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Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots ofEchinacea angustifolia

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Abstract

We investigated different concentrations of auxins (1AA, IBA, NAA), the strength of the MS medium, sucrose and ammonium/nitrate contents, initial medium pH, and inoculum size to determine their effects on biomass increase and the accumulation of total phenols and flavonoids in adventitious roots ofEchinacea angustifolia. These roots were cultured under darkness in shake flasks for 4 weeks. IBA proved the best auxin for inducing root proliferation. Root growth was inhibited when the initial pH was maintained below 5.0 or above 6.0. Nitrate, rather than ammonium, was more necessary for root growth and phenolics accumulations. Overall, biomass increased and total phenol and flavonoid contents were maximized under the following conditions: half-strength MS medium supplemented with 2 mg L-1 IBA, 5% (w/v) sucrose, 5:25 (mM) ammonium/nitrate ratio, pH adjusted to 6.0 before autoclaving, and an inoculum size of 10 g L-1 FW. These results indicate that the type ofin vitro environment strongly affects growth and the accumulation of phenolics from adventitious root cultures ofE. angustifolia. Such optimization is beneficial to large-scale production of biomass and secondary metabolites in that species.

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Korea

    Chun-Hua Wu, Yaser Hassan Dewir, Eun-Joo Hahn & Kee-Yoeup Paek

  2. Dalian Agricultural Science Research Institute, 116036, Dalian, China

    Chun-Hua Wu

  3. Department of Horticulture, Faculty of Agriculture, Tanta University, 33516, Kafr El-Sheikh, Egypt

    Yaser Hassan Dewir

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  1. Chun-Hua Wu
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  2. Yaser Hassan Dewir
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  3. Eun-Joo Hahn
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  4. Kee-Yoeup Paek
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Correspondence to Kee-Yoeup Paek.

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Wu, CH., Dewir, Y.H., Hahn, EJ. et al. Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots ofEchinacea angustifolia . J. Plant Biol. 49, 193–199 (2006). https://doi.org/10.1007/BF03030532

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  • DOI: https://doi.org/10.1007/BF03030532

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