, 28:121 | Cite as

Isozyme and amplified fragment length polymorphisms fromCephalosporium maydis in Egypt

  • Kurt A. Zeller
  • James E. Jurgenson
  • Elhamy M. El-Assiuty
  • John F. Leslie
Research Papers


Isoenzyme and amplified fragment length polymorphisms (AFLP) variation within a set of 48 isolates ofCephalosporium maydis was characterized. These isolates included ten cultures that have served as standards in the Egyptian maize resistance breeding program and 38 additional strains collected from 11 governates in Egypt during the 1997 growing season. Eight isozymes also were tested, but only five (acid phosphatase, fumerase, gtucose 4-phosphate isomerase, isocitrate dehydrogenase, and malate dehydrogenase) produced identifiable bands and all five of these enzymes were monomorphic. Sixty-eight AFLP primer-pair combinations were used and 865 bands were scored, of which 288 (33%) were polymorphic and could be used to discriminate four distinct subgroups, or lineages. Representatives from only two of the four lineages are included in the set of ten strains that has been used to challenge new lines in the Egyptian maize breeding program. From among these 68 primer-pair combinations, we identified a set of four AFLP primer-pairs that were strongly correlated (Pearson‘sr > 0.85) with the full data set that can be used as markers to determine the distribution of these lineages and to identify new lineages in field populations.

Key Words

Clonal populations corn genetic diversity kw]isozymes late wilt maize stalk rot 


  1. 1.
    Abel-el-Rahim, M.F., Sabet, K.A., El-Shafey, H.A. and El-Assiuty, E.M. (1982) Chemical control of the late-wilt disease of maize caused byCephalosporium maydis. Agric. Res. Rev., Egypt 60:31 -49.Google Scholar
  2. 2.
    Anderson, J.B. and Kohn, L.M. (1995) Clonality in soilbome plant-pathogenic fungi.Annu. Rev. Phytopathol. 33:369–391.CrossRefPubMedGoogle Scholar
  3. 3.
    Casela, C.R., Ferreira, A.S., Zeller, K.A. and Levy, M (1995) Pathotype variation in the sorghum anthracnose fungus: a phylogenetic perspective for resistance Leslie, J.F. and Frederiksen, R.A. [Eds.] Disease Analysis through Genetics and Biotechnology: Interdisciplinary Bridges to Improved Sorghum and Millet Crops.pp. 257–2766. Iowa State University Press, Ames, Iowa, USA.Google Scholar
  4. 4.
    Chen, W.D. (1994) Vegetative compatibility groups ofVerticillium dahliae from ornamental woody plants.Phytopathology 84:214–219.CrossRefGoogle Scholar
  5. 5.
    Chen, X., Line, R.F. and Leung, H. (1993) Relationship between virulence variation and DNA polymorphism inPuccinia striiformis. Phytopathology 83:1489–1497.CrossRefGoogle Scholar
  6. 6.
    Correll, J.C, Klittich, C.J.R. and Leslie, J.F. (1987) Nitrate non-utilizing mutants ofFusarium oxysporum and their use in vegetative compatibility tests.Phytopathology 77:1640–1646.CrossRefGoogle Scholar
  7. 7.
    Dobinson, K.F., Patterson, N.A., White, G.J. and Grant, S. (1998) DNA fingerprinting and vegetative compatibility analysis indicate multiple origins forVerticillium dahliae race 2 tomato isolates from Ontario, Canada.Mycol. Res. 102:1089–1095.CrossRefGoogle Scholar
  8. 8.
    Drenth, A., Tas, I.C.Q. and Govers, F. (1994) DNA fingerprinting uncovers a new sexually reproducing population ofPhytophthora infestons in the Netherlands.Eur. J. Plant Pathol. 100:97–107.CrossRefGoogle Scholar
  9. 9.
    El-Assiuty, E.M., El-Shafey, H.A., Ismael, A.-S., Fahmy, Z.M. and El-Abedein, A.Z. (1998) Pathogenic variation inCephalosporium maydis. Phytopathology 88:S25.Google Scholar
  10. 10.
    El-Assiuty, E.M., Ismael, A.M., Zeller, K.A. and Leslie, J.F. (1999) Relative colonization ability of greenhouse-grown maize by four lineages ofCephalosporium maydis from Egypt.Phytopathology 89:S23.Google Scholar
  11. 11.
    Elias, K.S., Zamir, D., Lictman-Pleban, T. and Katan, T. (1993) Population structure ofFusarium oxysporum f.sp.lycopersicr: restriction fragment length polymorphisms provide genetic evidence that vegetative compatibility group is an indicator of evolutionary origin.Molec. Plant-Microbe Interact. 6:565–572.Google Scholar
  12. 12.
    El-ltriby, H.A., Khamis, M.N., El-Demerdash, R.M. and El-Shafey, H.A. (1984) Inheritance of resistance to late wilt(Cephalosporium maydis) in maize.Proc. 2nd Mediterranean Conf. on Genetics (Cairo, Egypt), pp. 29–44.Google Scholar
  13. 13.
    El-Shafey, H.A., Abd-el-Rahim, M.F., Abd-el-Azim, O.Z. and Abd-el-Hamid, M.S. (1976) Carry-over of maize stalk-rot fungi in seed.Agric. Res. Rev., Egypt 54:29–42.Google Scholar
  14. 14.
    El-Shafey, H.A., El-Shorbagy, F.A., Khalil, I.I. and El-Assiuty, E.M. (1988) Additional sources of resistance to the late-wilt disease of maize caused byCephalosporium maydis.Agric. Res. Rev., Egypt 66:221–230.Google Scholar
  15. 15.
    El-Shafey, H.A., Mahmoud, S.A.Z., El-Brolousy, M., El-Azhary, T.M. and Ibrahim, T.F. (1990) Biological control of maize late-wilt disease by inoculation withBacillus subtilis and manuring.Egypt. J. Appl. Sci. 5:182–191.Google Scholar
  16. 16.
    Gonzalez, M., Rodriguez, M.E.Z., Jacabo, J.L., Hernandez, F., Acosta, J., Martinez, O. and Simpson, J. (1998) Characterization of Mexican isolates ofColletotrichum lindemuthianum by using differential cultivars and molecular markers.Phytopathology 88:292–299.PubMedCrossRefGoogle Scholar
  17. 17.
    Hill, M., Witsenboer, H., Zabeau, M., Vos, P., Kesseli, R. and Michelmore, R. (1996) PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships inLactuca spp.Theor. Appl. Genet. 93:1202–1210.CrossRefGoogle Scholar
  18. 18.
    Huss, M.J., Campbell, C.L., Jennings, D.B. and Leslie, J.F. (1996) Isozyme variation in theGibberella fujikuroi species complex(Fusarium sectionLiseola).Appl. Environ. Microbiol. 62:3750–3756.PubMedGoogle Scholar
  19. 19.
    Jacobson, K.M., Miller, O.K., Jr. and Turner, B.J. (1993) Randomly amplified polymorphic DNA markers are superior to somatic incompatibility tests for discriminating genotypes in natural populations of the ectomycorrhizal fungusSuillus granulatus.Proc. Natl. Acad. Sci. USA 90:9159–9163.PubMedCrossRefGoogle Scholar
  20. 20.
    Janssen, P., Coopman, R., Huys, G., Swings, J., Bleeker, M., Vos, P., Zabeau, M. and Keraters, K. (1996) Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy.Microbiology 142:1881–1893.PubMedGoogle Scholar
  21. 21.
    Keréyi, Z., Zeller, K., Hornok, L. and Leslie, J.F. (1999) Standardization of mating-type terminology in theGibberella fujikuroi species complex.Appl. Environ. Microbiol. 65:4071–4076.Google Scholar
  22. 22.
    Kistler, H.C., Momol, E.A. and Benny, U. (1991) Repetitive genomic sequences for determining relatedness among strains ofFusarium oxysporum.Phytopathology 81:331–336.CrossRefGoogle Scholar
  23. 23.
    Leslie, J.F. (1993) Fungal vegetative compatibility.Annu. Rev. Phytopathol. 31:127–150.PubMedCrossRefGoogle Scholar
  24. 24.
    Levy, M., Correa-Victoria, F.J., Zeigler, R.S., Xu, S. and Hamer, J.E. (1993) Genetic diversity of the rice blast fungus in a disease nursery in Colombia.Phytopathology 83:1427–1433.CrossRefGoogle Scholar
  25. 25.
    Majer, D., Mithen, R., Lewis, B., Vos, P. and Oliver, R.P. (1996) The use of AFLP fingerprinting for the detection of genetic variation in fungi.Mycol. Res. 100:1107–1111.CrossRefGoogle Scholar
  26. 26.
    Meijer, G., Megnegneau, B. and Linders, E.G.A. (1994) Variability for isozyme, vegetative compatibility and RAPD markers in natural populations ofPhomopsis subordinaria.Mycol. Res. 98:267–276.Google Scholar
  27. 27.
    Milgroom, M.G. (1996) Recombination and the multilocus structure of fungal populations.Annu. Rev. Phytopathol. 34:457–477.PubMedCrossRefGoogle Scholar
  28. 28.
    Milgroom, M.G., Lipari, E.S. and Powell, W.A. (1992) DNA fingerprinting and analysis of population structure in the chestnut blight fungusCryphonectria parasitica.Genetics 131:297–306.PubMedGoogle Scholar
  29. 29.
    Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA.Nucleic Acids Res. 8:4321–4325.PubMedCrossRefGoogle Scholar
  30. 30.
    Sabet, K.A., Samra, A.S. and Khalil, I.K. (1966) Variation inCephalosporium maydis, the cause of the late wilt disease,in: Samra, A.S. and Sabet, K.A. [Eds.] Investigations on Stalk-Rot Diseases of Maize in U.A.R. pp. 46–93. Ministry of Agriculture, Government Printing Offices, Cairo, Egypt.Google Scholar
  31. 31.
    Sabet, K.A., Samra, A.S. and Mansour, I.M. (1966) Late-wilt of maize and a study of the causal Samra, A.S. and Sabet, K.A. [Eds.] Investigations on Stalk-Rot Diseases of Maize in U.A.R. pp. 8-45. Ministry of Agriculture, Government Printing Offices, Cairo, Egypt.Google Scholar
  32. 32.
    Sabet, K.A., Zaher, A.M., Samra, A.S. and Mansour, I.M. (1970) Pathogenic behavior ofCephalosporium maydis andC. acremonium.Ann. Appl. Biol. 66:257–263.CrossRefGoogle Scholar
  33. 33.
    Samra, A.S. and Sabet, K.A. (1966) An introductory note,in: Samra, A.S. and Sabet, K.A. [Eds.] Investigations on Stalk-Rot Diseases of Maize in U.A.R. pp. 1–7. Ministry of Agriculture, Government Printing Offices, Cairo, Egypt.Google Scholar
  34. 34.
    Samra, A.S., Sabet, K.A. and Abd-el-Rahim, M.F. (1966) Seed transmission of stalk-rot fungi and effect of seed treatment,in: Samra, A.S. and Sabet, K.A. [Eds.] Investigations on Stalk-Rot Diseases of Maize in U.A.R. pp. 94–116. Ministry of Agriculture, Government Printing Offices, Cairo, Egypt.Google Scholar
  35. 35.
    Samra, A.S., Sabet, K.A. and Hingorani, M.K. (1962) A new wilt disease of maize in Egypt.Plant Dis. Rep. 46:481–483.Google Scholar
  36. 36.
    Samra, A.S., Sabet, K.A. and Hingorani, M.K. (1963) Late wilt disease of maize caused byCephalosporium maydis.Phytopathology 53:402–406.Google Scholar
  37. 37.
    Samra, A.S., Sabet, K. A., Kamel, M. and Abd-el-Rahmin, M.F. (1971) Further Studies on the Effect of Field Conditions and Cultural Practices on Infection with Stalk-rot Complex of Maize. Arab Republic of Egypt, Min. of Agriculture, Plant Protection Dept., Bull. no. 2.Google Scholar
  38. 38.
    Shehata, A.H. and Salem, A.M. (1972) Genetic analysis of resistance to late-wilt of maize caused byCepluilosporium maydis.Sabrao. J. 4:1–5.Google Scholar
  39. 39.
    Sicard, D., Michalikis, Y., Dron, M. and Neema, C. (1997) Genetic diversity and pathogenic variation ofColletotrichum lindemuthianum in three centers of diversity of its host,Phaseolus vulgaris.Phytopathology 87:807–813.PubMedCrossRefGoogle Scholar
  40. 40.
    Spooner, D.M., Tivang, J., Nienhuis, J., Miller, J.T., Douches, D.S. and Contreras, M.A. (1996) Comparison of four molecular markers in measuring relationships among the wild potato relativesSolarium sectionEtuberosum (subgenusPotatoe).Theor. Appl. Genet. 92:532–540.CrossRefGoogle Scholar
  41. 41.
    Strausbaugh, CA., Schroth, M.N., Weinhold, A.R. and Hancock, J.G. (1992) Assessment of vegetative compatibility and virulence ofVerticillium dahliae isolates from Idaho potatoes and tester strains.Phytopathology 82:61–68.CrossRefGoogle Scholar
  42. 42.
    Swofford, D.L. (1998)PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0*. Sinauer Associates, Sunderland, MA, USA.Google Scholar
  43. 43.
    Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Homes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. (1995) AFLP: A new technique for DNA fingerprinting.Nucleic Acids Res. 23:4407–4414.PubMedCrossRefGoogle Scholar
  44. 44.
    Zeigler, R.S., Tohme, J., Nelson, R.J., Levy, M. and Correa-Victoria, F. (1994) Lineage exclusion: A proposal for linking blast population analysis to resistance breeding.Proc. Int. Symp. on Rice Blast Disease (International Rice Research Institute, Los Bailos, the Philippines), pp. 267–292.Google Scholar
  45. 45.
    Zeigler, R.S., Cuoc, L.X., Scott, R.P., Bernardo, M.A., Chen, D.H., Valent, B. and Nelson, R.J. (1995) The relationship between lineage and virulence inPyricularia grisea in the Philippines.Phytopathology 85:443–451.CrossRefGoogle Scholar
  46. 46.
    Zeller, K.A., Jurgenson, J.E. and Leslie, J.F. (1998) Genetic variation inCephalospoiium inaydis from Egypt.Phytopathology 88:S103.Google Scholar
  47. 47.
    Zeller, K.A., Jurgenson, J.E. and Leslie, J.F. (1999) AFLP markers reveal genetic variation in Egyptian populations ofCephalospoiium maydis.Fungal Genet. Newsl. 46(suppl):94.Google Scholar

Copyright information

© Springer Science + Business Media B.V. 2000

Authors and Affiliations

  • Kurt A. Zeller
    • 1
  • James E. Jurgenson
    • 2
  • Elhamy M. El-Assiuty
    • 3
  • John F. Leslie
    • 1
  1. 1.Dept. of Plant Pathology Throckmorton Plant Sciences Center, Kansas State UniversityUSA
  2. 2.Dept. of BiologyUniversity of Northern IowaUSA
  3. 3.Maize and Sorghum Diseases Section, Plant Pathology Research InstituteAgricultural Research CenterGizaEgypt

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