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Toxicity, uptake and metabolism of 4-n-nonylphenol in root cultures and intact plants under septic and aseptic conditions

  • Maria Bokern
  • Petra Raid
  • Hans Harms
Research Articles

Abstract

4-Nonylphenol, a compound with estrogenic activity, has been shown to occur in sewage sludges and effluents of sludge treatment. This, as well as its use in the formulation of pesticides, may result in the contamination of crop plants and may therefore have an impact on the quality of food or feedstuff. The toxicity, uptake and metabolism of 4-n-nonylphenol (4-n-NP) were investigated as14C-labeled 4-n-NP in root cultures under septical and aseptical conditions and with intact plants grown in containers with soil and aseptically grown in nutrient media. 4-n-NP was toxic to all plant systems tested. The presence of microorganisms and the developmental state of the plant material appeared to have an influence on the EC50 values. 4-n-NP was taken up by the roots and a metabolism to polar compounds was observed in the cases where sufficiently high uptake rates. With intact plants a transport from roots to the shoots was evident. Metabolism in roots changed quantitatively in the presence of microorganisms. The mineralization of 4-n-NP to14CO2 only occurred with microorganisms.

Keywords

Effluents of sludge treatment, estrogenic activity estrogenic activity, degradation estrogenic activity, microorganisms estrogenic activity, mineralization estrogenic activity, phytotoxicity sewage sludges, estrogenic activity 

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References

  1. [1]
    Berlin, J.;Fecker, L.;Rugenhagen, C.;Sator, C.;Strack, D.;Witte, L.;Wray, V. (1991): Isoflavone glycoside formation in transformed and non-transformed suspension and hairy root cultures ofLupinus polyphyllns andLupinus hartwegii. Z. Naturforsch. 46c, 725–734Google Scholar
  2. [2]
    Bokern, M.;Harms, H. (1997): Toxicity and metabolism of 4-n-nonylphenol in cell suspension cultures of different plant species. Environ. Sci. Technol. 31, 1849–1854CrossRefGoogle Scholar
  3. [3]
    Bokern, M.;Nimtz, M.;Harms, H. (1996): Metabolites of 4-n-nonylphenol in wheat cell suspension cultures. J. Agric. Food Chem. 44, 1123–1127CrossRefGoogle Scholar
  4. [4]
    Giger, W.;Brunner, P.H.;Schaffner C. (1984): 4-Nonylphenol in sewage sludge: Accumulation of toxic metabolites from nonionic surfactants. Science 225, 623–625CrossRefGoogle Scholar
  5. [5]
    Giger, W.;Stephanou, E.;Schaffner, C. (1981): Persistent organic chemicals in sewage effluents: 1. Identifications of nonylphenols and nonylphenolethoxylates by glass capillary gas chromatography/mass spectrometry. Chemosphere 19, 1253–1263CrossRefGoogle Scholar
  6. [6]
    Jobling, S.;Sheahan, D.;Osborne, J.A.;Matthiessen, P.;Sumpter, J.P. (1996): Inhibition of testicular growth on rainbow trout (Oncorhynchus mykiss) exposed to estrogenic alkylphenol chemicals. Environ. Toxicol. Chem. 15, 194–202CrossRefGoogle Scholar
  7. [7]
    Jobst, H. (1995): Chlorophenols and nonylphenols in sewage sludges. 1. Occurrence in sewage sludges of Western German treatment plants from 1987–1989. Acta Hydrochim. Hydrobiol.23, 20–25CrossRefGoogle Scholar
  8. [8]
    Harms, H.;Langebartels, C. (1986): Standardized plant cell suspension test systems for an ecotoxicologic evaluation of the metabolic fate of xenobiotics. Plant Science 45, 157–165CrossRefGoogle Scholar
  9. [9]
    Kirchmann, H.;Aström, H.;Jönsäll, G. (1991): Organic pollutants in sewage sludge. 1. Effect of toluene, naphtalene, 2-methylnaphtalene, 4-n-nonylphenol and di-2-ethylhexyl-phtalate on soil biological processes and their decomposition in soil. Swedish J. agric. Res. 21, 107–113Google Scholar
  10. [10]
    Langebartels, C. Harms, H. (1986): Cell suspension cultures as test systems for an ecotoxicologic evaluation of chemicals. Growth inhibition effects and comparison with the metabolic fate in intact plants. Angew. Bot. 60, 113–123Google Scholar
  11. [11]
    Novak, J.M.; Jayachandran, K.; Moorman, T.B.; Weber, J.B. (1995): Sorption and binding of organic compounds in soils and their relation to bioavailability. In: Bioremediation: Science and Applications. Soil Science Society of America, American Society of Agronomy, Crop Science Society (Eds.) p.13-31, Segoe Rd., Madison, WI 53711, USAGoogle Scholar
  12. [12]
    Purdom, C.E.;Hardiman, P.A.;Bye, V.J.;Eno, N.C.;Tyler, C.R.;Sumpter, J.P. (1994): Estrogenic effects of effluents from sewage treatment works. Chem. Ecol. 8, 275–285CrossRefGoogle Scholar
  13. [13]
    Soto, A.M.;Jusna A.H.;Wray, J.W.;Sonnenschein, C. (1991): p-Nonylphenol: an estrogenic xenobiotic released from “modified” polystyrene. Environ. Health Perspect. 92, 167–173CrossRefGoogle Scholar
  14. [14]
    Sweetman, A. J. (1994): Development and application of a multi-residue analytical method for the determination of n-alkanes, linear alkylbenzenes, polynuclear aromatic hydrocarbons and 4-nonylphenol in digested sewage sludges. Wat. Res. 28, 343–353CrossRefGoogle Scholar
  15. [15]
    Wang, M-J.;Bokern, M.;Böhme, C.;Jones, K.C.;Harms, H. (1996): Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells. Environm. Toxicol. Chem. 15, 109–114CrossRefGoogle Scholar
  16. [16]
    White, R.;Jobling, S.;Hoare, S.A.;Sumpter, J.P.;Parker, M.G. (1994): Environmentally persistent alkylphenolic compounds are estrogenic. Endocrinology 135, 175–182CrossRefGoogle Scholar
  17. [17]
    Wilken, A.;Bock, C.;Bokern, M.;Harms, H. (1995): Metabolism of different PCB congeners by plant cell cultures. Environ. Toxicol. Chem. 14, 2017–2022CrossRefGoogle Scholar

Copyright information

© Ecomed Publishers 1998

Authors and Affiliations

  • Maria Bokern
    • 1
  • Petra Raid
    • 1
  • Hans Harms
    • 1
  1. 1.Bundesforschungsanstalt für LandwirtschaftInstitut für Pflanzenernährung und BodenkundeBraunschweigGermany

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