Journal of Plant Biology

, Volume 42, Issue 4, pp 266–272 | Cite as

Expression of theRSI-1 gene during development of roots and reproductive organs in tomato

  • Hawk -Bin Kwon
  • Gyung -Hee Lee
  • Jong -Joo Cheong
Article
Received:
Accepted:
  • 186 Downloads

Abstract

TheRSI-1 gene is expressed in pericycle cells just prior to the first round of cell division for lateral root development in tomato. We transformed tomato plants with theRSI-1 gene promoter linked to a GUS reporter gene. GUS activity was detected not only at the sites of initiation for lateral and adventitious roots, but also at the primary root tip. Expression of the fusion gene was also regulated at various stages of tissue development: in particular, during the formation of reproductive organs such as pollen and fruit Overexpression of theRSI-1 gene in either the sense or antisense orientation resulted in arrest of fruit development and seed germination. TheRSI-1 gene product, therefore, may play a role in auxin-induced cell division in various developing tissues. Inter and intramolecular disulfide bridges between cysteines rich in the RSI-1 protein might be involved in cell-wall modifications that are essential for new cell division. These hypotheses for the role of theRSI-1 gene in lateral-root and reproductive-organ development remain to be tested.

Keywords

auxin lateral root reproductive organs tomato transgenic plant 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. Aubert D, Chevillard M, Dome A-M, Arlaud G, Herzog M (1998) Expression patterns ofGASA genes inArabidopsis thaliana: theGASA4 gene is up-regulated by gibber-ellins in meristematic regions. Plant Mol Biol36: 871–883PubMedCrossRefGoogle Scholar
  2. Ben-Nissan G, Weiss D (1996) The petunia homologue of tomato gastl: transcript accumulation coincides with gibberellin-induced corolla cell elongation. Plant Mol Biol32: 1067–1074PubMedCrossRefGoogle Scholar
  3. Bevan MW (1984) BinaryAgrobacterium vectors for plant transformation. Nucleic Acids Res12: 8711–8721PubMedCrossRefGoogle Scholar
  4. Blakely LM, Blakely RM, Colowit PM, Elliott DS (1988) Experimental studies on lateral root formation in radish seedling roots, II. Analysis of the dose-response to exogenous auxin. Plant Physiol87: 414–419PubMedCrossRefGoogle Scholar
  5. Celenza JL, Grisafi PL, Fink GR (1995) A pathway for lateral root formation inArabidopsis thaliana. Genes Dev9: 2131–2142PubMedCrossRefGoogle Scholar
  6. Charlton WA (1991) Lateral root initiation,In Y Waisel, A Eshel, U Kafkafi, eds, Plant Roots, The Hidden Half, Marcel Dekker Inc, New York, pp 103–128Google Scholar
  7. Cheong J-J, Lee G-H, Kwon H-B (1999) Expression and regulation of theRSI-1 gene during lateral root initiation. J Plant Biol42: 259–265CrossRefGoogle Scholar
  8. Gordewener J, van der Zandt H, van Engelen F, van Kammen A, de Vries S (1991) Tunicamycin-inhibited carrot somatic embryogenesis can be restored by secreted cationic peroxidase isoenzymes. Pianta184: 478–486Google Scholar
  9. Dietrich RA, Radke SE, Harada JJ (1992) Downstream DNA sequences are required to activate a gene expressed in the root cortex of embryos and seedlings. Plant Cell4: 1371–1382PubMedCrossRefGoogle Scholar
  10. Fillatti JJ, Kiser J, Rose R, Comai L (1987) Efficient transfer of a glyphosate tolerance gene into tomato using a binaryAgrobacterium tumefaciens vector. Bio/Technology5: 726–730CrossRefGoogle Scholar
  11. Garbers C, Simmons C (1994) Approaches to understanding auxin action. Trends Cell Biol4: 245–250PubMedCrossRefGoogle Scholar
  12. Gee MA, Hagen G, Guilfoyle TJ (1991) Tissue-specific and organ-specific expression of soybean auxin-responsive transcriptsGH3 andSAURs. Plant Cell3: 419–430PubMedCrossRefGoogle Scholar
  13. Guilfoyle T, Hagen G, Ulmasov T, Murfett J (1998) How does auxin turn on genes? Plant Physiol118: 341–347PubMedCrossRefGoogle Scholar
  14. Herzog M, Dome A-M, Grellet F (1995)GASA, a gibberel-lin-regulated gene family fromArabidopsis thaliana related to the tomatoGAST1 gene. Plant Mol Biol27: 743–752PubMedCrossRefGoogle Scholar
  15. Hinchee MAW, Rost TL (1986) The control of lateral roots development in cultured pea seedlings, I: The role of seedling organs and plant growth regulators. Bot Gaz147: 137–147CrossRefGoogle Scholar
  16. Kares C, Prinsen E, Van Onckelen H, Often L (1990) IAA synthesis and root induction withiaa genes under heat shock promoter control. Plant Mol Biol15: 225–236PubMedCrossRefGoogle Scholar
  17. Keller B, Lamb CJ (1989) Specific expression of a novel cell wall hydroxyproline rich glycoprotein gene in lateral root initiation. Genes Dev3: 1639–1646PubMedCrossRefGoogle Scholar
  18. Klee JH, Horsch RB, Hinchee MA, Hein MB, Hoffmann NL (1987) The effects of overproduction of twoAgro-bacterium tumifaciens T-DNA auxin biosynthetic gene products in transgenic petunia plants. Genes Dev1: 86–96CrossRefGoogle Scholar
  19. Laskowski MJ, Williams ME, Nusbaum HC, Sussex IM (1995) Formation of lateral root meristems is a two-stage process. Development121: 3303–3310PubMedGoogle Scholar
  20. Lee G-H, Rodgers L, Taylor BH (1995) Glucuronidase as a marker for clonal analysis of tomato lateral roots. Transgenic Res4: 123–131CrossRefGoogle Scholar
  21. Macdonald H (1997) Auxin perception and signal transduction. Physiol Plant100: 423–430CrossRefGoogle Scholar
  22. Maclsaac SA, Sawhney VK, Pohorecky Y (1989) Regulation of lateral root formation in lettuce (Lactuca sativa) seedling roots: interacting effects of naphthaleneacetic acid and kinetin. Physiol Plant77: 287–293CrossRefGoogle Scholar
  23. Mersereau M, Pazour GF, Das A (1990) Efficient transformation ofAgrobacterium tumefaciens by electroporation. Gene90: 140–151CrossRefGoogle Scholar
  24. Mito N, Bennett AB (1995) Thediageotropica mutation and synthetic auxins differentially affect the expression of auxin-regulated genes in tomato. Plant Physiol109: 293–297PubMedCrossRefGoogle Scholar
  25. Shi L, Cast RT, Gopalraj M, Olszewski NE (1992) Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato. Plant J2: 153–159PubMedGoogle Scholar
  26. Showalter AM (1993) Structure and function of plant cell wall proteins. Plant Cell5: 9–23PubMedCrossRefGoogle Scholar
  27. Taylor BH, El-Kheir RA (1993) Induction of lateral root initiation by auxin in tomato seedling roots. Tomato Genet Coop Rep43: 46–48Google Scholar
  28. Taylor BH, Scheuring CF (1994) A molecular marker for lateral root initiation: TheRSI-1 gene of tomato (Lycopersicon esculentum Mill.) is activated in early lateral root primordia. Mol Gen Genet243: 148–157PubMedGoogle Scholar
  29. van Engelen FA, de Vries SC (1992) Extracellular proteins in plant embryogenesis. Trends Genet8: 66–70PubMedGoogle Scholar
  30. Walker L, Estelle M (1998) Molecular mechanisms of auxin action. Curr Opin Plant Biol1: 434–439PubMedCrossRefGoogle Scholar

Copyright information

© The Botanical Society of Korea 1999

Authors and Affiliations

  • Hawk -Bin Kwon
    • 1
  • Gyung -Hee Lee
    • 2
  • Jong -Joo Cheong
    • 3
  1. 1.Molecular Genetics DivisionNational Institute of Agricultural Science and TechnologySuwonKorea
  2. 2.Department of BiochemistryBrandeis UniversityWalthamUSA
  3. 3.Plant Cell Biotechnology LaboratoryKorea Research Institute of Bioscience and BiotecnologyTaejonKorea

Personalised recommendations