Journal of Plant Biology

, Volume 49, Issue 4, pp 309–314

Molecular genetic analysis of tandemly located glycosyltransferase genes,UGT73B1,UGT73B2, andUGT73B3, inArabidopsis thaliana

Article

DOI: 10.1007/BF03031161

Cite this article as:
Lim, C.E., Ahn, JH. & Lim, J. J. Plant Biol. (2006) 49: 309. doi:10.1007/BF03031161

Abstract

In theArabidopsis genome, approximately 120 UDP-glycosyltransferases (UGTs) have been annotated. They generally catalyze the transfer of sugars to various acceptor molecules, including flavonoids. To better understand their physiological roles, we analyzed a tandemly located putative flavonoid UGT cluster comprisingUGT73B1, UGT73B2, andUGT73B3 on Chromosome IV. We then isolated four loss-of-function mutations —ugt73b1- 1, ugt73b2- 1, ugt73b3- 1, andugt73b3- 2. In our expression analysis, the closely related UCTs exhibited tissue-specific patterns of expression that were severely altered in their respective mutant plants. For example,UGT73B2 was up-regulated inugt73b1- 1, whereasUGT73B7 was highly expressed inugt73b2- 1, ugt73b3- t, andugt73b3- 2. Interestingly, each recessive mutant was resistant to methyl viologen (paraquat), an herbicide thought to cause oxidative stress. Our results suggest thatUGTs play an important role in the glycosylation pathways when responding to oxidative stress.

Keywords

Arabidopsis flavonoids glycosylation methyl viologen oxidative stress UDP-glycosyltransferases 

Abbreviations

MV

methyl viologen

RT-qPCR

quantitative reverse transcriptase polymerase chain reaction;

UCTs

UDP-glycosyltransferases.

Copyright information

© The Botanical Society of Korea 2006

Authors and Affiliations

  1. 1.Division of Bioscience and Biotechnology, Bio/Molecular Informatics CenterKonkuk UniversitySeoulKorea

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