Improved production of caffeic acid derivatives in suspension cultures ofEchinacea purpurea by medium replenishment strategy
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The aim of this study was to produce caffeic acid derivatives from adventitious root cultures ofEchinacea purpurea, which are of high pharmaceutical value. The effects of both media optimization and replenishment strategies were adopted to achieve improved production ofE. purpurea adventitious roots and caffeic acid derivatives. Of the different media strengths (0.25 MS, 0.5 MS, 0.75 MS and 1 MS) tested for the culturing of adventitious roots in 5 L bioreactors, 0.5 MS medium was found to be most suitable for the growth of adventitious roots. The optima accumulation of biomass (73.6 g L-1 FW and 10.03 g L-1 DW), phenolics (61.14 mg g-1 DW) and flavonoids (38.30 mg g-1 DW) were achieved in this medium. Furthermore, fed batch cultivations (media replenishment with 0.25 MS, 0.5 MS, 0.75 MS and 1 MS at the end of 2nd and 3rd weeks) to further enhance the production of adventitious root biomass and metabolites were also attempted. High adventitious root biomasses (83.1 g L-1 FW and 15.30 g L-1 DW) were achieved with feeding of the 0.5 MS medium at the end of 2nd week. This led to slight decreases in the total production of phenolics and flavonoids; however, this feeding was responsible for increases in the accumulation of caftaric acid (5.76 mg g-1 DW) and cichoric acid (26.12 mg g-1 DW).
Key wordsAdventitious root cultures Caffeic acid derivatives Caftaric acid Chlorogenic acid Cichoric acid Echinacea
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- Bauer, R. and Wagner, H., Echinacea species as potential immunostimulatory drugs.Econ. Medic. Plant Res., 5, 253–321 (1991).Google Scholar
- Folin, O. and Ciocalteu, V, On tyrosine and tryptophane determination in proteins.J. Bio. Chem., 27, 627–650 (1927).Google Scholar
- King, P. J., Ma, M., Miao, W., Jia, Q., McDougall, B. R., Reinecke, M. G., Cornell, C., Kuan, J., Kim, T. R., and Robinoson, E. W., Structure-activity relationships: Analogues of the dicaffeoyl-quinic and dicaffeoytartaric acids as potent inhibitors of human immunodeficiency virus type-1 integrase and replication.J. Med. Chem., 43, 497–509 (1999).CrossRefGoogle Scholar
- Lin, Z., Neamati, N., Zhao, H., Kiryu, Y., Turpin, J. A., Aberham, C., Strebel, K., Kohn, K., Witvrouw, M., Pannecouque, C., Debyser, Z., Clercq, E. D., Rice, W. G., Pommier, Y., and Burke, T. R., Chicoric acid analogues as HIV-1 integrase inhibitors.J. Med. Chem., 42, 1401–1414 (1999).PubMedCrossRefGoogle Scholar
- Yamamoto, O. and Kamura K., Production of saikosaponin in cultured roots ofBupleumm falcatum L.Plant Tissue Cult. Biotechnol., 3, 138–147 (1997).Google Scholar
- Yu, K. W., Hahn, E. J., and Paek, K. Y., Production of ginseng adventitious roots using bioreactors.Korean J. Plant Tissue Cult., 27, 309–315 (2000).Google Scholar
- Yu, K. W., Production of useful secondary metabolites through bioreactor culture of Korean ginseng (Panax ginseng C. A. Meyer). Ph. D. thesis, Chungbuk National University, Cheongju, South Korea (2000).Google Scholar