International Journal of Hematology

, Volume 73, Issue 1, pp 84–86

Elevated serum-soluble fas ligand in Histiocytic necrotizing lymphadenitis

  • Kei Kato
  • Koichi Ohshima
  • Keizo Anzai
  • Junji Suzumiya
  • Masahiro Kikuchia
Review Article


Recent studies have indicated that Fas and perforin-based mechanisms seem to induce apoptosis in histiocytic necrotizing lymphadenitis (HNL). In this study, we evaluated the serum levels of soluble Fas ligand (sFasL) in 30 HNL patients using paired sera. Elevations in sFasL levels were detected in 9 patients in the active stage. In the convalescent stage, the levels of sFasL decreased to an undetectable degree after 2 weeks.These findings indicate the possibility that sFasL plays an important role in the constitutional symptoms and pathogenesis of HNL and, furthermore, that it can act as a surrogate marker of the disease activity.

Key words

Histiocytic necrotizing lymphadenitis Fas ligand Soluble Fas ligand Perforin Apoptosis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kikuchi M. Lymphadenitis showing focal reticulum cell hyperplasia with nuclear debris and phagocytes [in Japanese].Acta Haematol Jpn. 1972;35:379–380.Google Scholar
  2. 2.
    Liu Q, Ohshima K, Shinohara T, Kikuchi M. Apoptosis in histiocytic necrotizing lymphadenitis.Pathol Int. 1995;45:729–734.CrossRefPubMedGoogle Scholar
  3. 3.
    Ohshima K, Kikuchi M, Sumiyoshi Y, et al. Proliferating cells in histiocytic necrotizing lymphadenitis.Virchows Arch B Cell Pathol. 1991;61:97–100.Google Scholar
  4. 4.
    Kägi D, Vignaux F, Ledermann B, et al. Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity.Science. 1994;265:528–530.CrossRefPubMedGoogle Scholar
  5. 5.
    Mori N, Yatabe Y, Asai J, Nagasawa T. Immunohistochemical study of necrotizing lymphadenitis: a possible mechanism for apoptosis involving perforin and granzyme-producing cytotoxic T cells.Pathol Int. 1997;47:31–37.CrossRefPubMedGoogle Scholar
  6. 6.
    Ohshima K, Shimazaki K, Kume T, Suzumiya J, Kanda M, Kikuchi M. Perforin and Fas pathways of cytotoxic T cells in histiocytic necrotizing lymphadenitis.Histopathol. 1998;33:471–478.CrossRefGoogle Scholar
  7. 7.
    Felgar RE, Furth EE, Wasik MA, Gluckman SJ, Salhany KE. Histiocytic necrotizing lymphadenitis (Kikuchi’s disease): in situ end-labeling, immunohistochemical, and serologic evidence supporting cytotoxic lymphocyte-mediated apoptotic cell death.Mod Pathol. 1997;10:231–41.PubMedGoogle Scholar
  8. 8.
    Suda T, Okazaki T, Naito Y, et al. Expression of the Fas ligand in cells of T cell lineage.J Immunol. 1995;154:3806–3813.PubMedGoogle Scholar
  9. 9.
    Suda T, Takahashi T, Golstein P, Nagata S. Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family.Cell. 1993;75:1169–1178.CrossRefPubMedGoogle Scholar
  10. 10.
    Tanaka M, Suda T, Takahashi T, Nagata S. Expression of the functional soluble form of human Fas ligand in activated lymphocytes.EMBO J. 1995;14:1129–1135.PubMedGoogle Scholar
  11. 11.
    Tanaka M, Suda T, Haze K, et al. Fas ligand in human serum.Nat Med. 1996;2:317–322.CrossRefPubMedGoogle Scholar
  12. 12.
    Kato K, Ohshima K, Ishihara S, Anzai K, Suzumiya J, Kikuchi M. Elevated serum soluble Fas ligand in natural killer cell proliferative disorders.Br J Haematol. 1998;103:1164–1166.CrossRefPubMedGoogle Scholar
  13. 13.
    Hasegawa D, Kojima S, Tatsumi E, et al. Elevation of the serum Fas ligand in patients with hemophagocytic syndrome and Diamond-Blackfan anemia.Blood. 1998;91:2793–2799.PubMedGoogle Scholar
  14. 14.
    Sieg S, Huang Y, Kaplan D. Viral regulation of CD95 expression and apoptosis in T lymphocytes.J Immunol. 1997;159:1192–1199.PubMedGoogle Scholar
  15. 15.
    Ogasawara J, Watanabe-Fukunaga R, Adachi M, et al. Lethal effect of the anti-Fas antibody in mice.Nature. 1993;364:806–809.CrossRefPubMedGoogle Scholar

Copyright information

© The Japanese Society of Hematology 2001

Authors and Affiliations

  • Kei Kato
    • 1
  • Koichi Ohshima
    • 1
  • Keizo Anzai
    • 2
  • Junji Suzumiya
    • 1
  • Masahiro Kikuchia
    • 1
  1. 1.First Department of PathologySchool of Medicine, Fukuoka UniversityJapan
  2. 2.Department of Laboratory MedicineSchool of Medicine, Fukuoka UniversityJapan
  3. 3.Department of Pathology, School of MedicineFukuoka UniversityJonan-KuJapan

Personalised recommendations