Prevalence of borreliae in ixodid ticks from a floodplain forest ecosystem
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Host-seeking ixodid ticks were sampled in a floodplain forest ecosystem along the lower reaches of the Thaya (Dyje) river in South Moravia (Czech Republic) and Lower Austria during the period 1989–2002. The ticks were examined by dark-field microscopy for borreliae (Borrelia burgdorferi sensu lato, the agent of Lyme borreliosis), and attempts were made to culture the spirochetes in BSK-H medium from preparations containing their high numbers. Isolated borreliae were identified by PCR-RFLP analysis using probes directed against ribosomal spacer genes. A total of 797 nymphal and 719 adult (391 female, 328 male)Ixodes ricinus were examined: 16.2% of nymphs, 28.6% of females and 29.0% of males were positive.Dermacentor reticulatus (70 females, 30 males) andHaemaphysalis concinna (12 nymphs, 8 females, 2 males) were negative for spirochetes. The overall prevalence rate of borreliae inI. ricinus from the floodplain forest is slightly higher than the mean European data (i.e., 14% for nymphs, 21% for adults). The difference in infection rate between nymphal and adult ticks was significant, including the proportion of heavily infected (with >100 borreliae) nymphs (2.1%) vs. adults (7.6%). Prevalence of borreliae inI. ricinus showed a significant decrease during autumn in this ecosystem. Three strains of spirochetes, all of theBorrelia afzelii genomic group, were isolated from femaleI. ricinus. Moreover,Trypanosoma/Crithidia sp. protozoa andDipetalonema rugosicauda nematodes were detected in 0.4% and 1.0%, respectively, of allI. ricinus.
Key wordsLyme borreliosis Borrelia burgdorferi sensu lato Borrelia afzelii Ixodes ricinus floodplain forest Dipetalonema rugosicauda Trypanosoma sp.
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