Stabilization of the collagen structure by hydroxyproline residues

  • M. Bansal
  • C. Ramakrishnan
  • G. N. Ramachandran


The molecular structure of collagen is now accepted to be based on a triple-stranded coiled-coil, in which the three strands are held together predominantly by hydrogen bonds. Recent experimental evidence has shown that the presence of hydroxyproline residues in the third position of the repeating tripeptide unit lends additional stability to the collagen structure. In this paper, we report a model structure, which is supported by these observations. In a model structure proposed earlier, there are two hydrogen bonds per tripeptide unit, one of which is a direct interchain hydrogen bond, while the second hydrogen bond can be formedvia a water molecule. It has now been shown that the same water molecule can also form a hydrogen bond with the oxygen of theγ-hydroxyl group of hydroxyproline in the third position in the sequence (Gly-R2-R3).

This hydroxyl group can also take part in an inter-triple-helix hydrogen bond. Our studies thus show the role played by hydroxyproline residues in the structure and stability of collagen.


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  1. 1.
    Rich, A. and Crick, F. H. C.,J. Mol. Biol. 3 483 (1961).CrossRefGoogle Scholar
  2. 2.
    Ramachandran, G. N., In:Treatise on Collagen, Vol. 1, ed. G. N. Ramachandran, Academic Press, New York, p. 103 (1967).Google Scholar
  3. 3.
    Ramachandran, G. N. and Chandrasekaran, R.,Biopolymers 6 1649 (1968).CrossRefGoogle Scholar
  4. 4.
    Harrington, W. F., Josephs, R. and Segal, D. M.,Ann. Rev. Biochem. 35 599 (1966).CrossRefGoogle Scholar
  5. 5.
    Yee, R. Y., Englander, S. W. and von Hippel, P. H.,J. Mol. Biol. 83 1 (1974).CrossRefGoogle Scholar
  6. 6.
    Ramachandran, G. N., Bansal, M. and Bhatnagar, R. S.,Biochim. Biophys. Acta 322 166 (1973).Google Scholar
  7. 7.
    Ramachandran, G. N., Bansal, M. and Ramakrishnan, C.,Curr. Sci. 44 1 (1975).Google Scholar
  8. 8.
    Jimenez, S., Harsch, M. and Rosenbloom,J. Biochem. Biophys. Res. Commun. 52 106 (1973).CrossRefGoogle Scholar
  9. 9.
    Berg, R. A. and Prockop, D. J.,Biochem. Biophys. Res. Commun. 52 115 (1973).CrossRefGoogle Scholar
  10. 10.
    Ward, A. R. and Mason, P.,J. Mol. Biol. 79 431 (1973).CrossRefGoogle Scholar
  11. 11.
    Ramachandran, G. N. and Kartha, G.,Nature 176 593 (1955).CrossRefGoogle Scholar
  12. 12.
    Ramachandran, G. N. and Sasisekharan, V.,Adv. Protein Chem. 23 283 (1968).CrossRefGoogle Scholar
  13. 13.
    Esipova, N. G. and Chirgadze, Yu. N., InWater in Biological System, ed. L. P. Kayushia, Translation Consulatants Bureau, New York, p. 42 (1969).Google Scholar
  14. 14.
    Fraser, R. D. B. and MacRae, T. P.,Nature 183 179 (1959).CrossRefGoogle Scholar
  15. 15.
    Haly, A. R. and Snaith, J. W.,Biopolymers 10 1681 (1971).CrossRefGoogle Scholar
  16. 16.
    Piez, K. A.,Ann. Rev. Biochem. 37 547 (1968).CrossRefGoogle Scholar
  17. 17.
    Cooper, A.,J. Mol. Biol. 55 123 (1971).CrossRefGoogle Scholar
  18. 18.
    Finch, A. and Ledward, D. A.,Biochim. Biophys. Acta 278 433 (1972).Google Scholar
  19. 19.
    Hulmes, D. J. S., Miller, A., Parry, D. A. D., Piez, K. A. and Woodhead-Galloway, J.,J. Mol. Biol. 9 137 (1973).CrossRefGoogle Scholar
  20. 20.
    Rougvie, M. A. and Bear, R. S.,J. Am. Leather Chemists Assoc. 48 735 (1953).Google Scholar
  21. 21.
    Koetzle, T. F., Lehmann, M. S. and Hamilton, W. C.,Acta Cryst. 29B 231 (1973).Google Scholar
  22. 22.
    Millinova, M. I.,Biofizika 18 416 (1973).Google Scholar
  23. 23.
    Katz, E. P. and Li, ShuTung,J. Mol. Biol. 3 351 (1973).CrossRefGoogle Scholar
  24. 24.
    Piez, K. A., In:Biochemistry of Collagen, G. N. Ramachandran, ed., Plenum Press (In Press).Google Scholar
  25. 25.
    Goldstein, A. and Adams, E.,J. Biol. Chem. 243 55 (1968).Google Scholar
  26. 26.
    Goldstein, A. and Adams, E.,J. Biol. Chem. 245 5478 (1970).Google Scholar
  27. 27.
    Gustavson, K. H.,Nature 175 70 (1955).CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 1975

Authors and Affiliations

  • M. Bansal
    • 1
  • C. Ramakrishnan
    • 1
  • G. N. Ramachandran
    • 1
    • 2
  1. 1.Molecular Biophysics UnitIndian Institute of ScienceBangalore
  2. 2.Department of BiophysicsUniversity of ChicagoChicagoUSA

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