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Mycotoxin Research

, Volume 17, Supplement 2, pp 142–145 | Cite as

Monitoring of residues of ochratoxin A in blood and kidney of chicken using HPLC-FLD

  • Fuchs E 
  • Binder EM 
  • Schatzmayr G 
  • Heidler D 
  • Klimitsch A 
  • Krska R 
Article
  • 43 Downloads

Abstract

Detoxification of ochratoxin A can be achieved by chemical or enzymatic hydrolyzation, the products of such reactions are ochratoxin α and phenylalanine. Ochratoxin α like ochratoxin A, is a fluorescing molecule, therefore sensitive analysis is possible at very low concentration levels. Methods have been established that make it possible to look for residues of ochratoxin A and its main metabolite ochratoxin α in blood and tissues at very low concentration levels. Plasma is extracted by the use of small amounts of chloroform; the extract is cleaned with water and afterwards evaporated to dryness]. The residue is re-dissolved and analysed by HPLC-FLD. Using this method a limit of detection of 0.5μg/l for both ochratoxin A and ochratoxin α can be reached.

Keywords

ochratoxin A ochratoxin alpha blood kidney 

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Copyright information

© Society of Mycotoxin Research and Springer 2001

Authors and Affiliations

  • Fuchs E 
    • 1
  • Binder EM 
    • 2
  • Schatzmayr G 
    • 2
  • Heidler D 
    • 2
  • Klimitsch A 
    • 2
  • Krska R 
    • 1
  1. 1.IFA-TullnCenter for Analytical ChemistryTulln
  2. 2.Biomin® GTI GmbHHerzogenburg

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