International Journal of Hematology

, Volume 77, Issue 1, pp 75–81 | Cite as

Effect of Granulocyte Colony-Stimulating Factor on Bone Metabolism During Peripheral Blood Stem Cell Mobilization

  • Tsutomu Watanabe
  • Hiroko Suzuya
  • Toshihiro Onishi
  • Sachiyo Kanai
  • Michiya Kaneko
  • Hiroyoshi Watanabe
  • Ryuji Nakagawa
  • Yoshifumi Kawano
  • Yoichi Takaue
  • Yasuhiro Kuroda
  • James E. Talmadge
Article

Abstract

Granulocyte colony—stimulating factor (G-CSF) has been shown to affect the biochemical markers of bone metabolism, including serum bone alkaline phosphatase (BALP), serum osteocalcin, and urine deoxypyridinoline. To determine the association between bone resorption and formation and the G-CSF—induced mobilization of peripheral blood stem cells (PBSC), we examined these markers during mobilization in 19 healthy donors. The average (± SEM) serum BALP level before treatment was 81.6 ± 17.0 IU/dL, and the level increased significantly to 117.7 ± 15.8 IU/dL on day 5 of G-CSF administration (P < .0001). The urine deoxypyridinoline level before treatment was 12.3 ± 2.4 nmol/mmol creatinine, and this level also increased significantly to 19.4 ± 3.0 nmol/mmol creatinine on day 5 of G-CSF administration (P < .0001). In contrast, the average level of serum osteocalcin significantly decreased from 8.07 ± 2.88 ng/mL to 1.53 ± 0.18 ng/mL on day 5 (P = .0353). During G-CSF administration, we also studied the serum levels of various cytokines (IL-lβ, osteoclastogenesis inhibitory factor [OCIF], IL-6, tumor necrosis factor a, transforming growth factor β, interferon-γ, macrophage colony—stimulating factor) related to bone metabolism. Only the kinetics of OCIF were significantly affected. The serum level of OCIF increased immediately after the start of G-CSF administration and remained high during G-CSF administration. These results demonstrate that high-dose G-CSF affects bone metabolism and that OCIF may play a role in bone metabolism. Consistent with the notion that G-CSF affects bone metabolism, a significant correlation was observed between CD34+ cell yield and the increase in urine deoxypyridinoline but not for the changes in serum BALP and osteocalcin levels. This result suggests that bone resorption is either directly or indirectly related to the mobilization of PBSC by G-CSF.

Key words

Peripheral blood stem cells Mobilization G-CSF Bone metabolism 

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Copyright information

© The Japanese Society of Hematology 2003

Authors and Affiliations

  • Tsutomu Watanabe
    • 1
  • Hiroko Suzuya
    • 1
  • Toshihiro Onishi
    • 1
  • Sachiyo Kanai
    • 1
  • Michiya Kaneko
    • 1
  • Hiroyoshi Watanabe
    • 1
  • Ryuji Nakagawa
    • 1
  • Yoshifumi Kawano
    • 2
  • Yoichi Takaue
    • 3
  • Yasuhiro Kuroda
    • 1
  • James E. Talmadge
    • 4
  1. 1.Department of PediatricsUniversity ofTokushima School of MedicineTokushimaJapan
  2. 2.Department of PediatricsKyushu Cancer CenterFukuoka
  3. 3.Department of Internal MedicineNational Cancer CenterTokyoJapan
  4. 4.Department of Pathology/MicrobiologyUniversity of Nebraska Medical CenterOmahaUSA

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