Analysis of flavonoids and characterization of theOsFNS gene involved in flavone biosynthesis in Rice

  • Jeong Ho Kim
  • Young Min Cheon
  • Bong -Gyu Kim
  • Joong -Hoon Ahn
Article
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Abstract

The major flavonoids in rice leaves were analyzed via LC-MS/MS after their total flavonoid extracts were hydrolyzed. The most abundant flavones were apigenin, luteolin, and tricetin. Of these, tricetin was methylated at its 3′ and 5′-hydroxyl group to form tricin, which was probablyO-glycosylated. Both 3′-O-methylated luteolin and luteolin were found in theC-glycosylated form while apigenin wasC-glycosylated. We also cloned and characterizedOsFNS, which catalyzes the reaction from flavanone (naringenin) to flavone (apigenin). Analysis of the reaction product with recombinant OsFNS showed that it indeed converts naringenin to apigenin.

Key words

flavones flavone synthase flavonoid analysis Oryza sativa 
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Copyright information

© The Botanical Society of Korea 2008

Authors and Affiliations

  • Jeong Ho Kim
    • 1
  • Young Min Cheon
    • 1
  • Bong -Gyu Kim
    • 1
  • Joong -Hoon Ahn
    • 1
  1. 1.Department of Bioscience and BiotechnologyBio/Molecular Informatics Center, Konkuk UniversitySeoulKorea

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