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Journal of Plant Biology

, Volume 50, Issue 5, pp 600–604 | Cite as

The serine proteinase inhibitor OsSerpin is a potent tillering regulator in rice

  • Song Yion Yeu
  • Bong Soo Park
  • Wan Gyu Sang
  • Yang Do Choi
  • Min Chul Kim
  • Jong Tae Song
  • Nam-Chon Paek
  • Hee-Jong Koh
  • Hak Soo Seo
Article

Abstract

Tillering in rice (Oryza sativa L.) is an important agronomic trait that enhances grain production. A tiller is a specialized grain-bearing branch that is formed on a non-elongated basal internode that grows independently of the mother stem. Transgenic rice over-expressing the transcription factorOsTB1, a homologue of maizeTB1 (Teosinte Branched 1), exhibits markedly reduced lateral branching without the propagation of axillary buds being affected. However, the tillering mechanism remains unknown. Therefore, to further understand that mechanism, we applied proteomics methodology to isolate the proteins involved. Using two-dimensional gel electrophoresis and mass spectrometry, our analysis of the basal nodes from two rice cultivars that differ in their numbers of tillers showed that a rice serine proteinase inhibitor, OsSerpin, accumulates in great amounts in high-tillering ‘Hwachung’ rice. Northern blot analysis revealed that much moreOsSerpin transcript is found in ‘Hwachung’ than in relatively low-tillering ‘Hanmaeum’, likely because of high levels of transcription. Therefore, our data suggest that OsSerpin content determines the extent of lateral branching.

Keywords

basal node OsSerpin Proteomics Rice tillering 

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Copyright information

© The Botanical Society of Korea 2007

Authors and Affiliations

  • Song Yion Yeu
    • 2
    • 3
  • Bong Soo Park
    • 1
    • 3
  • Wan Gyu Sang
    • 1
    • 3
  • Yang Do Choi
    • 2
    • 3
  • Min Chul Kim
    • 4
  • Jong Tae Song
    • 5
  • Nam-Chon Paek
    • 1
    • 3
  • Hee-Jong Koh
    • 1
    • 3
  • Hak Soo Seo
    • 1
    • 3
    • 6
  1. 1.Department of Plant ScienceSeoul National UniversitySeoulKorea
  2. 2.School of Agricultural BiotechnologySeoul National UniversitySeoulKorea
  3. 3.Research Institute for Agriculture and Life SciencesSeoul National UniversitySeoulKorea
  4. 4.Division of Agriculture and Life SciencesGycongsang National UniversityJinjuKorea
  5. 5.School of Applied BioscienccsKyungpook National UniversityDaeguKorea
  6. 6.Bio-MAX InstituteSeoul National UniversitySeoulKorea

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