Journal of Plant Biology

, Volume 51, Issue 3, pp 167–173 | Cite as

Reactive oxygen species, antioxidants and signaling in plants

  • Parvaiz Ahmad
  • Maryam Sarwat
  • Satyawati Sharma


Several reactive oxygen species (ROS) are continuously produced in plants as byproducts of many metabolic reactions, such as photosynthesis, photo respiration and respiration, Depending on the nature of the ROS species, some are highly toxic and rapidly detoxified by various cellular enzymatic and nonenzymatic mechanisms. Oxidative stress occurs when there is a serious imbalance between the production of ROS and antioxidative defence. ROS participate in signal transduction, but also modify cellular components and cause damage. ROS is highly reactive molecules and can oxidize all types of cellular components. Various enzymes involved in ROS-scavenging have been manipulated and over expressed or down regulated. An overview of the literature is presented in terms of primary antioxidant free radical scavenging and redox signaling in plant cells. Special attention is given to ROS and ROS-anioxidant interaction as a metabolic interface for different types of signals derived from metabolisms and from the changing environment.


antioxidants gene expression MAPK signaling ROS 


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Literature cited

  1. Abreu IA, Saraiva LM, Soares CM, Teixeira M, Cabelli DE (2001) The mechanism of superoxide scavenging byArchaeoglobus fulgidus neelarredoxin. J Biol Chem276: 38995–39001PubMedCrossRefGoogle Scholar
  2. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev Plant Biol55: 373–99PubMedCrossRefGoogle Scholar
  3. Boo YC, Jung J (1999) Water deficit induced oxidative stress and Antioxidative defence in rice plants. J Plant Physiol51: 255–261Google Scholar
  4. Bray EA, Bailey-Serres J, Weretilnyk E (2000) Responses to abiotic stresses. In: BB Buchanan, W Gruissem, RL Jones, eds, Biochemistry and Molecular Biology of Plants. ASPR Rockvitle, pp 1158Google Scholar
  5. Causton HC, Ren B, Koh SS, Harbison CT, Kanin E, Jennings EC, Lee TI, True HL, Lander ES, Young RA (2001) Remodeling of yeast genome expression in response to environmental changes. Mol. Biol. Cell12: 323–37PubMedGoogle Scholar
  6. Chen D, Toone WM, Mata J, Lyne R, Burns G, Kivinen K, Brazma A, Jones N, Bahler J, (2003) Global transcriptional responses of fission yeast to environmental stress. Mol. Biol. Cell14: 214–29PubMedCrossRefGoogle Scholar
  7. Christman MF, Morgan RW, Jacobson FS, Ames BN (1985) Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins inSalmonella typhimurium. Cell,41: 753–762PubMedCrossRefGoogle Scholar
  8. Creissen G, Firmin J, Fryer M, Kular B, Leyland N, Reynolds H, Pastori G, Wellburn F, Baker N, Wellburn A, Mullineaux P (1999). Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress. Plant Cell11: 1277–92PubMedCrossRefGoogle Scholar
  9. del Rio LA, Sandalio LA, Corpus FJ, Lopez-Huertas E, Palma JM, Pastori GM (1998) Activated oxygen mediated metabolic functions of the peroxisomes. Physiol Plant104: 673–680CrossRefGoogle Scholar
  10. Desikan R, Mackerness S, Hancock JT, Neill SJ (2001) Regulation of theArabidopsis transcriptome by oxidative stress. Plant Physiol127: 159–72PubMedCrossRefGoogle Scholar
  11. Desikan R, Neill SJ, Hancock JT (2000) Hydrogen peroxide-induced gene expression inArabidopsis thaliana. Free Rad. Biol. Med28: 773–78PubMedCrossRefGoogle Scholar
  12. Desikan R, Reynolds A, Hancock JT, Neill SJ (1998) Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on gene expression inArabidopsis suspension cultures. Biochem. J.330: 115–120PubMedGoogle Scholar
  13. Fecht-Christoffers MM, Maier P, Horst WJ (2003) Apoplastic peroxidases and ascorbate are involved in manganese toxicity and tolerance ofVigna unguiculate. Physol Plant117: 237–244CrossRefGoogle Scholar
  14. Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of aging. Nature408: 239–247PubMedCrossRefGoogle Scholar
  15. Foyer CH, Noctor G (2005) Redox homeostis and antioxidant signaling: A metabolic interface between stress perception and physiological responses. Plant Cell17: 1866–1875PubMedCrossRefGoogle Scholar
  16. Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol141: 341–345PubMedCrossRefGoogle Scholar
  17. Gasch A, Spellman P, Kao C, Harel O, Eisen M, Storz G, Botsteim D, Brown P (2000) Genomic expression programs in the response of yeast cells to environmental changes. Mol. Biol. Cell11: 4241–57PubMedGoogle Scholar
  18. Harinasut P, Poonsopa D, Roengmongkoi K, Charoensataporn R (2003) Salt effects on antioxidant enzymes in mulberry cultivar. Science Asia29: 109–113CrossRefGoogle Scholar
  19. Hsu SY, Kao CH (2003) The protective effect of free radical scavengers and metal chelators on polyethylene glycol-treated leaves. Biol Plant46: 617–619CrossRefGoogle Scholar
  20. Jonak C, Ökresz L, Bögre L, Hirt H (2002) Complexity, cross talk and integration of plant MAP kinase signaling. Curr. Opin. Plant Biol5: 415–24PubMedCrossRefGoogle Scholar
  21. Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen J, Mullineaux PC (1999) Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis. Science284: 654–57PubMedCrossRefGoogle Scholar
  22. Keles Y, Oncel I (2002) Response of antioxidative defence system to temperature and water stress combinations in wheat seedlings. Plant Sci163: 783–790CrossRefGoogle Scholar
  23. Kiffin R, Bandyopadhyay U, Cuervo AM (2006) Oxidative stress and autophagy. Antioxidants and Redox Slg8: 152–162CrossRefGoogle Scholar
  24. Kovtun Y, Chiu WL, Tena G, Sheen J (2000) Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc. Natl. Acad. Sci. USA97: 2940–45PubMedCrossRefGoogle Scholar
  25. Kozaki A, Takeba G (1996) Photorespiration protects C3 plants from photooxidation. Nature384: 557–560CrossRefGoogle Scholar
  26. Lee DH, Lee CB (2000) Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. Plant Sci159: 75–85PubMedCrossRefGoogle Scholar
  27. Lee YP, Kim SH, Bang JW, Lee HS, Kwak SS, Kwon SY (2007) Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts. Plant Cell Rep26: 591–598PubMedCrossRefGoogle Scholar
  28. Liang YC (1999) Effects of silicon on enzyme activity and sodium, potassium and calcium concentration in barley under salt stress. Plant Soil209: 217–224CrossRefGoogle Scholar
  29. Lopez-Huertas E, Corpus FJ, Sandalio LM, del Rio LA (1999) Characterization of membrane polypeptides from pea leaf peroxisomes involved in superoxide radical generation, Biochem J337: 531–536PubMedCrossRefGoogle Scholar
  30. McCord JM (2000) The evolution of free radicals and oxidative stress. Am J Med108: 652–659PubMedCrossRefGoogle Scholar
  31. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci7: 405–410PubMedCrossRefGoogle Scholar
  32. Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci9: 1360–1385CrossRefGoogle Scholar
  33. Möller IM (2001) Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Ann Rev Plant Physiol Plant Mol Biol52: 561–591CrossRefGoogle Scholar
  34. Moon H, Lee B, Choi G, Shin D, Prasad T, Lee O, Kwak SS, Kim DH, Nam J, Bahk J, Hong JC, Lee SY, Cho MJ, Lim CO, Yun DJ (2003) NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc. Natl. Acad. Sci. USA100: 358–63PubMedCrossRefGoogle Scholar
  35. Moseyko N, Zhu T, Chang HS, Wang X, Feldman LJ (2002) Transcription profiling of the early gravitropic response inArabidopsis using high-density oligonucleotide probe microarrays. Plant Physiol130: 720–28PubMedCrossRefGoogle Scholar
  36. Mullineaux PM, Karpiniski S, Baker NR (2006) Spatial dependence for hydrogen peroxide-directed signaling in light-stressed plants. Plant Physiol14: 346–350CrossRefGoogle Scholar
  37. Nagamiya K, Motohashi T, Nakao K, Prodhan SH, Hattori E, Hirose, Ozawa K, Ohkawa Y, Takabe T, Takabe T, Komamine A (2007) Enhancement of salt tolerance in transgenic rice expressing anEscherichia coli catalase gene,kat E. Plant Biotechnol Rep1: 49–5CrossRefGoogle Scholar
  38. Nobuhiro S, Mittler R (2006) Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction. Physiol. Plant.126: 45–51CrossRefGoogle Scholar
  39. Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Ann Rev Plant Physiol Plant Mol Biol49: 249–279CrossRefGoogle Scholar
  40. Pfannschmidt T, Schutze K, Fey V, Sherameti I, Oelmuller R (2003) Chloroplast redox control of nuclear gene expression-A new class of plastid signals in interorganellar communication. Antioxidants and Redox Sig5: 95–101CrossRefGoogle Scholar
  41. Polle A (2001) Dissecting the superoxide dismutase-ascorbate-glu-tathione-pathway in chloroplasts by metabolic modeling. Computer simulations as a step towards flux analysis. Plant Physiol126: 445–462PubMedCrossRefGoogle Scholar
  42. Pourcel L, Routaboul JM, Cheynier V (2007) Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends in Plant Sci12: 29–36CrossRefGoogle Scholar
  43. Rizhsky L, Hallak-Herr E, Van Breusegem F, Rachmilevitch S, Barr JE, Rodermel S, Inze D, Mittler R (2002) Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate peroxidase or catalase. Plant J32: 329–42PubMedCrossRefGoogle Scholar
  44. Sanan-Mishra N, Tuteja R, Tuteja N (2006) Signaling through MAP kinase networks in plants. Arch Biochem Biophys452: 55–68CrossRefGoogle Scholar
  45. Sarowar S, Kim EN, Kim YJ, Ok SH, Kim KD, Hwang BK, Shin JS (2005) Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens. Plant Sci.169: 55–63CrossRefGoogle Scholar
  46. Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiol101: 7–12PubMedGoogle Scholar
  47. Schena M, Shalon D, Davis R, Brown P (1995) Quantitative monitoring of gene expression patterns with complementary DNA microarray. Science270: 467–470PubMedCrossRefGoogle Scholar
  48. Serres JB, Mittler R (2006) The Roles of Reactive Oxygen Species in Plant Cells. Plant Physiology 2006;141: 311CrossRefGoogle Scholar
  49. Shaaltiel Y, Chua NH, Gepstein S, Gressel J (1988) Dominant pleiotropy controls enzymes co-segregating with paraquet resistance inConyza bonariensis. Theor Appl Genet75: 850–856Google Scholar
  50. Shalata A, Mittova V, Volokita M, Guy M, Tal M (2001) Response of the cultivated tomato and its wild salt-tolerant relativeLycopersicon pennellii to salt-dependent oxidative stress: the antioxidative system. Physiol Plant.112: 487–494PubMedCrossRefGoogle Scholar
  51. Shao HB, Chu LY, Wu G, Zhang JH, Lu ZH, Hu YC (2007) Changes of some anti-oxidative physiological indices under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at tillering stage. Biointerfaces59: 113–119CrossRefGoogle Scholar
  52. Smirnoff N (2000) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol125: 27–58CrossRefGoogle Scholar
  53. Spychalla JP, Desbough SL (1990) Superoxide dismutase, Catalase, and alpha-tocopherol content of stored potato tubers. Plant Physiol94: 1214–1218PubMedCrossRefGoogle Scholar
  54. Srivalli B, Chinnusamy V, Khanna-Chopra R (2003) Antioxidant defense in response to abiotic stresses in plants, J Plant Biol30: 121–139Google Scholar
  55. Stohr C, Stremlau S (2006) Formation and possible roles of nitric oxide in plant roots. J Exp Bot57: 463–470PubMedCrossRefGoogle Scholar
  56. Sudhakar C, Lakshmi A, Giridarakumar S (2001) Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCI salinity. Plant Sci16: 613–619CrossRefGoogle Scholar
  57. Van Breusegem F, Vranova E, Dat JF, Inzé D (2001) The role of active oxygen species in plant signal transduction. Plant Sci161: 405–414CrossRefGoogle Scholar
  58. Vranova E, Atichartpongkul S, Villarroel, Van Montagu M, Inze D, Van Camp W (2002) Comprehensive analysis of gene expression inNicotiana tabacum leaves acclimated to oxidative stress. Proc. Natl. Acad. Sci. USA99: 10870–75PubMedCrossRefGoogle Scholar
  59. Wu G, Wei ZK, Shao HB (2007) The mutual responses of higher plants to environment: physiological and microbiological aspects. Biointerfaces59: 113–119CrossRefGoogle Scholar
  60. Zhang T, Liu V, Xue L, Xu S, Chen T, Yang T, Zhang L, An L (2006) Molecular cloning and characterization of a novel MAP kinase gene inChorispora bungeana. Plant Physiol Biochem44: 78–84PubMedCrossRefGoogle Scholar
  61. Zheng M, Wang X, Templeton L, Smulski D, LaRossa R, Storz G (2001) DNA microarray-mediated transcriptional profiling of theEscherichia coli response to hydrogen peroxide. Journal of Bacteriol183: 4562–4570CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Korea 2008

Authors and Affiliations

  • Parvaiz Ahmad
    • 1
  • Maryam Sarwat
    • 2
  • Satyawati Sharma
    • 1
  1. 1.Biochemistry laboratory, CRDTIndian Institute of TechnologyNew DelhiIndia
  2. 2.Plant Molecular BiologyICGEBNew DelhiIndia

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