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Neurotoxicity Research

, Volume 6, Issue 4, pp 333–342 | Cite as

Common patterns of Bcl-2 family gene expression in two traumatic brain injury models

  • Kenneth I. StraussEmail author
  • Raj K. Narayan
  • Ramesh Raghupathi
Article

Abstract

Cell death/survival following traumatic brain injury (TBI) may be a result of alterations in the intracellular ratio of death and survival factors. Bcl-2 family genes mediate both cell survival and the initiation of cell death. Using lysate RNase protection assays, mRNA expression of the anti-cell death genes Bcl-2 and Bcl-xL, and the pro-cell death gene Bax, was evaluated following experimental brain injuries in adult male Sprague-Dawley rats. Both the lateral fluid-percussion (LFP) and the lateral controlled cortical impact (LCI) models of TBI showed similar patterns of gene expression. Anticell death bcl-2 and bcl-xL mRNAs were attenuated early and tended to remain depressed for at least 3 days after injury in the cortex and hippocampus ipsilateral to injury. Pro-cell death bax mRNA was elevated in these areas, usually following the decrease in anti-cell death genes. These common patterns of gene expression suggest an important role for Bcl-2 genes in cell death and survival in the injured brain. Understanding the regulation of these genes may facilitate the development of new therapeutic strategies for a condition that currently has no proven pharmacologic treatments.

Keywords

Apoptosis mRNA measurement Cerebral cortex Hippocampus Traumatic brain injury Animal models Gene expression patterns 

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Copyright information

© Springer 2004

Authors and Affiliations

  • Kenneth I. Strauss
    • 1
    Email author
  • Raj K. Narayan
    • 1
  • Ramesh Raghupathi
    • 2
  1. 1.Department of NeurosurgeryUniversity of Cincinnati College of MedicineCincinnatiUSA
  2. 2.Drexel University, MCP/Hahnemann Medical CollegePhiladelphia

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