Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots ofEchinacea angustifolia

  • Chun-Hua Wu
  • Yaser Hassan Dewir
  • Eun-Joo Hahn
  • Kee-Yoeup Paek


We investigated different concentrations of auxins (1AA, IBA, NAA), the strength of the MS medium, sucrose and ammonium/nitrate contents, initial medium pH, and inoculum size to determine their effects on biomass increase and the accumulation of total phenols and flavonoids in adventitious roots ofEchinacea angustifolia. These roots were cultured under darkness in shake flasks for 4 weeks. IBA proved the best auxin for inducing root proliferation. Root growth was inhibited when the initial pH was maintained below 5.0 or above 6.0. Nitrate, rather than ammonium, was more necessary for root growth and phenolics accumulations. Overall, biomass increased and total phenol and flavonoid contents were maximized under the following conditions: half-strength MS medium supplemented with 2 mg L-1 IBA, 5% (w/v) sucrose, 5:25 (mM) ammonium/nitrate ratio, pH adjusted to 6.0 before autoclaving, and an inoculum size of 10 g L-1 FW. These results indicate that the type ofin vitro environment strongly affects growth and the accumulation of phenolics from adventitious root cultures ofE. angustifolia. Such optimization is beneficial to large-scale production of biomass and secondary metabolites in that species.


adventitious root culture Echinacea angustifolia flavonoids phenols shake flask culture 

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Copyright information

© The Botanical Society of Korea 2006

Authors and Affiliations

  • Chun-Hua Wu
    • 1
    • 2
  • Yaser Hassan Dewir
    • 1
    • 3
  • Eun-Joo Hahn
    • 1
  • Kee-Yoeup Paek
    • 1
  1. 1.Research Center for the Development of Advanced Horticultural TechnologyChungbuk National UniversityCheong-juKorea
  2. 2.Dalian Agricultural Science Research InstituteDalianChina
  3. 3.Department of Horticulture, Faculty of AgricultureTanta UniversityKafr El-SheikhEgypt

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