Journal of Biosciences

, Volume 25, Issue 1, pp 19–23

Analysis of BRCA1 involvement in breast cancer in Indian women

Articles

Abstract

The involvement of the familial breast-ovarian cancer gene (BRCA1) in the molecular pathogenesis of breast cancer among Indian women is unknown. We have used a set of microsatellite polymorphisms to examine the frequency of allele loss at the BRCA1 region on chromosome 17q21, in a panel of 80 human breast tumours. Tumour and blood leukocyte/normal tissue DNA from a series of 80 patients with primary breast cancer was screened by PCR-amplified microsatellite length polymorphisms to detect deletions at three polymorphic BRCA1 loci. PCR-allelotype was valuable in examining allele losses from archival and small tumour samples. Loss of alleles at BRCA1 in the patient set, confirmed a noteworthy role of this gene in the molecular pathogenesis of breast cancer and was in accordance with its well-documented tumour suppressive function.

Keywords

BRCA1 breast cancer microsatellite polymorphisms tumour suppressive function 

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Copyright information

© Indian Academy of Sciences 2000

Authors and Affiliations

  1. 1.Division of Laboratory MedicineTata Memorial HospitalMumbaiIndia
  2. 2.Department of SurgeryTata Memorial HospitalMumbaiIndia

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