Journal of Plant Biology

, 42:310 | Cite as

Binary vectors for efficient transformation of rice

  • Sichul Lee
  • Jong -Seong Jeon
  • Ki -Hong Jung
  • Gynheung An
Article

Abstract

We constructed binary vectors that were designed for transfer and expression of a gene into rice chromosomes. The binary vectors contained the hygromycin-resistance gene for selection of transformants and multiple-cloning sites within the transfer DNA. In addition, vectors were designed to express foreign genes using four kinds of promoters. We also report a procedure for efficient transformation of rice plants using scutellum-derived calli and theAgrobacterium strain LBA4404.

Keywords

Agrobacterium binary vector LBA4404 monocots rice transformation 

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Copyright information

© The Botanical Society of Korea 1999

Authors and Affiliations

  • Sichul Lee
    • 1
  • Jong -Seong Jeon
    • 1
  • Ki -Hong Jung
    • 1
  • Gynheung An
    • 1
  1. 1.Department of Life Science and National Research Laboratory of Plant Functional GenomicsPohang University of Science and Technology (POSTECH)PohangKorea

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