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Journal of Plant Biology

, Volume 48, Issue 1, pp 25–31 | Cite as

Comparison of cytotoxic activities betweenin vitro and field grown plants ofTyphonium flagelliforme (Lodd.) blume

  • Lai Keng ChanEmail author
  • Wan Yee Koh
  • Tengku S. Tengku-Muhammad
Article

Abstract

Anin vitro cytotoxicity screening of theTyphonium flagelliforme extracts indicated high cytotoxicity effect on human lung carcinoma NCl-H23 cells and human mammary gland carcinoma T-47D cells, but the extracts were not active on human liver carcinoma HepG2 cells. NCl-H23 cells were more susceptible toT. flagelliforme extracts than T-47D cells. EDP50 values of the hexane fractions of the mature plant and thein vitro plantlet ofT. flagelliforme on NCl-H23 cells were less than 2 μg/mL Extract from the mature plant was relatively more cytotoxic than the one fromin vitro plantlet except for the hexane fraction. The chloroform and butanol fraction of the mature plant had higher cytotoxicity effect than the fraction fromin vitro plantlet on NCl-H23 cells. All the 3 fractions (hexane, chloroform, and butanol) of the mature plant exhibited higher cytotoxicity effects on human mammary gland carcinoma T-47D cells than the 3 fractions ofin vitro plantlet. However, the human liver carcinoma cells were resistant toT. flagelliforme extracts except for higher concentration of hexane fractions of both the mature and thein vitro plants and the chloroform fraction of the mature plant. Micropropagated plantlets ofT. flagelliforme could hence be used as herbal materials for the treatment of human lung and breast cancers.

Keywords

carcinoma cells cytotoxicity ED50 values in vitro plantlets Typhonium flagelliforme 

Abbreviations

IBA

indole-3-butyric acid

MS

Murashige and Skoog

BA

N6-benzyladenine

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Copyright information

© The Botanical Society of Korea 2005

Authors and Affiliations

  • Lai Keng Chan
    • 1
    Email author
  • Wan Yee Koh
    • 1
  • Tengku S. Tengku-Muhammad
    • 1
  1. 1.School of Biological SciencesUniversiti Sains MalaysiaMinclenMalaysia

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