Andrologie

, Volume 15, Issue 1, pp 41–47

La nutraceutiques (alicaments) et compléments alimentaires dans le traitement de l’infertilité masculine

Nutrition, Environnement en Andrologie Thèrapeutique

Resume

Plusieurs essais contrôlés et correctement validés apportent des preuves que des compléments alimentaires contenant des substances particulières peuvent améliorer la qualité et la fonction spermatiques d’hommes hypofertiles. Parmi ces compléments figurent les antioxydants astaxanthine, tocophérols et ubiquinone Q10, des acides gras essentiels du groupe oméga 3, le zinc et l’acide folique. Il y a des études qui suggèrent que certains extraits de plantes et des lignans ont également des effets bénéfiques. L’administration d’un nutraceutique (Qualisperm ®, Nutriphyt, Oostkamp, Belgique) contenant ces substances augmente la probabilité d’une conception spontanée et d’une grossesse menée à terme. Bien que les mécanismes exacts de l’action de ces compléments alimentaires sur la spermatogenèse et la fonction spermatique n’aient pas encore été entièrement découverts, il semble concevable que ces compléments agissent directement sur les cellules de Sertoli et améliore la fonction épididymaire. En tant que compléments alimentaires exempts de toute toxicité, les nutraceutiques devraient également être pris en considération avant de procéder à une fertilisation in vitro et à une ICSI, afin de réduire les dégâts oxydatifs à l’ADN des spermatozoïdes.

Mots clés

nutraceutiques alicaments infertilité antioxydants ADN oxydé acides gras 

Nutraceuticals and food supplementation for the treatment of the subfertile male

Abstract

Several controlled and well-validated trials provide evidence that food supplementation with particular substances can improve semen quality and function of subfertile men. These include the antioxidants astaxanthin, tocopherols and ubiquinone Q10, essential fatty acids of the omega-3 group, zinc, and folic acid. There is suggestive evidence that certain plant extracts and lignans may equally exert beneficial effects. Supplementation with a nutraceutical (Qualisperm®, Nutriphyt, Oostkamp, Belgium) containing these substances increases the probability of spontaneous conception and successful pregnancy. Although the exact mechanisms of action of these supplements on spermatogenesis and sperm function remain to be unravelled, a direct effect on the cells of Sertoli and via epididymal function seems conceivable. Nutraceutical food supplementation should also be considered beforein vitro fertilization and ICSI, in order to reduce the oxidative damage to sperm DNA.

Key words

nutraceuticals infertility fatty acids antioxidants astaxanthin 

References

  1. 1.
    ADLERCREUTZ H., BANNWART C., WAHALA K. et al.: Inhibition of human aromatase by mammalian lignans and isoflavonoid phytoestrogens. J. Steroid Biochem. Mol. Biol., 1993, 44: 147–153.PubMedCrossRefGoogle Scholar
  2. 2.
    AYDIN S., INCI O., ALAGOL B.: The role of arginine, indomethacin and kallikrein in the treatment of oligoasthenospermia. Int. Urol. Nephrol., 1995, 27: 199–202.PubMedCrossRefGoogle Scholar
  3. 3.
    BARTELLINI M., CANALE D., IZZO P.L., GIORGI P.M., MESCHINI P., MECHINI-FABRIS G.F.: L-carnitine and acetylcarnitine in human sperm with normal and reduced motility. Acta Eur. Fertil., 1987, 18: 29–31.PubMedGoogle Scholar
  4. 4.
    BAUMANN J., VON BRUCHHAUSEN F., WURM G.: Flavonoids and related compounds as inhibition of arachidonic acid peroxidation. Prostaglandins, 1980, 20: 627–639.PubMedCrossRefGoogle Scholar
  5. 5.
    BERNARD D., CHRISTOPHE A., DELANGHE J., LANGLOIS M., DE BUYZERE M., COMHAIRE F.: The effect of supplementation with an antioxidant preparation on LDL-oxidation is determined by haptoglobin polymorphism. Redox. Rep., 2003, 8: 41–46.PubMedCrossRefGoogle Scholar
  6. 6.
    BONDE J.P., JOFFE M., APOSTOLI P. et al.: Sperm count and chromatin structure in men exposed to inorganic lead: lowest adverse effect levels. Occup. Environ. Med., 2002, 59: 234–242.PubMedCrossRefGoogle Scholar
  7. 7.
    CHO K.J., YUN C.H., PACKER L., CHUNG A.S.: Inhibition mechanisms of bioflavonoids extracted from the bark of Pinus maritima on the expression of proinflammatory cytokines. Ann. N. Y. Acad. Sci., 2001, 928: 141–156.PubMedCrossRefGoogle Scholar
  8. 8.
    CHRISTOPHE A., ZALATA A., MAHMOUD A., COMHAIRE F. Fatty acid composition of sperm phospholipids and its nutritional implications. Middle East Fert. Soc. J., 1998, 3: 46–53.Google Scholar
  9. 9.
    CINTI D.L., COOK L., NAGI M.N., SUNEJA S.K.: The fatty acid chain elongation system of mammalian endoplasmic reticulum. Prog. Lipid Res., 1992, 31: 1–51.PubMedCrossRefGoogle Scholar
  10. 10.
    COMHAIRE F.: Clinical andrology: from evidence-base to ethics. The ‘E’ quintet in clinical andrology. Hum. Reprod., 2000, 15: 2067–2071.PubMedCrossRefGoogle Scholar
  11. 11.
    COMHAIRE F., VERMEULEN L.: Effect of high dose oral Kallikrein treatment in men with idiopathic subfertility: evaluation by means of in vitro penetration test of zona free hamster ova. Int. J. Androl., 1983, 6: 168–172.PubMedCrossRefGoogle Scholar
  12. 12.
    COMHAIRE F.H., CHRISTOPHE A.B., ZALATA A.A., DHOOGE W.S., MAHMOUD A.M., DEPUYDT C.E.: The effects of combined conventional treatment, oral antioxidants and essential fatty acids on sperm biology in subfertile men. Prostaglandins Leukot. Essent. Fatty Acids, 2000, 63: 159–165.CrossRefGoogle Scholar
  13. 13.
    COMHAIRE F.H., GAREM Y.F.E., MAHMOUD A., EERTMANS F., SCHOONJANS F.: Combined conventional/antioxidant “Astaxanthin” treatment for male infertility: a double blind randomized trial. Asian J. Androl., 2004, sous presse.Google Scholar
  14. 14.
    COMHAIRE F.H., MAHMOUD A.M.: Editorial commentary. J. Androl., 2004, 25: 771–772.Google Scholar
  15. 15.
    COSTA M., CANALE D., FILICORI M., D’LDDIO S., LENZI A.: L-carnitine in idiopathic asthenozoospermia: a multicenter study. Italian Study Group on Carnitine and Male Infertility. Andrologia, 1994, 26: 155–159.PubMedCrossRefGoogle Scholar
  16. 16.
    DAWSON E.B., HARRIS W.A., TETER M.C., POWELL L.C.: Effect of ascorbic acid supplementation on the sperm quality of smokers. Fertil. Steril., 1992, 58: 1034–1039.PubMedGoogle Scholar
  17. 17.
    DE ALOYSIO D., MANTUANO R., MAULONI M., NICOLETTI G.: The clinical use of arginine aspartate in male infertility. Acta Eur. Fertil., 1982, 13: 133–167.PubMedGoogle Scholar
  18. 18.
    DEBAUN M.R., NIEMITZ E.L., FEINBERG A.P.: Association of in vitro fertilization with Beckwith-Wiedemann syndrome and epigenetic alterations of LIT1 and H19. Am. J. Hum. Genet., 2003, 72: 156–160.PubMedCrossRefGoogle Scholar
  19. 19.
    DEPUYDT C.E., MAHMOUD A.M., DHOOGE W.S., SCHOONJANS F.A., COMHAIRE F.H.: Hormonal regulation of inhibin B secretion by immature rat sertoli cells in vitro: possible use as a bioassay for estrogen detection. J. Androl., 1999, 20: 54–62.PubMedGoogle Scholar
  20. 20.
    EDWARDS R.G., LUDWIG M.: Are major defects in children conceived in vitro due to innate problems in patients or to induced genetic damage? Reprod. Biomed. Online, 2003, 7: 131–138.PubMedCrossRefGoogle Scholar
  21. 21.
    FRAGA C.G., MOTCHNIK P.A., SHIGENAGA M.K., HELBOCK H.J., JACOB R.A., AMES B.N.: Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proc. Natl. Acad. Sci. U. S. A., 1991, 88: 11003–11006.PubMedCrossRefGoogle Scholar
  22. 22.
    GOLAN R., WEISSENBERG R., LEWIN L.M.: Carnitine and acetylcarnitine in motile and immotile human spermatozoa. Int. J. Androl., 1984, 7: 484–494.PubMedCrossRefGoogle Scholar
  23. 23.
    GONZALES G.F., CORDOVA A., GONZALES C., CHUNG A., VEGA K., VILLENA A.: Lepidium meyenii (Maca) improved semen parameters in adult men. Asian J. Androl., 2001, 3: 301–303.PubMedGoogle Scholar
  24. 24.
    GONZALES G.F., RUIZ A., GONZALES C., VILLEGAS L., CORDOVA A.: Effect of Lepidium meyenii (maca) roots on spermatogenesis of male rats. Asian J. Androl., 2001, 3: 231–233.PubMedGoogle Scholar
  25. 25.
    GOTO S., KOGURE K., ABE K. et al.: Efficient radical trapping at the surface and inside the phospholipid membrane is responsible for highly potent antiperoxidative activity of the carotenoid astaxanthin. Biochim. Biophys. Acta, 2001, 1512: 251–258.PubMedCrossRefGoogle Scholar
  26. 26.
    GREEN N.S.: Risks of birth defects and other adverse outcomes associated with assisted reproductive technology. Pediatrics, 2004, 114: 256–259.PubMedCrossRefGoogle Scholar
  27. 27.
    HANSEN M., KURINCZUK J.J., BOWER C., WEBB S.: The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. N. Engl. J. Med., 2002, 346: 725–730.PubMedCrossRefGoogle Scholar
  28. 28.
    HUGHES C.M., LEWIS S.E., MCKELVEY-MARTIN V.J., THOMPSON W.: The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity. Hum. Reprod., 1998, 13: 1240–1247.PubMedCrossRefGoogle Scholar
  29. 29.
    HURTADO DE CATALFO G.E., DE GOMEZ DUMM I.N.: Polyunsaturated fatty acid biosynthesis from [1–14C]20:3 n-6 acid in rat cultured Sertoli cells. Linoleic acid effect. Int. J. Biochem. Cell Biol., 2002, 34: 525–532.PubMedCrossRefGoogle Scholar
  30. 30.
    IRVINE D.S., TWIGG J.P., GORDON E.L., FULTON N., MILNE P.A., AITKEN R.J.: DNA integrity in human spermatozoa: relationships with semen quality. J. Androl., 2000, 21: 33–44.PubMedGoogle Scholar
  31. 31.
    IWAMOTO T., HOSODA K., HIRANO R. et al.: Inhibition of low-density lipoprotein oxidation by astaxanthin. J. Atheroscler. Thromb., 2000, 7: 216–222.PubMedGoogle Scholar
  32. 32.
    JEULIN C., LEWIN L.M. Role of free L-carnitine and acetyl-L-carnitine in post-gonadal maturation of mammalian spermatozoa. Hum. Reprod. Update, 1996, 2: 87–102.PubMedCrossRefGoogle Scholar
  33. 33.
    KATZ P., NACHTIGALL R., SHOWSTACK J.: The economic impact of the assisted reproductive technologies. Nat. Cell Biol., 2002, 4 Suppl: s29-s32.PubMedCrossRefGoogle Scholar
  34. 34.
    KENT-FIRST M.G., KOL S., MUALLEM A. et al.: The incidence and possible relevance of Y-linked microdeletions in babies born after intracytoplasmic sperm injection and their infertile fathers. Mol. Hum. Reprod., 1996, 2: 943–950.PubMedCrossRefGoogle Scholar
  35. 35.
    KESSOPOULOU E., POWERS H.J., SHARMA K.K. et al.: A double-blind randomized placebo cross-over controlled trial using the antioxidant vitamin E to treat reactive oxygen species associated male infertility. Fertil. Steril., 1995, 64: 825–831.PubMedGoogle Scholar
  36. 36.
    KOHENGKUL S., TANPHAICHITR V., MUANGMUN V., TANPHAICHITR N.: Levels of L-carnitine and L-O-acetylcarnitine in normal and infertile human semen: a lower level of L-O-acetycarnitine in infertile semen. Fertil. Steril., 1977, 28: 1333–1336.PubMedGoogle Scholar
  37. 37.
    KOUDSTAAL J., BRUINSE H.W., HELMERHORST F.M., VERMEIDEN J.P., WILLEMSEN W.N., VISSER G.H. Obstetric outcome of twin pregnancies after in vitro fertilization: a matched control study in four Dutch University hospitals. Hum. Reprod., 2000, 15: 935–940.PubMedCrossRefGoogle Scholar
  38. 38.
    LAMBERT R.D.: Safety issues in assisted reproduction technology: the children of assisted reproduction confront the responsible conduct of assisted reproductive technologies. Hum. Reprod., 2002, 17: 3011–3015.PubMedCrossRefGoogle Scholar
  39. 39.
    LENZI A., CULASSO F., GANDINI L., LOMBARDO F., DONDERO F.: Placebo-controlled, double-blind, cross-over trial of glutathione therapy in male infertility. Hum. Reprod., 1993, 8: 1657–1662.PubMedGoogle Scholar
  40. 40.
    LENZI A., LOMBARDO F., SGRO P. et al.: Use of carnitine therapy in selected cases of male factor infertility: a doubleblind crossover trial. Fertil. Steril., 2003, 79: 292–300.PubMedCrossRefGoogle Scholar
  41. 41.
    LEWIN A., LAVON H.: The effect of coenzyme Q10 on sperm motility and function. Mol. Aspects Med., 1997, 18 Suppl: S213–9.CrossRefGoogle Scholar
  42. 42.
    MA K., SHARKEY A., KIRSCH S. et al.: Towards the molecular localisation of the AZF locus: mapping of microdeletions in azoospermic men within 14 subintervals of interval 6 of the human Y chromosome. Hum. Mol. Genet., 1992, 1: 29–33.PubMedCrossRefGoogle Scholar
  43. 43.
    MAHER E.R., AFNAN M., BARRATT C.L.: Epigenetic risks related to assisted reproductive technologies: epigenetics, imprinting, ART and icebergs? Hum. Reprod., 2003, 18: 2508–2511.PubMedCrossRefGoogle Scholar
  44. 44.
    MAHMOUD A., KISS P., KAUFMAN J.M., COMHAIRE F., ASCLEPIOS: The influence of age and lead exposure on inhibin B serum levels in men. Int. J. Androl., 2000, 23 (suppl. 1): PO94.Google Scholar
  45. 45.
    MAHMOUD A.M., COMHAIRE F.H., DEPUYDT C.E.: The clinical and biological significance of serum inhibins in subfertile men. Reprod. Toxicol., 1998, 12: 591–599.PubMedCrossRefGoogle Scholar
  46. 46.
    MAYERHOFER A., MEINEKE V., KÖHN F.M., FRUNGIERI M.: Cyclooxygenase (COX-2) in male infertility: a new link between prostaglandins and testicular fibrosis? Andrologia, 2002, 34: 272–273.Google Scholar
  47. 47.
    MEACHEM S.J., NIESCHLAG E., SIMONI M.: Inhibin B in male reproduction: pathophysiology and clinical relevance. Eur. J. Endocrinol., 2001, 145: 561–571.PubMedCrossRefGoogle Scholar
  48. 48.
    MENCHINI-FABRIS G.F., CANALE D., IZZO P.L., OLIVIERI L., BARTELLONI M.: Free L-carnitine in human semen: its variability in different andrologic pathologies. Fertil. Steril., 1984, 42: 263–267.PubMedGoogle Scholar
  49. 49.
    MOLL A.C., IMHOF S.M., CRUYSBERG J.R., SCHOUTEN-VAN MEETEREN A.Y., BOERS M., VAN LEEUWEN F.E.: Incidence of retinoblastoma in children born after in vitro fertilisation. Lancet, 2003, 361: 309–310.PubMedCrossRefGoogle Scholar
  50. 50.
    MONCADA M.L., VICARI E., CIMINO C., CALOGERO A.E., MONGIOI A., D’AGATA R.: Effect of acetylcarnitine treatment in oligoasthenospermic patients. Acta Eur. Fertil., 1992, 23: 221–224.PubMedGoogle Scholar
  51. 51.
    PALERMO G., JORIS H., DEVROEY P., VAN STEIRTEGHEM A.C.: Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet, 1992, 340: 17–18.PubMedCrossRefGoogle Scholar
  52. 52.
    PINBORG A., LOFT A., SCHMIDT L., GREISEN G., RASMUSSEN S., ANDERSEN A.N.: Neurological sequelae in twins born after assisted conception: controlled national cohort study. Br. Med. J., 2004, 329: 311.CrossRefGoogle Scholar
  53. 53.
    PRYOR J.P., BLANDY J.P., EVANS P., CHAPUT D.S.D., USHERWOOD M.: Controlled clinical trial of arginine for infertile men with oligozoospermia. Br. J. Urol., 1978, 50: 47–50.PubMedCrossRefGoogle Scholar
  54. 54.
    ROHDEWALD P.: A review of the French maritime pine bark extract (Pycnogenol), a herbal medication with a diverse clinical pharmacology. Int. J. Clin. Pharmacol. Ther., 2002, 40: 158–168.PubMedGoogle Scholar
  55. 55.
    ROLF C., COOPER T.G., YEUNG C.H., NIESCHLAG E.: Antioxidant treatment of patients with asthenozoospermia or moderate oligoasthenozoospermia with high-dose vitamin C and vitamin E: a randomized, placebo-controlled, doubleblind study. Hum. Reprod., 1999, 14: 1028–1033.PubMedCrossRefGoogle Scholar
  56. 56.
    ROSEFF S.J., GULATI R.: Improvement of sperm quality by Pycnogenol. Eur. Bull. Drug Res., 1999, 7: 33–36.Google Scholar
  57. 57.
    RUIZ-PESINI E., ALVAREZ E., ENRIQUEZ J.A., LOPEZ-PEREZ M.J.: Association between seminal plasma carnitine and sperm mitochondrial enzymatic activites. Int. J. Androl., 2001, 24: 335–340.PubMedCrossRefGoogle Scholar
  58. 58.
    SAETHER T., TRAN T.N., ROOTWELT H., CHRISTOPHERSEN B.O., HAUGEN T.B.: Expression and regulation of delta5-desaturase, delta6-desaturase, stearoyl-coenzyme A (CoA) desaturase 1, and stearoyl-CoA desaturase 2 in rat testis. Biol. Reprod., 2003, 69: 117–124.PubMedCrossRefGoogle Scholar
  59. 59.
    SCHIEVE L.A., RASMUSSEN S.A., BUCK G.M., SCHENDEL D.E., REYNOLDS M.A., WRIGHT V.C.: Are children born after assisted reproductive technology at increased risk for adverse health outcomes? Obstet. Gynecol., 2004, 103: 1154–1163.PubMedGoogle Scholar
  60. 60.
    SCHILL W.B., KRIZIC A., RJOSK H.: Determination of various semen parameters and sex hormone levels in subfertile men during kallikrein therapy. Adv. Exp. Med. Biol., 1979, 120A: 537–546.PubMedGoogle Scholar
  61. 61.
    SCOTT R., MACPHERSON A., YATES R.W., HUSSAIN B., DIXON J.: The effect of oral selenium supplementation on human sperm motility. Br. J. Urol., 1998, 82: 76–80.PubMedGoogle Scholar
  62. 62.
    SHARPE R.M. The ‘oestrogen hypothesis’-where do we stand now? Int. J. Androl., 2003, 26: 2–15.PubMedCrossRefGoogle Scholar
  63. 63.
    SKAKKEBAEK N.E.: Testicular dysgenesis syndrome: new epidemiological evidence. Int. J. Androl., 2004, 27: 189–191.PubMedCrossRefGoogle Scholar
  64. 64.
    SOUFIR J.C., DUCOT B., MARSON J. et al.: Levels of seminal free L(−) carnitine in fertile and infertile men. Int. J. Androl., 1984, 7: 188–197.PubMedCrossRefGoogle Scholar
  65. 65.
    STEPTOE P.C., EDWARDS R.G.: Birth after the reimplantation of a human embryo. Lancet, 1978, 2: 366.PubMedCrossRefGoogle Scholar
  66. 66.
    STROMBERG B., DAHLQUIST G., ERICSON A., FINNSTROM O., KOSTER M., STJERNQVIST K.: Neurological sequelae in children born after in vitro fertilisation: a population-based study. Lancet, 2002, 359: 461–465.PubMedCrossRefGoogle Scholar
  67. 67.
    SUTCLIFFE A.G., TAYLOR B., LI J., THORNTON S., GRUDZINSKAS J.G., LIEBERMAN B.A.: Children born after intracytoplasmic sperm injection: population control study. Br. Med. J., 1999, 318: 704–705.Google Scholar
  68. 68.
    TANPHAICHITR N.: In vitro stimulation of human sperm motility by acetylcarnitine. Int. J. Fertil., 1977, 22: 85–91.PubMedGoogle Scholar
  69. 69.
    TIEPOLO L., ZUFFARDI O.: Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome long arm. Hum. Genet., 1976, 34: 119–124.PubMedCrossRefGoogle Scholar
  70. 70.
    TRAN T.N., RETTERSTOL K., CHRISTOPHERSEN B.O.: Metabolism of long-chain polyunsaturated fatty acids in testicular cells. In: De Vriese S.R., Christophe A.B. eds. Male fertility and lipid metabolism. Illinois, AOCS Press, 2003: 11–22.Google Scholar
  71. 71.
    VAN DER VEN K., PESCHKA B., MONTAG M. et al.: Increased frequency of congenital chromosomal aberrations in female partners of couples undergoing intracytopiasmic sperm injection. Hum. Reprod., 1998, 13: 48–54.PubMedCrossRefGoogle Scholar
  72. 72.
    VAN DISSEL-EMILIANI F.M., GROOTENHUIS A.J., DE JONG F.H., DE ROOLJ D.G.: Inhibin reduces spermatogonial numbers in testes of adult mice and Chinese hamsters. Endocrinology, 1989, 125: 1899–1903.PubMedGoogle Scholar
  73. 73.
    VANHOORNE M., COMHAIRE F., DE BACQUER D.: Epidemiological study of the effects of carbon disulfide on male sexuality and reproduction. Arch. Environ. Health, 1994, 49: 273–278.PubMedCrossRefGoogle Scholar
  74. 74.
    VIRGILI F., KIM D., PACKER L.: Procyanidins extracted from pine bark protect alpha-tocopherol in ECV 304 endothelial cells challenged by activated RAW 264.7 macrophages: role of nitric oxide and peroxynitrite. FEBS Lett., 1998, 431: 315–318.PubMedCrossRefGoogle Scholar
  75. 75.
    VITALI G., PARENTE R., MELOTTI C.: Carnitine supplementation in human idiopathic asthenospermia: clinical results. Drugs Exp. Clin. Res., 1995, 21: 157–159.PubMedGoogle Scholar
  76. 76.
    VOLIANI S., BERTOZZI M.A., ROSSI P., MENCHINI-FABRIS G.F.: The treatment of male infertility with L-carnitine/L-acetyl carnitine. XVII National Congress, Societa Italiana Andrologia SIA, Venezia 2001, 2001, 8: 122.Google Scholar
  77. 77.
    WANG C., MAKELA T., HASE T., ADLERCREUTZ H., KURZER M.S.: Lignans and flavonoids inhibit aromatase enzyme in human preadipocytes. J. Steroid Biochem. Mol. Biol., 1994, 50: 205–212.PubMedCrossRefGoogle Scholar
  78. 78.
    WENNERHOLM U., BERGH C., HAMBERGER L. et al.: Incidence of congenital malformations in children born after ICSI. Hum. Reprod., 2000, 15: 944–948.PubMedCrossRefGoogle Scholar
  79. 79.
    WILDMAN R.E.C., MEDEIROS D.M.: Advanced human nutrition. London, CRC Press, 2000: 370–371.Google Scholar
  80. 80.
    WONG W.Y., MERKUS H.M., THOMAS C.M., MENKVELD R., ZIELHUIS G.A., STEEGERS-THEUNISSEN R.P.: Effects of folic acid and zinc sulfate on male factor subfertility: a doubleblind, randomized, placebo-controlled trial. Fertil. Steril., 2002, 77: 491–498.PubMedCrossRefGoogle Scholar
  81. 81.
    ZALATA A.A., CHRISTOPHE A.B., DEPUYDT C.E., SCHOONJANS F., COMHAIRE F.H.: The fatty acid composition of phospholipids of spermatozoa from infertile patients. Mol. Hum. Reprod., 1998, 4: 111–118.PubMedCrossRefGoogle Scholar
  82. 82.
    ZHENG B.L., HE K., KIM C.H. et al.: Effect of a lipidic extract from lepidium meyenii on sexual behavior in mice and rats. Urology, 2000, 55: 598–602.PubMedCrossRefGoogle Scholar

Copyright information

© Société d’Andrologie de Langue Française 2005

Authors and Affiliations

  1. 1.Centre d’andrologie et d’endocrinologie reproductiveHôpital Universitaire de GandGandBelgique

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