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Journal of Plant Biology

, Volume 47, Issue 3, pp 194–202 | Cite as

Influence of plant extracts and microbioagents on physiological traits of faba bean infected withBotrytis fabae

  • Yehia A. -G. Mahmoud
  • Mohsen K. H. EbrahimEmail author
  • Magda M. Aly
Article

Abstract

Laboratory and greenhouse experiments were conducted to assess the efficacy ofEucalyptus citriodora, Ipomoea carnea, Cuminum cyminum, Allium sativum and Hyoscyamus muticus leaf extracts, and Streptomyces exfloliatus (S) andTrichoderma harzianum (T) for controllingBotrytis fabae causing chocolate spot disease of faba bean. Laboratory study supported the use ofE. citriodora (Ex 1) andI. carnea (Ex 2) extracts than the others for controlling the growth ofB. fabae. S+T was the best for inhibiting spore germination followed by Ex 1 +Ex 2 after 8 h of testing, whereas Ex 1 +Ex 2 produced the lowest percent of germination after 16 h. After 4 days, the inhibiting order of the growth ofB. fabae was S+T > Ex 1 +Ex 2 > T > Ex 2 > Ex 1 =S. Greenhouse experiments showed the highest activities of peroxidase, catalase and pectinase in the infected plants. These activities were markedly reduced in healthy plants and widely changed by the biocontrol treatments. Applying biocontrol agents to the infected plants increased minerals (N, P, K and Mg), and both Chl biosynthesis and the photosynthetic activity, which in turn led to accumulation of metabolites. This served the plant to resist the detrimental effects ofB. fabae on the plant growth and yield. In this concern, the efficiency of test biocontrol agents seemed to be in the order:T+S > Ex 1 +Ex 2 >T >S > Ex 2 > Ex.

Keywords

photosynthesis plant extracts Streptomyces Trichoderma Vicia faba 

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Literature Cited

  1. Abu-Grab OS, Ebrahim MKH (2000) Physiological response of field-grown onion to some growth regulators. Egypt J Hort 27: 117–130Google Scholar
  2. Agwa H, El-Shanshoury AR, Aly M, Bonaly R (2000) Isolation and characterization of two Streptomyces species producing antifungal agents. J Union Arab Biol 9: 283–303Google Scholar
  3. Allen SG, Grimshaz HM, Parkinson JA, Quarmby C (1974) Chemical analysis of ecological materials. Blackwell Scientific Publishing, OxfordGoogle Scholar
  4. Aly MM, El-Sabbagh SM, El-Shouny WA, Ebrahim MKH (2004) Physiological response of Zea mays to NaCI stress with respect to Azotobacter chroococcum and Streptomyces niveus. Pakis J Biol Sci (in press)Google Scholar
  5. Arnon Dl (1949) Copper enzymes in isolated choloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15PubMedCrossRefGoogle Scholar
  6. Basham HG, Bateman DF (1975) Relationship of cell death, in plant tissue treated with a homogenous endopectate lyase, to cell wall degradation. Plant Pathol 5: 249–254CrossRefGoogle Scholar
  7. Biswal UC, Mohanty P (1976) Aging induced changes in photosynthetic electron-transport of detached barley leaves. Plant Cell Physiol 17: 323–326Google Scholar
  8. Cown MM (1999) Plant products as antimicrobial agents. Clinical Microbiol Rev 12: 564–582Google Scholar
  9. Duncan DB (1955) Multiple range test and multiple E test. Biometrics 11: 1–42CrossRefGoogle Scholar
  10. Ebrahim MKH, Vogg G, Osman MEH, Komor E (1998) Photosynthetic performance and adaptation of sugarcane at suboptimal temperatures. J Plant Physiol 153: 587–592Google Scholar
  11. Ghisalberti EL, Narbey MJ, Dewan MM, Sivasin K (1990) Varability among strains of Trichoderma harzianum in their ability to reduce take-all and to produce pyrones. Plant Soil 121: 287–291CrossRefGoogle Scholar
  12. Ismail IMK, Salama AM, Ali MIA, Ouf SA (1987) Effect of some phenolic compounds on spore germination and germ-tube length of Aspergillus fumigatus and Fusarium oxysporium. Cryptog Mycol 8: 51–57Google Scholar
  13. Jacobs MB (1958) The chemical analysis of food and food products. D.Van Nostrand, New YorkGoogle Scholar
  14. Jadhave PS, Malik NG, Chavan PD (1997) Allelopathic effects of Ipomoea carnea subsp. fistulosa on growth of wheat, rice, sorghum and kidney bean. Allelopathy J 4: 345–348Google Scholar
  15. Kato M, Shimizu S (1987) Chlorophyll metabolism in higher plants. VII. Cholorophyll degradation in senesc-ing tobacco leaves; phenolic-dependent peroxidative degradation. Can J Bot 65: 729–735CrossRefGoogle Scholar
  16. Katyal JC, Randhawa NS (1983) Zinc in plants.In JC Katyal and NS Randhawa, eds, Micronutrients Fert Plant Nutr Bull 7, FAO of the United Nations. Rome, pp 3–22Google Scholar
  17. Khaled AA, Abd El-Moity SMH, Omar SAM (1995) Chemical control of some faba bean diseases with fungicides. Egypt J Agric Res 73: 45–56Google Scholar
  18. Krause GH, Santarius KA (1975) Relative thermostability of the choloroplast envelope. Planta 127: 285–299CrossRefGoogle Scholar
  19. Kurucheve V, Ezhilan JG, Jayarai J (1997) Screening of higher plants fungitoxicityRhizoctonia solani in vitro. Ind J Phytopathol 50: 235–241Google Scholar
  20. Kvyatkovskii AF (1988) The effect of trace elements on nitrate reductase activity and cholorophyll content in maize leaves under irrigation. Fiziol Biochem Kult Past 20: 39–42Google Scholar
  21. Lorito MCK, Peterbauer CK, Hayes CK, Herman GE (1994) Synergistic interaction between fungal cell-wall degrading enzymes and different antifungal compounds on spore germination. Microbiol. 140: 623–629CrossRefGoogle Scholar
  22. Lowry OH, Rosebrough NJ, Farr LA, Randall RJ (1951) Protein measurements with the folin-phenol reagent. J Biol Chem 193: 265–275PubMedGoogle Scholar
  23. Mansfield JW, Deverall BJ (1974) The rate of fungal development and lesion formation in leaves of Vicia faba during infection by Botrytis fabae. Ann Appl Biol 79: 77–89CrossRefGoogle Scholar
  24. Misaghi IJ (1982) Physiology and biochemistry of plant pathology interaction. New York Pub Corp, New YorkGoogle Scholar
  25. Moran R, Porath D (1980) Cholorophyll determination in intact tissues using N, N-dimethyl formamide. Plant Physiol 65: 478–479PubMedCrossRefGoogle Scholar
  26. Naguib Ml (1963) Colorimetric estimation of plant polysaccharides. Zucker 16:15–18Google Scholar
  27. Naguib Ml (1964) Effect of sevin on carbohydrate and nitrogen metabolism during germination of cotton seeds. Ind J Expt Bid 2: 149–152Google Scholar
  28. Omar SAM, Chapman GR Bailtss KW (1985) Interaction between virus andBotrytis fabae inVicia fabae, In Proc 8-th Botrytis Symp. Quad Vitic Enol Univ, Torino, pp 243–248Google Scholar
  29. Osman MEA, El-Shintinawy F (1988) Photosynthetic electron-transport under phosphorylating conditions as influenced by different concentrations of various salts. J Exp Bot 39: 859–863CrossRefGoogle Scholar
  30. Page AL (1982) Chemical and microbiological properties,In AL Page, DE Baker, J Roscoe-Ellis, DR Keeney, Rl Miller, JD Rhoades, eds, Methods of Soil Analysis, Ed 2. Madison, pp 288–290Google Scholar
  31. Rothrock CS, Gottlieb D (1984) Role of antibiosis antagonism ofStreptomyces hygroscopicus toRhizoctonia solani in soil. Can J Microbiol 30: 1440–1447CrossRefGoogle Scholar
  32. Sattar EA, Gala A, Rashwan O (1995) Caffeoyl derivatives from the seeds ofIpomoea fistulosa. Int J Pharmacognosy 33: 155–158CrossRefGoogle Scholar
  33. Shirling EB, Gottlieb D (1966) Methods for characterizationof Streptomyces species. Int J Sys Bacterid 16: 313–340CrossRefGoogle Scholar
  34. Singh J, Faull JL (1990) Hyperparasitism and biological control,In KG Mukerji, KL Garg, eds, Biocontrol of Plant Pathogens. CRC Press, Boca Raton pp 167–179Google Scholar
  35. Singh RK, Dwivedi RS (1987) Effect of oils onSclerotiumn rolfsii causing root rot of barley. Ind J Phytopath 40: 531–533Google Scholar
  36. Somogyi M (1952) Notes on sugar determination. J Biol Chem 195: 19–23Google Scholar
  37. Tarrad AM, El-Hyatemy YY, Omar SA (1993) Wyerone derivatives and activities of peroxidase and phenoloxi-dase in faba bean leaves as induced by chocolate spot disease. Plant Sci 89: 161–165CrossRefGoogle Scholar
  38. Williams PF (1978) Growth of broad beans infected withBotrytis fabae. J Hort Sci 50: 415–424Google Scholar

Copyright information

© The Botanical Society of Korea 2004

Authors and Affiliations

  • Yehia A. -G. Mahmoud
    • 1
  • Mohsen K. H. Ebrahim
    • 1
    Email author
  • Magda M. Aly
    • 2
  1. 1.Botany Department, Faculty of ScienceTanta UniversityTantaEgypt
  2. 2.Biology Department, Faculty of Education, Kafr El-SheikhTanta UniversityTantaEgypt

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