far on integer-spin systems have mostly dealt with synthetic complexes. In this paper, we present first results obtained on a mononuclear high-spin ferrous iron center of a rubredoxin-type protein. This study shows that the broad-band quasi-optical technique of HF-EPR is a suitable approach towards the study of biologically relevant metal centers with large zero-field splittings.

References

  1. 1.
    Lebedev Y.S., in: Modern Pulsed and Continuous-Wave Electron Spin Resonance (Kevan L., Bowman M.K., eds.), pp 365–404. New York: Wiley 1990.Google Scholar
  2. 2.
    Fuchs M.R., Prisner T.F., Möbius K.: Rev. Sci. Instrum.70, 3681–3683 (1999)CrossRefADSGoogle Scholar
  3. 3.
    Blok H., Disselhorst J.A.J.M., Orlinskii S.B., Schmidt J.: J. Magn. Reson.166, 92–99 (2004)CrossRefADSGoogle Scholar
  4. 4.
    van Tol H., Brunel L.C.: Rev. Sci. Instrum.76, 074101 (2005)CrossRefADSGoogle Scholar
  5. 5.
    Lynch B., Earle K.A., Freed J.H.: Rev. Sci. Instrum.59, 1345–1351 (1988)CrossRefADSGoogle Scholar
  6. 6.
    Smith G.M., Lesurf J.C.G., Mitchell R.H., Riedi P.C.: Rev. Sci. Instrum.69, 3924–3937 (1998)CrossRefADSGoogle Scholar
  7. 7.
    Knapp M.J., Krzystek J., Brunel L.C., Hendrickson D.N.: Inorg. Chem.39, 281–288 (2000)CrossRefGoogle Scholar
  8. 8.
    Carver G., Tregenna-Piggott P.L.W., Barra A.L., Neels A., Stride J.A.: Inorg. Chem.42, 5771–5777 (2003)CrossRefGoogle Scholar
  9. 9.
    Ozarowski A., Zvyagin S.A., Reiff W.M., Telser J., Brunel L.C., Krzystek J.: J. Am. Chem. Soc.126, 6574–6575 (2004)CrossRefGoogle Scholar
  10. 10.
    Telser J., van Slageren J., Vongtragool S., Dressel M., Reiff W.M., Zvyagin S.A., Ozarowski A., Krzystek J.: Magn. Res. Chem.43, S130-S139 (2005)CrossRefGoogle Scholar
  11. 11.
    Beinert H., Holm R.H., Münck E.: Science277, 653–659 (1997)CrossRefGoogle Scholar
  12. 12.
    Beinert H.: J. Biol. Inorg. Chem.5, 2–15 (2000)CrossRefGoogle Scholar
  13. 13.
    Holm R.H., Kennepohl P., Solomon E.I.: Chem. Rev.96, 2239–2314 (1996)CrossRefGoogle Scholar
  14. 14.
    Meyer J., Moulis J.M., in: Handbook of Metalloproteins (Messerschmidt A., Huber R., Poulos T., Wieghardt K., eds.), pp. 505–517. Chichester: Wiley 2001.Google Scholar
  15. 15.
    Dauter Z., Wilson K.S., Sieker L., Moulis J.M., Meyer J.: Proc. Natl. Acad. Sci. USA93, 8836–8840 (1996)CrossRefADSGoogle Scholar
  16. 16.
    Schulz C.E., Debrunner P.G.: J. Phys. (Paris) Colloq.37 Suppl. 12, 153–158 (1976)Google Scholar
  17. 17.
    Yoo S.J., Meyer J., Achim C., Petersen J., Hendrich M.P., Münck E.: J. Biol. Inorg. Chem.5, 475–487 (2000)CrossRefGoogle Scholar
  18. 18.
    Wegner P., Beyer M., Schünemann V., Trautwein A.X., Schmidt C., Bönisch H., Gnida M., Meyer-Klaucke W.: Hyperfine Interact.156–157, 293–298 (2004)CrossRefGoogle Scholar
  19. 19.
    Binsted N., Strange R.W., Hasnain S.S.: Biochemistry31, 12117–12125 (1992)CrossRefGoogle Scholar
  20. 20.
    Czernuszewicz R.S., LeGall J., Moura I., Spiro T.G.: Inorg. Chem.25, 696–700 (1986)CrossRefGoogle Scholar
  21. 21.
    Czernuszewicz R.S., Kilpatrick L.K., Koch S.A., Spiro T.G.: J. Am. Chem. Soc.116, 7134–7141 (1994)CrossRefGoogle Scholar
  22. 22.
    Spiro T.G., Czernuszewicz R.S., Han S., in: Biological Applications of Raman Spectroscopy (Spiro T.G., ed.) pp. 523–554. New York: Wiley 1988.Google Scholar
  23. 23.
    Champion P.M., Sievers A.J.: J. Chem. Phys.66, 1819–1825 (1977)CrossRefADSGoogle Scholar
  24. 24.
    Petrouleas V., Simopoulos A., Kostikas A., Coucouvanis D.: J. Phys. (Paris) Colloq.37 Suppl. 12, 159–164 (1976)Google Scholar
  25. 25.
    Godfroy A., Raven N., Sharp R.J.: FEMS Microbiol. Lett.186, 127–132 (2000)CrossRefGoogle Scholar
  26. 26.
    Vielle C., Zeikus G.J.: Microbiol. Mol. Rev.65, 1–43 (2001)CrossRefGoogle Scholar
  27. 27.
    Bönisch H., Schmidt C.L., Bianco P., Ladenstein R.: Acta Crystallogr. D Biol. Crystallogr.61, 990–1004 (2005)CrossRefGoogle Scholar
  28. 28.
    Weihe H., Sim version 2003, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.Google Scholar
  29. 29.
    Eaton W.A., Lovenberg W.: J. Am. Chem. Soc.92, 7195–7198 (1970)CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • A. L. Barra
    • 1
  • A. K. Hassan
    • 2
  • A. Janoschka
    • 3
  • C. L. Schmidt
    • 4
  • V. Schünemann
    • 3
  1. 1.Grenoble High Magnetic Field LaboratoryCentre National de la Recherche ScientifiqueGrenoble Cedex 9France
  2. 2.Bruker Biospin AGFällandenSwitzerland
  3. 3.Department of PhysicsTechnical University of KaiserslauternKaiserslauternGermany
  4. 4.Institute of Biochemistry, Center for Structural and Cell Biology in MedicineUniversity of LübeckLübeckGermany

Personalised recommendations