Biological Trace Element Research

, Volume 3, Issue 2, pp 107–115 | Cite as

Influence of ashing techniques on the analysis of trace elements in animal tissue

I. Wet ashing
  • Michael S. Clegg
  • Carl L. Keen
  • Bo Lönnerdal
  • Lucille S. Hurley
Original Articles


A multitude of methods exists at present for the solubilization of biological tissues for atomic absorption analysis. We have examined several common methods of wet ashing using NBS bovine liver in order to determine which acids, acid combinations, or bases should be used as digesting agents for accurate and precise measurement of iron, copper, zinc, and manganese. Nitric acid proved to be the most effective wet ashing agent. With nitric acid, mean concentrations for iron, copper, and zinc differed from NBS certified values by less than 1.5% while those for manganese differed by 4%.

Index Entries

Analysis of trace elements, by wet ashing trace element analysis, by wet ashing wet ashing techniques, in trace element analysis elemental analysis, trace, wet ashing in iron, analysis by wet ashing copper, analysis by wet ashing manganese, analysis by wet ashing 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    G. D. Christian,Anal. Chem. 41, 24A (1969).PubMedCrossRefGoogle Scholar
  2. 2.
    L. Wurts, J. Smeyers-Verbcke, and D. L. Massart,Clin. Chim. Acta 72, 405 (1976).CrossRefGoogle Scholar
  3. 3.
    C. A. Johnson,Anal. Chim. Acta 81, 69 (1976).PubMedCrossRefGoogle Scholar
  4. 4.
    E. Bonilla,Clin. Chem. 24, 471 (1978).PubMedGoogle Scholar
  5. 5.
    J. Locke,Anal. Chem. Acta 104, 225 (1979).CrossRefGoogle Scholar
  6. 6.
    National Bureau of Standards, Washington, DC (1977).Google Scholar
  7. 7.
    N. I. Ward, R. Stephens, and D. E. Ryan.Anal. Chim. Acta 110, 9 (1979).CrossRefGoogle Scholar
  8. 8.
    T. T. Gorsuch,The Destruction of Organic Matter. 1st ed., Pergamon Press, Oxford, New York, 1970, p. 20.Google Scholar
  9. 9.
    C. L. Keen, B. Lönnerdal, M. V. Sloan, and L. S. Hurley,J. Nutr. 110, 897 (1980).PubMedGoogle Scholar
  10. 10.
    L. Murthy, E. E. Menden, P. M. Eller, and H. G. Petering,Anal. Biochem. 53, 365 (1973).PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1981

Authors and Affiliations

  • Michael S. Clegg
    • 1
  • Carl L. Keen
    • 1
  • Bo Lönnerdal
    • 1
  • Lucille S. Hurley
    • 1
  1. 1.Department of NutritionUniversity of CaliforniaDavis

Personalised recommendations