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Mycotoxin Research

, Volume 17, Supplement 1, pp 24–27 | Cite as

The use of yeast for microbial degradation of some selected mycotoxins

  • Štyriak I 
  • Conková E 
  • Kmec V 
  • Böhm J 
  • Razzazi E 
Article

Abstract

Several biodegradation experiments were carried out using 10 different yeast strains.Saccharomyces spp., Kluyveromyces spp. andRhodotorula spp. were tested for biodegradation of selected mycotoxins (ochratoxin A, nivalenol, deoxynivalenol and fumonisin B1) standardsin vitro. There was confirmed that some yeast strains are able to degrade some mycotoxins. However, great differences between individual strains were observed. Moreover, 12Saccharomyces cerevisiae strains were tested for their potential capability to degrade zearalenone and fumonisins in Sabouraud broth. Two strains were capable to degrade zearalenone totally, one strain decreased the mycotoxin concentration up to 25%, and one strain up to 75% of original amount. Two strains were capable to degrade fumonisins partially.

Keywords

Mycotoxins biodegradation Saccharomyces cerevisiae yeast 

Zusammenfassung

Verschiedene Stämme von Hefen wurdenin vitro zur Biodegradationsstudie herangezogen. ZehnSaccharomyces spp., Kluyveromyces spp. undRhodotorula spp. Stämme wurden auf ihre Biodegradationsfähigkeit ausgewählten Mykotoxinstandards (Ochratoxin A (OA), Nivalenol, Deoxynivalenol und Fumonisin)in vitro getestet. Es konnte gezeigt werden, daß manche Hefestämme imstande sind, einzelne Mykotoxine abzubauen, wobei große Unterschiede zwischen einzelnen Stämmen festgestellt wurden. Zusätzlich wurden 12Saccharomyces cerevisiae Stämme auf ihr potentielles Abbauvermögen von Zearalenon und Fumonisin B1 in einer Nährlösung geprüft. Zwei Stämme waren fähig Zearalenon gänzlich abzubauen, ein Stamm verringerte die anfangskonzentration um 25% und ein weiterer um 75%. Zwei Stämme konnten Fumonisin teilweise abbauen.

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References

  1. 1.
    Beeton S, Bull AT (1989) Biotransformation and detoxification of T-2 toxin by soil and freshwater bacteria. Appl. Environ. Microbiol. 55: 190–197PubMedPubMedCentralGoogle Scholar
  2. 2.
    Böhm J, Grajewski J, Asperger H, Cecon B, Rabus B, Razzazi E (2000) Study on biodegradation of some A- and B-Trichothecenes and ochratoxin A by use of probiotic microorganisms. Mycotoxin Research Vol. 16A 2/70–74Google Scholar
  3. 3.
    He P, Young LG, Forsberg C (1992) Microbial transformation of deoxynivalenol (vomitoxin). Appl. Environ. Microbiol. 58: 3857–3863PubMedPubMedCentralGoogle Scholar
  4. 4.
    Jesenská Z, Šajbidorová I (1991) T-2 toxin degradation by micromycetes. J. Hyg. Epidemiol. Microbiol. Immunol. 35: 41–49PubMedGoogle Scholar
  5. 5.
    Kiessling KH, Pettersson H, Sandholm K, Olsen M (1984) Metabolism of aflatoxin, ochratoxin, zearalenone, and three trichothecenes by intact rumen fluid, rumen protozoa, and rumen bacteria. Appl. Environ. Microbiol. 47: 1070–1073PubMedPubMedCentralGoogle Scholar
  6. 6.
    Megharaj M, Garthwaite I, Thiele JH (1997) Total biodegradation of the oestrogenic mycotoxin zearalenone by a bacterial culture. Lett. Appl. Microbiol. 24: 329–333CrossRefPubMedGoogle Scholar
  7. 7.
    Swanson SP, Nicoletti J, Rood HD Jr, Buck WB, Cote LM (1987) Metabolism of three trichothecene mycotoxins, T-2 toxin, diacetoxyscirpenol and deoxynivalenol, by bovine rumen microorganisms. J. Chromatogr. 414: 335–342CrossRefPubMedGoogle Scholar
  8. 8.
    Swanson SP, Helaszek C, Buck WB, Rood HD Jr, Haschek WM (1988) The role of intestinal microflora in the metabolism of trichothecene mycotoxins. Food Chem. Toxicol. 26: 823–829CrossRefPubMedGoogle Scholar
  9. 9.
    Westlake K, Mackie RI, Dutton MF (1987) T-2 toxin metabolism by ruminal bacteria and its effect on their growth. Appl. Environ. Microbiol. 53: 587–592PubMedPubMedCentralGoogle Scholar
  10. 10.
    Razzazi-Fazeli E, Böhm J, Luf W (1999) Determination of Nivalenol and Deoxynivalenol in wheat using Liquid Chromatography/Mass Spectrometry with Negative Ion Atmospheric Pressure Chemical Ionisation (APCI-) J. Chromatogr A 854, 45–55CrossRefPubMedGoogle Scholar

Copyright information

© Society of Mycotoxin Research and Springer 2001

Authors and Affiliations

  • Štyriak I 
    • 1
  • Conková E 
    • 2
  • Kmec V 
    • 1
  • Böhm J 
    • 3
  • Razzazi E 
    • 3
  1. 1.Institut für Tierphysiologie der NutztiereSlowakische Akademie der WissenschaftenKosiceSlowakei
  2. 2.Institut für PharmakologieVeterinärmedizinische UniversitätKošiceSlowakei
  3. 3.Institut für ErnährungVeterinärmedizinische Universität WienWien

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