Environmental Science and Pollution Research

, Volume 1, Issue 4, pp 205–208 | Cite as

On-line speciation of arsenical compounds in fish and mussel extracts by HPLC-ICP-MS

  • Sergio Caroli
  • Francesco La Torre
  • Francesco Petrucci
  • Nicola Violante
Research Articles


The separation and determination of sub-μmol/l levels of six environmentally significant As compounds was accomplished by means of high performance liquid chromatography (HPLC) combined online with inductively coupled plasma mass spectrometry (ICP-MS). The species of interest (arsenite and arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine) were quantified in fish and mussel extracts after separation on a Dionex AS7 column equipped with an on-guard AG7 column using a bicarbonate buffer as the mobile phase in a gradient mode. The species thus eluted were directly forwarded to the ICP-MS detector. The detection power of the overall system allows each As form to be determined at concentrations as low a 0.0013–0.0027 μmol 1−1.


High Performance Liquid Chromatography Inductively Couple Plasma Mass Spectrometry Arsenite Arsenic Speciation Arsenobetaine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Ecomed Publishers 1994

Authors and Affiliations

  • Sergio Caroli
    • 1
  • Francesco La Torre
    • 1
  • Francesco Petrucci
    • 1
  • Nicola Violante
    • 1
  1. 1.Istituto Superiore di SanitàRomeItaly

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