Advertisement

Journal of Plant Biology

, Volume 48, Issue 4, pp 380–386 | Cite as

Growth characteristics and catalpol production in chinese foxglove (Rehmannia glutinosa Liboschitz) hairy roots transformed withAgrobacterium rhizogenes ATCC15834

  • Sung Jin Hwang
Article

Abstract

Hairy root clones ofRehmannia glutinosa were established via transformation withAgrobacterium rhizogenes ATCC15834. To optimize the culturing conditions for both root growth and catalpol production, effects of various combinations of seven basal media, pH, and carbon sources were examined under darkness. The fastest root growth was obtained in an SH medium containing 4% sucrose (pH 5.8); the highest catalpol content (0.54% of dry weight) was achieved in a WPM medium supplemented with 4% sucrose (pH 5.8). Effects of plant growth regulators and chitosan were also investigated. IAA at 2 mg L-1 significantly increased root lengths and the frequency of lateral roots. Chitosan (50 mg L-1) and CA3 (0.5 mg L-1) induced catalpol production, with contents calculated at 0.7% dry weight and 0.65% dry weight, respectively.

Keywords

catalpol chitosan hairy root cultures PGRs Rehmannia glutinosa 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. Amorim HV, Dougall DK, Sharp WR (1977) The effect of carbohydrate and nitrogen concentration on phenol synthesis in Paul’s Scarlet Rose cells grown in tissue culture. Physiol Plant39: 91–94Google Scholar
  2. Bakkali AT, Jaziri M, Ishimaru K, Tanaka N, Shimomura K, Yoshimatsu K, Homes J, Vanhaelen M (1997) Tannin production in hairy root cultures ofLawsonia inermis. J Plant Physiol151: 505–508Google Scholar
  3. Balandrin MJ, Klock JA (1998) Medicinal, aromatic and industrial materials from plants,In IY Bajaj, ed, Biotechnology in Agriculture and Forestry, 4. Medicinal and Aromatic Plants. Springer-Verlag, Berlin, pp 1–36Google Scholar
  4. Battat E, Rokem JS, Goldgerg I (1989) Growth ofDioscorea deltoides at high sugar concentrations. Plant Cell Rep7: 652–654Google Scholar
  5. Bhadra R, Vani S, Shank JV (1993) Production of indoie alkaloids by selected hairy root lines ofCatharanthus roseus. Biotechnol Bioeng41: 581–592PubMedCrossRefGoogle Scholar
  6. Enders R (1994) Plant Cell Biotechnology. Springer-Verlag, Berlin, pp 149–163Google Scholar
  7. Funk C, Brodelius P (1990) Influence of growth regulators and an elicitor on phenylpropanoid metabolism in suspension cultures ofVanilla plantifolia. Phytochem29: 845–848CrossRefGoogle Scholar
  8. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res50: 151–158PubMedCrossRefGoogle Scholar
  9. Giri A, Banerjee S, Ahuja PS, Giri CC (1997) Production of hairy roots inAconitum heterophyllum Wall, usingAgrobacterium rhizogenes. In Vitro Cell Dev Biol Plant33: 280–284CrossRefGoogle Scholar
  10. Hamill JD, Parr AJ, Robins RJ, Rhodes MJC (1986) Secondary product formation by cultures ofBeta vulgaris andNicotiana rustica transformed withAgrobacterium rhizogenes. Plant Cell Rep5: 111–114CrossRefGoogle Scholar
  11. Hwang SJ, Kim KS, Pyo BS, Hwang B (1999) Saponin production by hairy root cultures ofPanax ginseng CA Meyer. Biotechnol Bioprocess Eng4: 309–312CrossRefGoogle Scholar
  12. Ikeda T, Matsumoto T, Noguchi M (1976) Effects of nutritional factors on the formation of ubiquinone by tobacco plant cells in suspension cultures. Agric Biol Chem40: 1765–1770Google Scholar
  13. Ikenaga T, Oyama T, Muranaka T (1995) Growth and steroidal saponin production in hairy root cultures ofSolanum aculeatissimum. Plant Cell Rep14: 413–417CrossRefGoogle Scholar
  14. Ismailoglu UB, Saracoglu I, Harput US, Sahin-Erdemli I (2002) Effects of phenylpropanoid and iridoid glycosides on free radical-induced impairment of endothelium-dependent relaxation in rat aortic rings. J Ethnopharmacol79: 193–197PubMedCrossRefGoogle Scholar
  15. Jung KH, Kwak SS, Kim SW, Lee H, Choi CY, Liu JR (1992) Improvement of the catharanthine productivity in hairy root cultures ofCatharanthus roseus by using monosaccharides as a carbon source. Biotechnol Lett14: 695–700CrossRefGoogle Scholar
  16. Kittipongpatana N, Hock RS, Porter JR (1998) Production of solasodine by hairy root, callus, and cell suspension cultures ofSolanum aviculare Forst. Plant Cell Tiss Org Cult52: 133–143CrossRefGoogle Scholar
  17. Lindsey K, Yeomann MM (1983) The relation between growth rate, differentiation and alkaloid accumulation in cell cultures. J Exp Bot34: 1055–1065CrossRefGoogle Scholar
  18. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant18: 100–127CrossRefGoogle Scholar
  19. Liu C, Wang Y, Guo C, Ouyang F, Ye H, Li G (1998) Enhanced production of artemisinin byArtemisia annua L. hairy root cultures in a modified inner-loop airlift bioreactor. Bioprocess Eng19: 389–392Google Scholar
  20. Liu CZ, Wang YC, Outang F, Ye HC, Li GF (1997) Production of artemisinin by hairy root cultures ofArtemisia annua L. Biotechnol Lett19: 927–929CrossRefGoogle Scholar
  21. Lloyd G, McCown B (1980) Commercially feasible micropropagation of Mountain laurel,Kalmia latifolia, by use of shoot tip culture. Comb Proc Int Plant Prop Soc30: 421–427Google Scholar
  22. Mano Y, Ohkawa H, Yamada Y (1989) Production of tropane alkaloids by hairy root cultures ofDuboisia leichhardtii transformed byAgrobacterium rhizogenes. Plant Sci59: 191–201CrossRefGoogle Scholar
  23. Merkli A, Christen P, Kapetanidis I (1997) Production of diosgenin by hairy root cultures ofTrigonella foenumgraecum L. Plant Cell Rep16: 632–636CrossRefGoogle Scholar
  24. Mizukami H, Konoshima M, Tabata M (1977) Effects of nutritional factors on shikonin derivative formation inLithospermum callus cultures. Phytochem16: 1183–1187CrossRefGoogle Scholar
  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant15: 473–479CrossRefGoogle Scholar
  26. Nguyen C, Bourgaud F, Forlot P, Guckert A (1992) Establishment of hairy root cultures ofPsoralea species. Plant Cell Rep11: 424–497CrossRefGoogle Scholar
  27. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science164: 85–87CrossRefGoogle Scholar
  28. Park CH, Seong NS, Kim SK, Paek KY (1999) Characteristics in tissue cultured plants of Chinese foxglove(Rehmannia glutinosa Libosch.). Kor J Plant Tiss Cult26: 205–210Google Scholar
  29. Rhodes MJ, Parr AJ, Gjulietti A, Aird EL (1994) Influence of exogenous hormones on the growth and secondary metabolite formation in transformed root cultures. Plant Cell Tiss Org Cult38: 143–151CrossRefGoogle Scholar
  30. Rhodes MJ, Robins RJ, Hamill JD, Parr AJ, Hilton MJ, Walton NJ (1990) Properties of transformed root cultures,In BV Charlwood, MJ Rhodes, eds, Secondary Products from Plant Tissue Culture. Clarendon Press, Oxford, pp 201–225Google Scholar
  31. Robins RJ, Bent ES, Rhodes MJC (1991) Studies on the biosynthesis of tropane alkaloids byDatura stramonium L. transformed root cultures: 3. The relationship between morphological integrity and alkaloid biosynthesis. Planta185: 385–390CrossRefGoogle Scholar
  32. Sato K, Yamazaki T, Okuyama E, Yoshihira K, Shimomura K (1991) Anthroquinone production by transformed root culture ofRubia tinctorum. Phytochem30: 1507–1509CrossRefGoogle Scholar
  33. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot50: 199–204CrossRefGoogle Scholar
  34. Takeatsu K, Paul PHB, Guo JX, Chung KS, Han BH (1996) International Collection of Traditional and Folk Medicine, Vol 1. World Scientific, Singapore, pp 148–150Google Scholar
  35. Toivonen L (1993) Utilization of hairy root cultures for production of secondary metabolites. Biotechnol Prog9: 12–20CrossRefGoogle Scholar
  36. Wysokinska H, Chmiel A (1997) Transformed root cultures for biotechnology. Acta Biotechnol2: 131–159CrossRefGoogle Scholar
  37. Yamamoto O, Kamura K (1997) Production of saikosaponin in cultured roots ofBupleurum falcatum L. Plant Tiss Cult Biotech3: 139–147Google Scholar
  38. Zenk MH, El-Shagi H, Schutle U (1975) Anthroquinone production by cell suspension cultures ofMorinda citrifolia. Planta Med Suppl 79Google Scholar
  39. Zhu UP (1998) Tonifying herbs,In Chinese Materia Medica. Harwood Academic Pub, Groningen, pp 583–384Google Scholar

Copyright information

© The Botanical Society of Korea 2005

Authors and Affiliations

  1. 1.Department of Oriental Medicine MaterialsDongshin UniversityNajuKorea

Personalised recommendations