Differential expression of the potato proteinase inhibitor II promoter in transgenic tobacco
- 76 Downloads
We studied temporal and spatial expression patterns of the potato proteinase inhibitor II (PI-II) promoter, using transgenic tobacco (Nkotiana tabacum L cv. Xanthi) plants that carried a fusion between the PI-II promoter and the chloramphenicol acetyltransferase (cat) gene. Pl-ll promoter activity was low when plants were young, but increased as plants grew. In 8-week-old plants, old leaves showed higher activity than young leaves. At flowering stage (ca. 15 weeks), the overall promoter activity was reduced to a lower level except in the petals. Compared with stems or petioles at the flowering stage, the roots and floral organs showed minimal activity for the Pl-ll promoter. We used several environmental stimuli to examine the induction of the Pl-ll promoter in different organs. Promoter induction was effected by wounding or methyl jasmonate in stems, petioles, sepals, and leaves. The induction was highest in leaves, as was sucrose-enhanced wound induction. These results suggest that the Pl-ll gene is temporally and spatially regulated. We also established a transient assay system in tobacco BY2 suspension cells to elucidate the upstream regulatory region of the Pl-ll promoter. A field strength of 0.75 kV/cm and 400 μF capacitance were optimal electroporation conditions for our transient assay.
Keywordschloramphenicol acetyltransferase electroporation promoter proteinase inhibitor transgenic tobacco
Unable to display preview. Download preview PDF.
- An G, Ebert PR, Mitra A, Ha SB (1988a) Binary vectors,In SB Gelvin, RA Schilperoort, eds, Plant Molecular Biology Manual, Kluwer Academic Publishers, Dordrecht, pp A3: 1–19Google Scholar
- Anderson JM (1989) Membrane-derived fatty acids as precursors to second messengers,In WF Boss, DJ Morre, NS Allen, eds, Second Messengers in Plant Growth and Development, Alan R Liss, New York, pp 181–212Google Scholar
- Bishop PD, Pearce G, Bryant JE, Ryan CA (1984) Isolation and characterization of the proteinase inhibitor-inducing factor from tomato leaves. J Biochem 259: 13172–13177Google Scholar
- Nagy F, Odell JT, Morelli G, Chua N-H (1985) Relevance to agriculture in the Eighties,In M Zaitlin, P Day, A Hol-laender, eds, Biotechnology in Plant Science. Academic Press, New York, pp 227–236Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning, A Laboratory Manual, Ed 2, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar