Phytoparasitica

, Volume 34, Issue 5, pp 463–473 | Cite as

Exploration for anti-insect properties of lectin from seeds of soybean (Glycine max) usingBactrocera cucurbitae as a model

  • Kuljinder Singh
  • Manpreet Kaur
  • Pushpinder J. Rup
  • Jatinder Singh
Entomology
Received:
Accepted:

Abstract

Lectin fromGlycine max L. was extracted and purified by affinity chromatography using asialofetuin-linked porous amino-activated silica beads. The concentration-dependent effect of lectin was studied on freshly laid eggs (0–8 h old) of the melon flyBactrocera cucurbitae (Coquillett); lectin failed to influence egg hatching. However, treating second instar larvae (64–72 h old) with increasing concentrations of lectin significantly reduced the development period, number of pupae and number of emergingB. cucurbitae, and was negatively correlated with the increase in the lectin concentration. The LC50 value, 54µg ml−1, was calculated on the basis of adult emergence. Treatment of the larvae (64–72 h old) with the LC50 concentration resulted in a decrease in pupal weight. The activity of three hydrolase enzymes (esterases, acid and alkaline phosphatases), one oxidoreductase (catalase) and one group transfer enzyme (glutathione S-transferase) was assayed in second instar larvae at the LC50 concentration of lectin after exposure for 24, 48 and 72 h. The activity of esterases increased significantly (P<0.01) at the three exposure intervals, whereas the activities of the three other hydrolyses and the transferases were significantly suppressed (P<0.01).

Key words

Bactrocera cucurbitae melon fly catalase esterases Glycine max soybean lectin phosphatases transferases 
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Copyright information

© Springer Science + Business Media B.V. 2006

Authors and Affiliations

  • Kuljinder Singh
    • 1
  • Manpreet Kaur
    • 2
  • Pushpinder J. Rup
    • 1
  • Jatinder Singh
    • 2
  1. 1.Dept. of Zoology, Insect Physiology LaboratoryGuru Nanak Dev UniversityAmritsarIndia
  2. 2.Dept. of Molecular Biology and Biochemistry, Protein LaboratoryGuru Nanak Dev UniversityAmritsarIndia

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